Sentences with phrase «with fluorescent labels»

Researchers at SciLifeLab / Uppsala University headed by Johan Elf have used a combination of microfluidics and image analysis of E. coli with fluorescent labels on the replication machinery to study how the replication and division cycles are coupled in individual cells.

Human induced pluripotent stem cell neurons imaged in phase contrast (gray pixels, left)-- currently processed manually with fluorescent labels (color pixels) to make them visible.

«We trained the neural network by showing it two sets of matching images of the same cells; one unlabeled and one with fluorescent labels,» coauthor Eric Christiansen, a software engineer at Google Accelerated Science, says a press release.

«We trained the neural network by showing it two sets of matching images of the same cells; one unlabeled and one with fluorescent labels,» explained Christiansen, software engineer at Google Accelerated Science.

Machines copy DNA strands and modify them with fluorescent labels and other compounds to enable them to read DNA's sequence letters, or bases.

Not exact matches

While light - sheet microscopy is an old idea — scientists at ZEISS Microscopy and collaborators first came up with it in 1903 — only in this century has the convergence of fluorescent labels that work to process image volumes combined to make light - sheet mainstream.

Traditional techniques involve labelling or injecting the cells with stains or fluorophores — fluorescent compounds that «glow» when exposed to light — to detect their intricate structures.

The system uses an enzyme called DNA polymerase to read a strand of DNA and build a complementary strand out of nucleotides labeled with fluorescent molecules.

Blood stem cells in the bone marrow have been labeled with a fluorescent marker protein.

Developmental biologist Francesca Peri of the European Molecular Biology Laboratory in Heidelberg, Germany, and colleagues created genetically engineered versions of the animals that produced microglia labelled with green fluorescent protein, a glowing compound frequently used in laboratory research.

To image glucose uptake activity at the cellular level, glucose analogues labeled with fluorescent dyes have been developed.

In order to test the sensitivity and specificity of spectral histopathology, the RUB team compared SHP with classical immunohistochemical methods, in which tumours are identified with the aid of fluorescent labels.

One side of each dumbbell was filled with a different short sequence of DNA labeled with a fluorescent molecule.

The group employed various viral tracing methods — infecting receptor - expressing neurons with a virus strain and watching them spread as they label infected cells with a fluorescent protein — to visualize the neural circuit downstream of the ESP1 receptor, as well as providing an image of nerve fibers belonging to specific neurons in the brain and synapses relaying impulses from neuron to neuron, to map the anatomical foundation that conveys ESP1 signals in the brain.

Typically, detection of biomolecules such as proteins are performed using colorimetric assays or fluorescent labelling with a secondary antibody for detection, and requires complex optical detection equipment such as fluorescent microscopy or spectrophotometry.

Using new technologies for labeling cellular machinery with light - activated fluorescent markers, they could «turn on» just one molecule at a time.

In the Nature Biotechnology study, the researchers used it to anchor proteins, and they also showed that the technique works on tissue that has been previously labeled with either fluorescent antibodies or proteins such as green fluorescent protein (GFP).

Such vectors have become critical tools for delivering genes to treat disease or to label neurons and their connective fibers with fluorescent colors to map out their locations.

Smith's team labelled the virus with green fluorescent protein, and labelled some — but not all — cells with a red marker that tagged the actin.

An early skate embryo that has just been labeled with the fluorescent dye.

The group of imaging specialists led by Prof. Michael Schäfers, Coordinator of the Cluster of Excellence, labelled the cells thus obtained with various fluorescent dyes in order to be able to study them in living organisms — initially with the optical method of fluorescence reflectance imaging.

Instead, the new method, called patterned photoactivation non-linear SIM, begins by switching on just a subset of fluorescent labels in a sample with a pattern of light.

By labeling the different subgroups with fluorescent dyes, researchers determined that just one of the subclones was responsible for the disease metastasis.

IEC - 6 cells were labeled with either red or green fluorescent dyes.

Wellington and her colleagues detected the dormant cells with fluorescent - labelled antibodies which cling specifically to S. typhimurium.

A thalamocortical, or TC neuron labeled with fluorescent dye, as used in Dr. Augustinaite's study.

They removed spermatogonia labeled with a fluorescent marker from a mature male trout and injected the cells into the abdomens of newly hatched male and female trout fry.

Sandia National Laboratories researchers are drawing inspiration from neurons in the brain, such as these green fluorescent protein - labeled neurons in a mouse neocortex, with the aim of developing neuro - inspired computing systems to reboot computing.

Then they injected the mice with lymphocytes labeled with a fluorescent dye.

Nine weeks after receiving the injections, long after the animal was born, the scientists examined its brain to see where the cells, which they'd labeled with a fluorescent marker, had gone.

Specifically, cell cultures from bone tissue were incubated in 10 % DMEM containing 10 ng / ml TNF - α and 10 μg / ml fluorescent probe of acetylated LDL, DiI - Ac - LDL (Biochemical Technologies, Cambridge, MA), for 4 h. Cells were harvested in the manner described above with the exception that during this labeling period, the rat anti-E-selectin mAb (10E9.6) conjugated to FITC replaced PE - conjugated 10E9.6.

Because zebrafish are transparent until their stripes emerge in adulthood, Oehlers could label the bacteria, immune cells, and vasculature with differently colored fluorescent tags and then follow a TB infection over time.

They found that injecting into the carotid artery breast cancer cells that express markers allowing them to enter the brain — cells labelled with bioluminescent and fluorescent markers to enable tracking by imaging technologies — resulted in the formation of many metastatic tumors throughout the brain, mimicking what is seen in advanced breast cancer patients.

The injected cells express markers that allow them to enter the brain and are labelled with bioluminescent and fluorescent markers to enable tracking by imaging technologies.

By labeling one of the shell proteins with Yellow Fluorescent Protein (YFP), we were able to use confocal fluorescence microscopy to observe punctuate loci forming inside chloroplasts.

The membranes of cells that have been successfully targeted with gesicles are transiently labeled with red fluorescent protein.

One common complaint about using four - color Confetti to label single cells (in an animal) is that the cells express green fluorescent protein less often, or sometimes not at all, compared with cells that express each of the other three colors.

The ability to label different subcellular locations with the green fluorescent protein (GFP) has made it possible to visualize intracellular activities in living cells.

Furthermore, once these proteins are identified, they can be selectively labeled with antibodies linked to green fluorescent protein (GFP) and directly observed in a cell.

The authors then tested the tumor targeting capability of iPSC - EPCs labelled with the near - infrared fluorescent dye DiR in two established mouse breast cancer models.

Control and gesicle - treated hiPS cells were assessed for AcGFP1 expression (green), labeled with an antibody specific to pluripotency marker Oct ‑ 4, visualized with a fluorescent - labeled secondary antibody (red), and nuclear - labeled with DAPI (blue).

Using fluorescent labels with unlabeled images to train a deep neural network to bring out image detail.

«It tells you about the ecosystem of the tumour», Simona says, «using fluorescent markers, we can label many specific cell types and structures within the brain in the vicinity of the tumour, and look not just at the tumour cells but how they interact with their surroundings.

A cortical layer 2/3 neuron at 21 days in vitro labeled by ex utero electroporation with fluorescent marker for spine visualization.

In 2002, an offer to join the faculty of MIT's Chemistry Department drew her back to the East Coast, where she was able to begin tackling the problem that had «repeatedly stymied» her throughout her PhD and postdoc years: site - specific protein labeling — how to label a particular protein with a fluorescent tag or other «chemical probe» to reveal information about that protein's function.

(Right three images) Labeled images created with three different fluorescent labels, revealing invisible details of cell nuclei (blue), dendrites (green), and axons (red).

Neurosurgeons are partnering with chemists to develop fluorescent «labels» that can pinpoint the location of cancerous cells in the brain.

For example, with standard fluorescent microscope filter sets, users can label multiple proteins of interest with a combination of green and blue or cyan and yellow moxFPs.

Close - up of muscle fibres in a zebrafish with the protein, actin, labelled with green fluorescent protein (GFP) Image credit: Elisabeth Busch, Wellcome Trust Sanger Institute

However, we recently developed AAV (adeno - associated virus)- based fluorescent labeling methods that can be used with 3DISCO to image non-transgenic animals such as rats (17).