Sentences with phrase «with cells in a dish»

Biologists rely on animal models to answer important questions that can't be addressed with cells in a dish.

Not exact matches

To develop their «disease in a dish» model, the team took skin cells from patients with Allan - Herndon - Dudley syndrome and reprogrammed them into induced pluripotent stem cells, which then can be developed into any type of tissue in the body.

Furthermore, when healthy neurons in a dish were treated with serum from the patients and antibodies against leiomodin - 1, they did not survive, but removing the antibodies increased brain cell survival.

So far, researchers have mostly turned on genes with CRISPRa in cells growing in lab dishes, says Charles Gersbach, a biomedical engineer at Duke University not involved in the new study.

These techniques include: human tissue created by reprogramming cells from people with the relevant disease (dubbed «patient in a dish»); «body on a chip» devices, where human tissue samples on a silicon chip are linked by a circulating blood substitute; many computer modelling approaches, such as virtual organs, virtual patients and virtual clinical trials; and microdosing studies, where tiny doses of drugs given to volunteers allow scientists to study their metabolism in humans, safely and with unsurpassed accuracy.

That would be getting close to the number of cells in a mouse brain,» raising the distant prospect of a human brain organoid with cognitive and even emotional capacities, all while sitting in a lab dish.

In addition to looking at mouse models of diabetes, the researchers also showed that exposure of human pancreatic islet cells — both from healthy donors and from patients with Type 1 diabetes — to fasting - mimicking diet in a dish stimulated insulin productioIn addition to looking at mouse models of diabetes, the researchers also showed that exposure of human pancreatic islet cells — both from healthy donors and from patients with Type 1 diabetes — to fasting - mimicking diet in a dish stimulated insulin productioin a dish stimulated insulin production.

Zheng, together with Leah Boyer, then a researcher in Gage's lab and now director of Salk's Stem Cell Core, generated diseased neurons by taking skin cells from patients with Leigh syndrome, reprogramming them into stem cells in culture and then coaxing them to develop into brain cells in a dish.

Mouse brain nerve cells (green) making a disease - causing version of the tau protein were grown in lab dishes with supporting brain cells called glia.

The disease model, described in a new study by a UC San Francisco - led team, involves taking skin cells from patients with the bone disease, reprogramming them in a lab dish to their embryonic state, and deriving stem cells from them.

Xu and colleagues grew S. gallolyticus in lab dishes with several different types of human cells.

When combined with the compound, called ganciclovir, these loaded nanoparticles were 100 percent effective at killing glioma cells grown in laboratory dishes.

According to his unpublished findings, when he puts glioblastoma cells from patients into lab dishes with brain organoids, the cells attach to the surface of the organoids, burrow into them, and within 24 to 48 hours grow into a mass that eventually «looks exactly like what happened in the patient's own brain,» Fine said.

The research team tested the cells in dishes that were treated with luteolin and found that migration was inhibited by the plant compound.

They then put the dishes into special chambers called bioreactors that keep them warm and in gentle motion reminiscent of a womb, encouraging the cells to form blobs with working neurons and many other features of a full - size human brain.

In cells grown on flat culture dishes, the expression of thousands of genes didn't match up with their normal patterns, explaining why the cells from those dishes had been unable to generate new hair follicles.

Rather than stick the isolated cells on a flat culture dish, they mixed the cells with liquid, then let the mixture hang in tiny droplets from a plastic lid, like condensation on the roof of a container.

By performing experiments in petri dishes and with mice, they found that panobinostat, a drug designed to change the way cells regulate genes, may be effective at inhibiting DIPG growth and extending survival rates.

«We culture typical skin cell of the epidermis, such as human keratinocytes, in our dishes to form an artificial epidermis with all of its natural layers,» explained Sibylle Thude, the biologist who led the investigation into the accreditation.

«A unique property of the SCN is that if its cells are grown in a dish, they quickly synchronize their clocks with each another,» Blackshaw says.

The investigators studied application of various doses of a common PBDE flame retardant on human adrenal cells in culture dishes and compared the effects with those of only the vehicle, the inactive substance used to deliver the chemical.

Both normalized progeria cells in a dish and treated mice with a version of the disease.

In this case, the researchers used samples from juveniles and adults with Fragile X syndrome and induced the cells to become neurons in a lab disIn this case, the researchers used samples from juveniles and adults with Fragile X syndrome and induced the cells to become neurons in a lab disin a lab dish.

Two men with prostate cancer, cells in a petri dish, a loss of function that may or may not translate into pathogenicity: This does not merit my mental energy.

In lab dishes, Liu's team corrected a mutation in human cells from a patient with an iron - storage blood disorder called hereditary hemochromatosiIn lab dishes, Liu's team corrected a mutation in human cells from a patient with an iron - storage blood disorder called hereditary hemochromatosiin human cells from a patient with an iron - storage blood disorder called hereditary hemochromatosis.

The newfound protein might eventually help patients who suffer from the untreatable prion diseases: When the group mixed their artificial protein fragment with a fragment of the PrP protein that usually kills neurons in petri dishes, the mixture killed only half as many cells as the prion fragment alone.

They then combined these in a dish with testicular cells from newborn mice in various culture conditions — a process that took hundreds of trials, says author Xiao - Yang Zhao, a stem cell biologist now at Southern Medical University in Guangzhou, China.

They have spread lies about his methods, accused him of electrocuting monkeys when in fact he was working with cells in culture dishes, and limited his job opportunities.

The research builds on the team's previous work with a technique called three - dimensional culture, which involves incubating stem cells in a floating ball - shaped aggregate, unlike traditional cell culture in which cells grow in a flat layer on the surface of a culture dish.

Normally, CD4 cells will start dividing when they are treated with certain proteins in a culture dish.

When they loaded sperm cells with doxorubicin, a common chemo agent, and released them in a dish containing mini cervical cancer tumours, the sperm swam towards the tumours, killing 87 per cent of their cells within three days.

When Chang and Poh grew these synthetic E. coli in a dish with P. aeruginosa, the suicide bomber was able to kill 99 % of the P. aeruginosa cells, the researchers report today in Molecular Systems Biology.

The biggest problem for transplanted stem cells, Bulte says, is that they're initially grown in a dish with ready access to oxygen, then put in the body, where levels are relatively low.

COS - 7 cells, plated at 70 % confluence in 100 - mm tissue culture dishes, were transfected with vector alone or pcDNA3 - ACVR1 (c. 617G) or pcDNA3 - ACVR1 (c. 617A).

Next steps include He's collaboration with Piedmont Atlanta Hospital to retrieve T cells, liver cancer cells and healthy tissue normally removed from patients during surgery, put the mouse receptor genes on these T cells and monitor in a dish both how those cells now fight the tumor and react to healthy human tissue.

In initial work with cell cultures in Petri dishes, the researchers methodically ruled out the possibility that HIV / gp120 would be inducing the death of stem cells and determined instead that HIV / gp120 was acting by inhibiting stem cell proliferatioIn initial work with cell cultures in Petri dishes, the researchers methodically ruled out the possibility that HIV / gp120 would be inducing the death of stem cells and determined instead that HIV / gp120 was acting by inhibiting stem cell proliferatioin Petri dishes, the researchers methodically ruled out the possibility that HIV / gp120 would be inducing the death of stem cells and determined instead that HIV / gp120 was acting by inhibiting stem cell proliferation.

But making chimeras with human organs whose development can be studied is more likely to succeed than the technique researchers have been trying for years: coaxing stem cells growing in lab dishes to become three - dimensional, functional tissues and organs.

As the Joslin team reports in the journal Nature Medicine, the work began with taking samples of both brown and white precursor cells from four human subjects and genetically modifying these cells to «immortalize» them for long life in a Petri dish.

With our new technology, we can quickly create billions of these cells in a dish and then transplant them into damaged hearts to treat heart failure.»

To confirm these findings, the team infected human cells in dish with two forms of CMV — one of which could generate the IE2 accelerator circuit and one that could not.

Wang's team discovered that if human lung cancer cells in a lab dish in the presence of the receptor were treated with BCX, they migrated less than untreated ones.

When a dish of mammalian cells is transiently transfected with a plasmid containing a fluorescent protein (fusion) there is huge variation in the fluorescence intensities of individual mammalian cells within the dish.

Your culture dishes could be infected with Mycoplasma, tiny bacteria that thrive in cell culture environments.

Understanding this process enables Canals» lab to grow a huge number of neurons in dishes in the lab - useful both for basic research and also as a source of healthy cells for experimenting with cell transplantation.

The usual approach is to grow human cells in a dish, to infect and try to treat them there, but this is not possible with liver cells or hepatocytes.

For example, researchers have demonstrated that the method can be used to treat muscular dystrophy and glaucoma in mice, render human cells grown in lab dishes resistant to HIV, and help create food crops and livestock with desirable traits.

To determine the effect of the conditioned medium on NGF - induced neurite outgrowth, PC12 cells were dissociated and plated on polyornithine - coated tissue culture dishes in the following conditions: 1) HT22 conditioned medium, 2) J147 treated HT22 conditioned medium, 3) DMEM alone plus J147, 4) DMEM plus NGF at 50 nanograms per ml, 5) J147 treated HT22 conditioned medium pre-incubated for one hour with 10 μg / ml anti-NGF and N2 supplement (Invitrogen).

We can't predict which virus species will slip into eggs and sperm and provide us with the next piece of the human genome, but here is one fact that's pretty unsettling to ponder: If you put a koala retrovirus in a dish with human cells, it can easily infect them.

For definitive endoderm differentiation, spontaneously formed EBs on day 6 were plated onto matrigel - coated dishes and maintained in a low concentration of fetal bovine serum (FBS) in combination with a high concentration of recombinant Activin A. Scale - like cells appeared (Figure 5A), which were positive for definitive endoderm markers such as Foxa2, Sox17 and Gata4.

So you'd want many more cells in a dish that are beating and with the right cell type.