Sentences with phrase «with confocal»

These samples were analyzed with a confocal microscope system (Bio-Rad MRC 1024 coupled to a Zeiss Axiovert 135).

We thank D. Dorward and O. Steele - Mortimer for help with confocal microscopy; S. Raffel and G. Sylva for sequencing; T. Hackstadt for assistance with photography; M. Schrumpf for supplying protein lysates and E. coli strains; R. Gilmore, M. L. Mbow, and O. Steele - Mortimer for providing antibodies; K. Caughey, G. Hettrick, and A. Mora for graphic support; and F. DeLeo, T. Hackstadt, and S. Priola for critical review of the manuscript.

Purkinje cells labeled with GFP: XYZ image with confocal and super resolution image in different Z positions.

We would like to thank M. P. Hefferan for his help with confocal microscopy and M.L. Gage for editorial comments.

We would like to thank Dr. Peter Didier for the pathology report, Dr. Xavier Alvarez for assistance with confocal microscopy, and Dr. Nicholas Crossland for critical review of the manuscript.

The image was taken with a confocal laser scanning microscope and shows cells giving strong inmmunofluorescence staining for CD3 antigen (green), indicating presence of cells of T - lymphocytes origin in the infarct zone of the heart tissue, counterstained nuclei with DAPI (blue).

Images were taken with the confocal microscopes Zeiss LSM710 and image analysis was accomplished with Fiji (ImageJ).

The ultimate dream is to take STEM into three dimensions with confocal electron microscopy, which images a material in slices by changing the focus of the beam.

Arabidopsis root seen with confocal microscopy.

Not exact matches

Top scientists don't flap if they need to use a confocal microscope and have never tackled the beast before, they just find someone to teach them the basics and get on with it.

These include more sensitive mass spectrometry for detecting, identifying and quantifying protein molecules, electron microscopy for visualizing an assortment of components of the nano - machine that transports toxins, and fluorescence confocal microscopy, which can label proteins with light emitting dyes.

Confocal imaging gives us the opportunity to view the vascular system by illuminating the whole body with fluorescent light and providing a translucent image of the subject.

The Shot: Pan took this digital image with an Olympus FluoView FV1000 confocal microscope using a 20x objective and a photomultiplier tube.

The confocal microscopy of Setaria grass roots (top and bottom insets) show mutant strains of Azospirillum brasilense and Herbaspirillum serpedicae with heavy colonization around branch roots and the elongation of primary roots.

Confocal microscopy of human neural stem cell culture infected with Zika virus (red).

Using chemicals like these in combination with new imaging tools, such as the multi-photon confocal microscope, has enabled researchers to explore the minuscule world of the neuron and observe brain cells in action with far more precision.

Kozlowski tackled that problem computationally; using the popular MATLAB programming environment, she and her colleagues developed an algorithm that classifies the shapes of microglia in confocal microscopy images with 90 % accuracy.

Scientists get to play with great bits of kit like confocal microscopes and even get to take a peek right into the heart of nature.

«Our new approach, SDC - PAINT, integrates the versatile super-resolution capabilities of DNA - PAINT with the optical sectioning features of confocal microscopes.

Specifically, the MPI / Wyss Institute team developed the technique for «Spinning Disk Confocal» (SDC) microscopes that detect fluorescence signals from an entire plane all at once by sensing them through a rotating disc with multiple pinholes.

The figure shows a confocal microscopy image of a mammalian cell undergoing apoptosis with the mitochondria stained in magenta and the protein Bax in green.

In confocal microscopy the focal plane is imaged with a single, intense point of light, centered between two cones of light that flood the rest of the sample.

So the Bessel beam can scan a cell with the same total flux as the light spots used in confocal microscopy, but that flux is distributed among the seven beams and the cell isn't flooded with excess light; thus, the cell isn't damaged nearly as quickly.

The chip is compatible with optical imaging technologies such as confocal microscopy, allowing the researchers to obtain high - resolution images and movies of the fertilization process, as it occurs.

The automated confocal micro-extensometer (ACME)-- developed by a team of researchers in Europe and the US — allows scientists to measure spatial variation in the mechanical properties of plant cells with unprecedented accuracy.

Using confocal laser microscopy and electron microscopy, coupled with the use of advanced synchrotron techniques at the Swiss Light Source, in Villigen (Switzerland), and at the Canadian Light Source in Saskatoon, Canada, the team was able to show that not only the morphology of microbial twisted structures is preserved after long incubations under diagenetic conditions, but also organic remnants can be detected in the mineralized twisted structures.

Photobleaching and phototoxicity are typically reduced by one to two orders of magnitude relative to that seen with a 1D scanned Bessel beam or the point array scanned excitation of spinning disk confocal microscopy.

Z - stack images of taste buds and geniculate ganglia were collected on an Olympus Fluoview FV300 laser scanning confocal microscope with a 60 × oil - immersion objective [numerical aperture (NA) 1.3] and 20 × oil - immersion objective (NA 0.7) or on a Leica TCS SP5 laser scanning confocal microscope with a 63 × oil - immersion objective (NA 1.4).

«Nonlinear stage - scanning confocal microscopy is critical because it allows us to rapidly measure the nonlinear emission from thousands of different nanostructures while minimizing the potential systematic errors, such as intensity or beam pointing variations, often associated with tuning the wavelength of an ultrafast laser,» O'Brien says.

Korenberg's team is developing a 3D coordinate system to align various types of neuroimaging data in the macaque brain, from whole - brain MRI connectivity to single - cell confocal data and, for some areas, subcellular resolution with electron microscopy.

The CARS images were acquired with a Leica TCS SP8 CARS system (Leica Microsystems, Mannheim, Germany) consisting of a TCS SP8 confocal microscope combined with a picoEmerald laser (APE, Berlin, Germany) offering a fixed Stokes laser line of 1064.5 nm and a tuneable Pump line from an optical parametric oscillator (780 nm — 940 nm).

IDMIT is an infrastructure for preclinical research in infectious diseases and immunology which is certified ISO9001 and which includes 1) A large animal facility with capacity to host NHP in BSL2 and BSL3 containment, 2) State - of - the - art laboratories for cell biology, immunology, molecular biology, flow cytometry and mass cytometry (CyTof), cell - sorting and confocal microscopy in BSL3 containment; 3) A biological resources centre with high storage capacity; 4) Highly innovative technologies for in vivo imaging of large animals in BSL2 and BSL3 containment, including a two - photon microscope, a PET - CT facility, and several optic based technologies (fibered endo - microscopy, near infra - red imaging).

By labeling one of the shell proteins with Yellow Fluorescent Protein (YFP), we were able to use confocal fluorescence microscopy to observe punctuate loci forming inside chloroplasts.

Biologists, biochemists and geneticists who need to visualize the anatomical location of cellular structure, proteins / enzymes, mRNA, and reporter genes within whole tissue are among those who can benefit from this innovative product, which can be used with light microscopy, fluorescence microscopy, confocal microscopy and single / multiphoton microscopy.

It is essential to familiarize yourself with the microscope you are going to be working with, especially its emission / excitation / dichroic filters and, for confocal microscopes, its laser lines.

Combined with spinning disk confocal, TIRF, FLIM and photomanipulation, Marianas LightSheet is a powerful live - cell imaging workstation.

Images were acquired with a LSM710 Zeiss confocal laser scanning system or Olympus IX81 light microscope.

The 3D reconstruction over time of the forming blood clot was captured with multiple fluorescent probes using VIVO, both wide field deconvolution and Spinning Disk Confocal.

Confocal microscopy of AD macrophages responsive to curcuminoids showed surface binding in untreated macrophages but co-localization with phalloidin in an intracellular compartment after treatment.

Images were taken on a Zeiss LSM 510 confocal microscope (Carl Zeiss, Canada) equipped with Argon and Helium - Neon lasers.

In this webinar, the principles of state - of - the - art confocal Raman imaging as a tool for the analysis of molecular characteristics of a sample will be presented, then the manner in which this information can be linked to structural information acquired with scanning electron microscopy will be demonstrated.

Combined with spinning confocal, it enables Super Resolution to be achieved at high speed and low photo - toxicity, making it the ideal solution for live high resoluion cell imaging.

It is one of the market leaders in compound and stereo microscopy, digital microscopy, confocal laser scanning microscopy with related imaging systems, electron microscopy sample preparation, and surgical microscopes.

The Clarity LFC Laser Free Confocal unit from Aurox uses a patented optical system, based on a unique design of spinning disc with a grid - like structured illumination pattern.

CrestOptics is a Manufacturing Company with the mission to develop advanced methodologies and equipment aimed at the MicroBiology Research, Material Sciences and Diagnostics The first Company milestone (2009), has been the development and manufacturing of the Advanced Confocal Attachment «X-Light», based on state of the art solutions in spinning disk technology, optics and engineering.

This significant speed difference combined with the superior sensitivity of high end CCDs has made spinning disk confocal a must have technology for advanced live cell imaging labs.

Confocal images were analyzed and quantified with the JACoP plugin [43] of the Image J software, to obtain Pearson and Manders» correlation coefficients.

Confocal imaging via spinning disk involves scanning a field with laser light from a number of pinholes arranged in a pattern on a modified Nipkow disk.

Raman spectroscopy and confocal Raman microscopy have already proved to be essential characterization tools in many areas of advanced research, with a number of these applications extending into industry.

The system provides laser free confocal imaging with large field of view and up to 100 fps frame rate, setting a new benchmark in price / performance ratio in the field of confocal microscopy