Sentences with phrase «with cytoplasmic»

Interferon regulatory factor subcellular localization is determined by a bipartite nuclear localization signal in the DNA - binding domain and interaction with cytoplasmic retention factors

Human iPS - RPE are pigmented cuboidal epithelial cells with cytoplasmic polarization.

Importantly, spreading and migration do not occur with cells expressing NG2 variants lacking the cytoplasmic domain of the proteoglycan, suggesting that interaction of NG2 with cytoplasmic ligands is required for these processes to take place.

The group first transduced a CD4ζ construct (CD4 extracellular and transmembrane domains fused with cytoplasmic domain of TCR CD3ζ chain linked to GFP — See Figure) into hESCs and hiPSCs using the Sleeping Beauty (SB) transposon system and positive clones selected for by antibiotic resistance, giving clones with strong CD4ζ - GFP expression (37).

Quantitative analysis of endocytosis with cytoplasmic pHluorin chimeras.

Not exact matches

Four cytoplasmic regions form a gating ring at the intracellular membrane surface with four Ca2 + binding sites on the outer perimeter.

They found that the N - terminal end of the cytoplasmic tail of gp41, measuring 45 amino acid residues, lacked a regular secondary structure and was not associated with the membrane, which wraps around an HIV - 1 viral particle like the leather cover of a baseball.

The C - terminal end of the cytoplasmic tail of gp41, measuring 105 amino acid residues, was tightly associated to the membrane and had three alpha - helixes with portions that were hydrophobic and portions that were hydrophilic.

The PERY peptide specifically blocked the interaction of p38 and JNK with the importins, restricted their nuclear translocation, and inhibited phosphorylation of their nuclear (but not cytoplasmic) substrates.

In order to keep step with the «quickened» mitochondrial translation, somatic cells activated the mTOR signaling pathway in order to accelerate cytoplasmic translation and balance mitochondrial translation.

The assembly includes the cytoplasmic (T1) domain of the integral membrane α subunit together with the oxidoreductase β subunit in a fourfold symmetric T14β4 complex.

Our work has shown that it all begins with the oocyte, which can have subtle cytoplasmic defects that are actually very profound.

«It's kind of like a lava lamp,» he says, «with these blobs of cytoplasmic elements moving up and down in the cell.»

For instance, by ensuring that infected males can't reproduce with uninfected females — a phenomenon called cytoplasmic incompatibility — the bacteria can maximize the number of infected offspring in the next generation and sweep through populations in very little time.

As full length Bk132 Env would not pseudotype on an NL43 HIV core we truncated the cytoplasmic tail of the Envs [49], [50], and conducted tropism testing on NP2 cell lines expressing CD4 with either CCR5 or CXCR4.

The translational fusion constructs resulted in high levels of cytoplasmic proteins present in intestinal and epidermal cells on vesicular structures with the characteristic appearance of lipid droplets.

As seen in Figure 2, the abnormal BM cells were found to exhibit eccentric, large, round - to - oval / convoluted nuclei with mostly fine chromatin and prominent nucleoli and moderate - to - abundant gray cytoplasm with several cells exhibiting multiple diminutive cytoplasmic vacuoles.

The control spot shows an even distribution of cytoplasmic p65 with a single nuclei in the center of cells.

281.2 Human CD4 with deletion of cytoplasmic domain, produces CD4 molecule truncated at aa394.

We investigated the role of the cytoplasmic tyrosine kinase JAK2 in patients with a myeloproliferative disorder.

This result was agreed with several previous studies who stated that, Cyclin D1 showed nuclear and cytoplasmic staining and had an extensive range of intensities between different sections [22, 23].

An adult cell from the mammary gland of a Finn - Dorset ewe acted as the nuclear donor; it was fused with an enucleated egg from a Scottish Blackface ewe, which acted as the cytoplasmic (or egg) donor.

Consistent with a decrease in E-cadherin expression, MDA - MB 231 F3 and BCM2 F3 cells had increased nuclear expression of Snail and cytoplasmic expression of a mesenchymal marker vimentin (Figure 6c).

As outlined below, we used a microfluidic quantitative PCR (qPCR) system to elucidate the gene expression profiles of individual human oocytes and small numbers of cumulus cells using a combination of a large number of samples and targets [12], and then extended our studies via the use of parthenogenesis, in conjunction with gene expression profiling, as a functional assay of cytoplasmic maturation of oocytes.

Cell type - specific integrin variants with alternative alpha chain cytoplasmic domains.

In fibrosarcoma and neuroblastoma cells, huntingtin shows discrete punctate, perinuclear localization overlapping largely with the trans - Golgi and cytoplasmic clathrin - coated vesicles, implicating huntingtin in vesicle trafficking.

Autophagy describes how cells replace themselves and their components over time; in scientific language, it is the process whereby cytoplasmic components are isolated from the rest of the cell within autophagosomes, which are then fused with lysosomes and degraded.

Beads coated with Arp2 / 3 - activating proteins can induce two distinct types of actin organization in cytoplasmic extracts: (1) comet tails or clouds displaying a dendritic array of actin filaments and (2) stars with filament bundles radiating from the bead.

This study addresses the requirements of each of the four CPEBs for mitotic phase transitions, with a particular focus on cytoplasmic polyadenylation and translational regulation.

With Cell Painting, a technique developed at the Broad, researchers tag eight cellular components and organelles (actin, cytoplasmic RNA, endoplasmic reticulum, Golgi apparatus, mitochondria, nucleus, nucleolus, and the plasma membrane) with fluorescent dWith Cell Painting, a technique developed at the Broad, researchers tag eight cellular components and organelles (actin, cytoplasmic RNA, endoplasmic reticulum, Golgi apparatus, mitochondria, nucleus, nucleolus, and the plasma membrane) with fluorescent dwith fluorescent dyes.

Although the cyst structure is not present in mammalian spermatogenesis, descendant cells from a single spermatogonial stem cell remain connected with each other through cytoplasmic bridges during mouse sperm development, similar to that in Drosophila spermatogenesis [62]--[65].

When we further characterized the cell lines derived from the iPSC - like colonies, we observed that they possessed hESC - like morphology, growing as flat colonies with large nucleo - cytoplasmic ratios, defined borders and prominent nucleoli (Figure 4C).

Nuclear extracts were isolated from harvested whole kidneys using NE - PER Nuclear and Cytoplasmic Extraction Reagents supplemented with Complete Protease Inhibitor Cocktail Tablets.

Skin and hair melanins are formed in cytoplasmic organelles called melanosomes, produced by the melanocytes, and are the product of a complex biochemical pathway (melanogenesis) with tyrosinase being the rate - limiting enzyme.31

Parietal cells have large mitochondria with short microvilli and a cytoplasmic canaliculi system in contact with the lumen.