Sentences with phrase «with diaminobenzidine»

Not exact matches

Immunoreactions were developed using a fluorescently labeled secondary antibody, and samples visualized in a Leica Biosystems epifluorescent microscope or using a secondary antibody linked to horseradish peroxidase and developed with a 3,3 ′ - diaminobenzidine kit.

Stainings were developed with 0.06 % 3,3 ′ - diaminobenzidine (Sigma - Aldrich, USA) and 0.01 % hydrogen peroxide (Sigma - Aldrich, USA) in PBS and then mounted on subbed slides.

Antibodies are labeled with DAB (3,3» - diaminobenzidine) and the resulting brown staining indicates where an antibody has bound to its corresponding antigen.

Slides were then incubated with Envision secondary followed by 3,3 ′ - diaminobenzidine.