Sentences with phrase «with culture medium»

It is encapsulated with its culture medium, which provides nutritious and pH - balancing vital organic acids and vitamins, minerals, enzymes, and amino acids.

Forty - eight hours after transfection, the media were centrifuged and the supernatant was diluted with culture medium to 100 %, 50 % and 25 % the original concentrations.

After 2 — 3 weeks, propagated cells were dissociated with 0.25 % trypsin, resuspended with culture medium, and cultured for a further 2 — 12 weeks.

None of these toxic effects were observed when the same cells were treated with culture medium from non-senescent astrocytes that had not been subjected to paraquat.7

Hang and coworkers exposed the human cells by first extracting the compounds from the paper with a culture medium then using the medium to culture the human cells for 24 hours.

Not exact matches

Entrepreneur teamed up with CultureIQ to rank high - performance cultures existing at medium - sized companies for our Top Company Culturcultures existing at medium - sized companies for our Top Company CulturesCultures list.

Some reports, such as the Open Philanthropy Project's Animal Product Alternatives report and Van der Weele & Tramper (2014) suggest it is unlikely that cultured meats will become cost - competitive with conventional meat.98, 99 One important contributing factor in this conclusion, which is cited in these reports, is the minimum costs of the growth medium necessary for culturing the desired cells.

What I'd like us all to take from «wm» is a conviction that conservative pop culture studies have to attend to the impact of the medium as well as of the message, or better said, since McLuhan can't be fully right, that we must grapple with the popularity as well as the culture of pop culture.

The message behind the «culture creep» promoted by the Times is a «gospel,» says veteran reporter Proctor, because it is adhered to with religious - like devotion both in the editorial and news sections of that influential medium.

Not only must one view the individual patient as an operating biological organism, one must also seek to understand both the environing medium for that person, which includes all other persons with whom functional activity occurs, and the specific culture that to a large extent shapes the perceptual patterns by which that individual experiences the world.

With some entailment of that danger always implicit in superlatives, one may raise the question whether any other single contribution from whatever source since human culture emerged from the stone ages has had the far - reaching effect upon history that Israel in this regard has exerted both through the mediums of Christianity and Islam and directly through the world of Jewish thinkers themselves.

So it is a matter of plain fact that Christianity molded what came to be called Europe (whose original name, after all, was «Christendom»), but to say so does not by itself tell us whether that shaping of European culture through the medium of Christian ideas was a good thing or a bad thing to begin with, let alone whether those ideas speak to us now.

Ghee rendered from cultured butter is Chef Tory Miller's frying medium of choice for the fingerling potato chips that go with his beef tartare: «The chips get super crispy, with that buttery funkiness,» says Miller, the chef at Graze, an eclectic gastropub in Madison, Wisconsin.

Kombucha, which many of you are likely familiar with, differs from water kefir because kombucha is specifically cultured in a tea and sugar medium, and water kefir can be cultured using just water and sugar.

The fertilised egg (zygote) cultured for 2 - 6 days in a growth medium and is then transferred to the mother's uterus with the intention of establishing a successful pregnancy.

Unable to see the organism that causes rabies with the microscopes available, or to culture it in an artificial medium, he nonetheless convinced a -LSB-...]

When they put human macrophages in the lower compartment filled with cell culture medium, the cells rose toward the membrane.

Embryo growth after 12 months of dry storage was negligible and was observed only in embryo cultures with growth hormones (c. 12 %) and no embryo growth was evident after 3 months of storage at − 18 °C in either medium.

Cultures were maintained in complete medium consisting of Neurobasal medium (Invitrogen, Carlsbad, CA) supplemented with B - 27 (Invitrogen), 1 % FBS (Hyclone, Logan, UT), 0.4 mm l - glutamine (Invitrogen), 2.5 gm / l glucose, and 10 ng / ml 2.5 S nerve growth factor (Becton Dickinson, Bedford, MA).

The growth potential of excised embryos cultured on 1/2 MS medium declined by 31 % after 1 month of dry storage or after 24 h of dry storage at − 18 °C (Table 3), but growth was similar to that of embryos from freshly collected seeds (c. 80 %) when embryos were cultured with growth hormones.

Isolated CSCs were cultured in DMEM / F12 medium with 2 % B27, 20 ng / mL EGF, 4 μg / mL insulin, and 0.4 % of bovine serum albumin (BSA).

Spleen cells (3 × 107) from either experimental or control effector lymphocytes were dispensed into 75 - cm2 tissue culture flasks along with 20 ml of boosting medium and 6 × 105 γ - irradiated (3,000 rad) BALB / c spleen cells (erythrocytes lysed).

Human iPS cell - derived hepatocytes differentiated with our robust differentiation protocol and cultured using our novel maintenance medium provide an inexhaustible, consistent supply of functional hepatocytes that can be used to advance the understanding of diseases related to dysfunction in liver metabolism, including NAFLD / NASH, type 2 diabetes, and metabolic syndrome.

The culture medium was collected after 24 hr of conditioning and albumin content was analyzed with the Albuwell kit (Exocell) according to the manufacturer's protocol.

Human brain vascular pericytes (BP) were purchased from ScienCell (# 1200) and cultured in pericyte medium (ScienCell, # 1201) supplemented with 2 % FCS, 1 % of the corresponding pericyte growth supplement and 1 % penicillin / streptomycin.

Human iPS cell - derived hepatocytes differentiated with our robust differentiation protocol and cultured using a novel maintenance medium provide an inexhaustible, consistent supply of functional hepatocytes that can be used to advance the understanding of diseases related to dysfunction in liver metabolism, including NAFLD / NASH, type 2 diabetes, and metabolic syndrome.

Daily medium exchanges help alleviate cellular stresses associated with pH changes and resource consumption due to the high cell density of the culture.

HUVEC were purchased from PromoCell (C - 12203) and cultured in Endopan 3 medium (PAN Biotech, #P04 -0010 k)-RRB- supplemented with 3 % FCS, the corresponding supplement mix and 1 % penicillin / streptomycin.

Dermal fibroblasts were purchased from PromoCell (C - 12300) and cultured in Dulbecco's modified Eagle medium (DMEM)(Gibco) supplemented with 10 % FCS and % penicillin / streptomycin.

Then, cells were either stimulated with methionine - choline deficient media (MCD - media; WME without choline, methionine, or glutamine and with 0.1 % BSA) for 24 hr or cultured in control medium (WME with 0.1 % BSA).

Delaney et al. [4] who have shown that cord blood - derived CD34 + cells cultured in StemSpan ™ medium with cytokines, on plates coated with Notch ligand, are expanded ex vivo while still maintaining their ability to engraft into immunocompromised mice.

MDA - MB 231 and BCM2 cell lines were cultured in minimum essential medium (MEM)(Invitrogen Corporation, Carlsbad, CA, USA) supplemented with 10 % fetal bovine serum (HyClone, Logan, UT, USA; Thermo Fisher Scientific Inc., Waltham, MA, USA), 2 mM L - glutamine, 1 mM sodium pyruvate, 1 mM non-essential amino acids, and 1 % vitamin (HyClone).

One - half of the culture medium was replaced with a fresh medium every 3 — 4 days for the 16 DIV.

By culturing cells in a fed - batch system with StemSpan ™ medium containing cytokines and UM171, expanded cells were found to successfully engraft and repopulate immunocompromised mice, with no disadvantage when compared to unmanipulated cells.

Vero E6 tissue cultures [obtained from The American Type Culture Collection (ATCC), CRL: 1586] were grown in Dulbecco's modified minimum essential medium (DMEM) supplemented with penicillin (100 units / ml), streptomycin (100 µg / ml), 0.2 % sodium bicarbonate and 10 % fetal bovine serum (FBS).

To determine the effect of the conditioned medium on NGF - induced neurite outgrowth, PC12 cells were dissociated and plated on polyornithine - coated tissue culture dishes in the following conditions: 1) HT22 conditioned medium, 2) J147 treated HT22 conditioned medium, 3) DMEM alone plus J147, 4) DMEM plus NGF at 50 nanograms per ml, 5) J147 treated HT22 conditioned medium pre-incubated for one hour with 10 μg / ml anti-NGF and N2 supplement (Invitrogen).

Bone marrow cells cultured with conditioned medium derived from ABL1 / ABL2 knockdown 1833 and SKBR3 breast cancer cells had decreased numbers of TRAP + cells compared to the control groups (Fig. 5, B and C, and fig.

Culturing wild - type brains with the fat bodies from fed gbp1, gbp2 ex67 larvae showed high levels of ILP2 and ILP5 accumulation that were indistinguishable from those from brains cultured in medium alone or with starved fat bodies.

The culture and manipulation of GEMM - ESC clones is performed entirely under feeder - and serum - free conditions using the defined N2B27 medium with LIF and the two inhibitors (2i), CHIR99021 and PD0325901, as originally described by the group of Austin Smith, Cambridge, UK.

Briefly, NHLF cells were grown in 96 - well CoStar tissue culture plates (4,000 cells per well) and either subjected to siRNA gene silencing or directly treated with compounds for 51hours in 100 µL complete medium per well.

and cultured in 5 % O2 / 7 % CO2 in Bottenstein - Sato F12 medium with 10 ng / ml human recombinant basic fibroblast growth factor (Peprotech) on a substrate of 1 µg / cm2 fibronectin (Chemicon) and 0.5 µg / cm2 laminin (Invitrogen).

The human breast cancer cell line SKBR3 was purchased from the Duke University Cell Culture Facility and was maintained in McCoy's 5A medium (Life Technologies) supplemented with 10 % FBS (Life Technologies) and antibiotics.

For example, Masato Kanemaki's lab has developed a CRISPR / Cas - based system for tagging endogenous proteins with an auxin - inducible degron (AID) tag to generate proteins which can be rapidly and reversibly degraded after the addition of auxin to the culture medium.

It is also worthy to mention that human ES cells and iPS cells could be maintained on the HFF cells with the conventional human ES culture medium (Li et al, unpublished data).

We standardized the densities of ILPs by fixing the values from the «no fat body» treatment (culturing with a plain medium) to 1.

Before staining, the medium was replaced with serum - free medium and cultured overnight.

SH - SY5Y cells were cultured in Dulbecco's modified Eagle's medium nutrient mixture F - 12 (DMEM / F12; Sigma), supplemented with 10 % FBS (Sigma), 100 nM l - glutamine (Sigma), 100 U / ml penicillin (Sigma), 10 µg / ml streptomycin (Sigma), in a humidified atmosphere containing 5 % CO2 at 37 °C.

A single - cell suspension (50 μl) containing 1500 cells was mixed with Matrigel (1:1) and plated on top of the Matrigel base onto wells of a 96 - well plate; 50 μl of complete medium was added, and the cells were cultured for 14 days.

The colonies were cultured in suspension in Petri dishes with ES cell medium [14], and the culture medium was changed every 3 days.

After three weeks, all medium from the six - well cultures was removed, followed by a single media wash and replacement with fresh medium for 24 hours.