The focus is on protein detection in live versus fixed cells: determination of protein expression, localization, activity state, and the possibility for combination of fluorescent light
microscopy with electron microscopy.
The authors of this study combined live cell
imaging with electron microscopy to observe Trichoplax feeding behavior at scales ranging from the whole animal to subcellular.
«We examined the
samples with electron microscopy using thin layers cut out of the composite with ion beams,» explains Tobias Kraus, Division Head for Structure Formation at INM.
Co-author Lisa Tell summarizes: «This study was exciting because not only were we able to document the presence of a mite on feathers from two species of hummingbirds found in California, but we were also able to examine the positioning of live feather mites in
situ with electron microscopy that is portable enough to use in the field.»
She and her research group recently demonstrated CLAIRE's imaging capabilities by applying the technique to aluminum nanostructures and polymer films that could not have been directly
imaged with electron microscopy.
Korenberg's team is developing a 3D coordinate system to align various types of neuroimaging data in the macaque brain, from whole - brain MRI connectivity to single - cell confocal data and, for some areas, subcellular
resolution with electron microscopy.
Inside the ALH84001 carbonates, McKay spotted odd features that resembled very small worm - like fossils, so he asked Thomas - Keprta to look at them more
closely with electron microscopy.
In depth biochemical investigations
coupled with electron microscopy have shown that at this threshold the balance between safety and toxicity of copper becomes very unstable and liver damage, involving a process known as oxidant stress, becomes very likely.
The major individual particle types
observed with electron microscopy analysis (TEM / EDX) were mainly related to residential wood combustion (K / S / C - rich, soot, other C - rich particles), traffic (soot, Si / Al - rich, Fe - rich), heavy fuel oil combustion in heat plants or ships (S with V - Ni - Fe), LRT pollutants (S / C - rich secondary particles) and sea salt (Na / Cl - rich).
A new technique enables 3 - D visualization of chromatin (DNA plus associated proteins) structure and organization within a cell nucleus (purple, bottom left) by painting the chromatin with a metal cast and imaging
it with electron microscopy (EM).