Sentences with phrase «with deep sequencing»

Infant T cell repertoire analysis combines quantitative PCR to determine relative frequency of T cell receptor chain families with deep sequencing (LINK TO URI GSC) of the CDR3 region to evaluate clonal diversity within each family.

Not exact matches

How far this creativity can go in creating the human level in the case of any one individual depends partly upon his innate capacity but most of all upon two other features: (1) how wide and deep is the volume of history that reaches him, that is, how abundant and coherent are the values that have been accumulated in the history he inherits and (2) how deep is the communion he is able to have with other persons who embody these meanings accumulated through a long sequence of generations.

On the Thursday before their mid-September game against Minnesota, the Colts prepared for that very situation with this three - play sequence, as narrated by Arians: «Deep in, timeout.

Each sleep cycle is a sequence of sleep stages, beginning with relatively brief, light stages of sleep, progressing through stages of deep sleep, and ending with REM (rapid - eye movement) sleep, the sleep state associated with dreams.

Age - related variations in DNA methylation were analyzed by deep sequencing technology, which revealed distinct patterns, with gains in methylation in older individuals occurring at genomic sites that were unmethylated in young individuals, and vice versa.

«This is a story that couldn't have happened five years ago, but with the evolution of deep sequencing, we were able to use the technology at just the right time and harness it with what we've been working on for decades,» he says.

But the benefits of this technology for discerning the circuits of the mind go much deeper, because the virus that carries the photoreceptor genes can also carry promoter sequences that express their payload only in neurons with the appropriate molecular address.

That began to change last year with the discovery of DNA sequences for an organism that no one has ever actually seen living near a deep - sea vent on the ocean floor.

Understanding how a particular DNA sequence gives rise to a particular protein provides us with some insight into that protein, but a deeper investigation of how the protein is made, where it is located, and how much of it is present in different cell types is required to enable a true understanding of its function.

In light of these findings and since tRNAs are repeat sequences themselves, the team had to devise a complex and stringent scheme with which they mapped the deep sequencing reads.

Piero Carninci at the RIKEN Yokohama Institute's Omics Science Center in Japan, and colleagues, combined powerful computer modelling with «deep sequencing» — a process which decodes huge numbers of DNA sequences to discover...

Excavation revealed a deep sequence of deposits containing the elephant remains, along with numerous flint tools and a range of other species such as; wild aurochs, extinct forms of rhinoceros and lion, Barbary macaque, beaver, rabbit, various forms of vole and shrew, and a diverse assemblage of snails.

The technique, called ultra-rapid deep sequencing, combines deep sequencing — an emerging technology that reconstructs an entire DNA sequence from a tiny snippet of DNA — with advanced computational techniques and algorithms developed in the laboratories of Chiu and his research collaborators.

«Our combined analysis of cell - free DNA and white blood cell DNA allows for identification of tumor DNA with much higher sensitivity, and deep sequencing also helps us find those rare tumor DNA fragments.»

Deep sequencing analysis has shown that this is highly unlikely in the case of J3 as no sequences were generated by deep sequencing of the seven naïve llamas or three other immunized llamas that share greater identity with J3 than its putative germ line V gDeep sequencing analysis has shown that this is highly unlikely in the case of J3 as no sequences were generated by deep sequencing of the seven naïve llamas or three other immunized llamas that share greater identity with J3 than its putative germ line V gdeep sequencing of the seven naïve llamas or three other immunized llamas that share greater identity with J3 than its putative germ line V gene.

In contrast, the deep sequencing analysis of VHH present at t = 54 shows no over-representation of the sequences which share higher levels of identity with the neutralizing VHH than the germ line (Fig. 6).

(B) The most common mutations induced by the X4 - ZFNs, as detected by 454 deep sequencing, are indicated with their frequencies among all ZFN - induced lesions.

To assess low frequency disruption events, we conducted 454 deep sequencing on all 15 sites in both control CD4 + T cells and those treated with X4 - ZFNs, yielding approximately 7,500 — 26,000 reads per site in the ZFN - treated samples (Table S2).

We deep - sequence longitudinal samples from four immunocompromised patients with long - term H3N2 influenza infections.

These individuals had already been deep sequenced with Illumina technology, both for variant calling and estimation of DNA methylation based on treatment with bisulphite.

Chi's presentation focused on first - line treatment outcomes and correlations with deep targeted sequencing of circulating tumor (ctDNA) that occurred at baseline.

To find a solution to this, Marcel Martin at Bioinformatics Long - term Support (WABI) at SciLifeLab, in collaboration with the group of Gunilla Karlsson Hedestam at Karolinska Institutet, has developed a practical, computational technique, IgDiscover, that enables rapid production of individualized V gene databases following deep sequencing of expressed antibody repertoires.

Deep sequencing was performed with established Illumina protocols on the GAIIx and MiSeq platform (Illumina, California, USA).

A consensus sequence based on mapped deep sequencing reads was generated for each of the prostate cancer XMRV genomes and used to correct errors in the previously published sequences, with the requirement of no ambiguity at each discrepant nucleotide position.

Both of these cell line - associated XMRV genomes were found to exhibit a lower degree of intra-strain variation than previously reported for XMRV from 22Rv1 cells [20], with only 19 SNPs detected in the 22Rv1 - associated XMRV genome at the 3 % frequency cutoff by deep sequencing, and only 25 SNPs in the LNCaP - associated genome (Fig. 7A; Table S1).

To examine the micro-evolution of S. aureus colonization we have deep - sequenced S. aureus populations from nine children with moderate to severe AE, and 18 non-atopic children asymptomatically carrying S. aureus nasally.

Importantly, while the RNA from sample VP62 extracted in 2006 was positive for XMRV, RNA extracted from the same prostate tissue in 2012 was found to be negative not only by ViroChip and PCR (Fig. 2; Table 1) but also by deep sequencing, with no XMRV sequences detected out of 4 million deep sequencing reads (Fig. 5).

We then expanded the project to the other two sites, from which we screened many more samples than we could sequence deeply, so we selected the best four samples (with highest complexity and endogenous DNA) from Oakington and the best from Linton (from which we had fewer samples, and there was only one sample with acceptable complexity for deep sequencing).

MyAML combines long read chemistry and deep sequencing with an optimized and validated custom bioinformatics pipeline, MyInformatics ™, to specifically examine genomic variants in AML patients.

Coinciding with CIRM's goals, three of the floors planned for renovation will accommodate the collaborative work of top stem cell researcher and UCSB professor James Thompson, who was the first to isolate human embryonic cells in 1998; the new Mellichamp and Ruth Garland Chairs of bioengineering and molecular mechanisms, respectively; and the «Deep Sequencing Core,» which will house, among other things, a device that will help develop new genetic approaches to important cell biology inquiries.

Deep sequencing of cerebral white matter - derived RNA from the HIV (n = 4) and ODC (n = 4) patients and SURG (n = 2) groups revealed bacterially - encoded 16 s RNA sequences in all brain specimens with α - proteobacteria representing over 70 % of bacterial sequences while the other 30 % of bacterial classes varied widely.

Held in a open, architecturally - designed space heated to 37 degrees — the temperature thought to dissolve the sensation of separateness from one's surroundings — each 60 minute class begins and ends with breathing exercises known as pranayama, gradually adding depth and complexity with each sequence and progressing to deeper stretching and strengthening in the energizing standing series.

For example, by deep sequencing bacterial DNAs from diseased and healthy subjects, the inventors propose that features associated with certain microbiome compositional and / or functional features (e.g., the amount of certain bacteria and / or bacterial sequences corresponding to certain genetic pathways) can be used to predict the presence or absence of an autoimmune condition.

This powerful and invigorating flow will combine the fluid movements of Vinyasa with deeper holds, innovative sequencing, advanced postures and inversions.

Each inhale and exhale during the practice of Ashtanga Yoga's sequences of postures is performed with a deep, resonant sound created at the back of the throat.

Warm up with a flowing sequence of repetitive hip openers, deepen your focus using a block with long - held poses and explore the deep release benefits of pigeon pose.

Fold & Flow with Anna — Take time to connect with breath, body and mind in a guided hatha (meaning simply physical) yoga practice that will include gentle awakening stretches, deeper (yin style) holds, connecting breath with movement, and a creative vinyasa (flowing) sequence, always finishing with relaxation.

Vigorous morning yoga intensives guide you deeper into your own practice and life purpose, while afternoon instructional sessions cover an extensive yoga teacher curriculum, including cueing, sequencing, hands - on assists, working with injuries, demonstration techniques, vocal presence, yoga philosophy and history, the business of yoga, ethics, and anatomy.

Utilizing pranayama, chant, and creative and intelligent sequencing, Tracy encourages students of all levels to practice intuitively and with deep respect for their individual yogic paths.

This sequence is designed to slowly warm up with breath and continuous movement; and then go deeper into energizing postures that will leave you feeling ready to head out into the brisk air.

However, Hittman composes quieter sequences with evocative images that cut deep into Frankie's hidden longing and desire.

Speaking of Bisset, many filmgoers will remember The Deep primarily for that lengthy opening dive sequence and the following scene where she dries off in a boat with co-star Nick Nolte.

Monochrome sequences offer a nicely graduated gray scale with very good contrast and blacks that are acceptably deep.

Dwayne Johnson is protagonist Ray, a hotshot helicopter pilot with the L.A. fire department who demos his bona fides in the opening sequence, involving the rescue of a blonde in a low - cut top whose car is jammed into the rock wall on one side of a deep chasm.

A 2.35:1, anamorphically - enhanced widescreen video transfer, after a few line flaws on the negative during the opening credit sequence, is remarkably clear and saturated with the deep greens of Argento's moody colour palette.

Tarsem Singh, a director known for his astonishing visuals more than his deep storytelling or compelling characters, continues his usual style by making an undoubtedly nice - looking flick but one which has only intermittent fun action sequences with mind - numbingly boring scenes in between.

Morris starts with a covert meeting in Deep Creek Lake, Maryland, where Frank's brandy was laced with LSD, and ends with Frank's plunge out of the hotel window, revisiting this week and a half over and over again, revising, adding, and subtracting a panoply of moments and sequences.

«While the themes are deep, Black Panther is at the same time a visual joy to behold, with confident quirkiness (those aforementioned war rhinos), insane action sequences and special effects, and the glorious reveal of Wakanda, whose culture is steeped in African influences but which also offers a jaw - dropping look at what a city of the future could be... Let's not wait too long for a return trip.»

Miller's message is clear: if you need an action film with some deep, intricate plot to make the crazy death - defying sequences work for you, «Fury Road» is definitely not your kind movie.

Sadly, the problem with the film lies deeper than these fleeting yet exhilaratingly technical sequences, as events cumulate into repetitive and predictable misadventures.