Sentences with phrase «with fluorophores»

In order to specifically label molecules with fluorophores in intact cells, we prepare highly stable unnatural amino acids that rapidly and irreversibly undergo cycloaddition reactions (click chemistry) with unsurpassed speed and study their application in collaboration with the Lemke group.

Fuat Aksoy (Pompe, TUD)-- «Interaction of metal nanoparticles with fluorophores and their effect of fluorescence» (2009)

Single cell suspensions were washed in PBS and then stained for 20 — 30 min on ice in the dark with fluorophore - labeled Abs (for a complete list see Tables 1 and 2) and fixable viability dye (eBioscience).

Not exact matches

In their latest work, Still's team strung these molecules, called chemosensors, with a molecule called a fluorophore that glows when the sensor alters its shape after binding to a peptide.

Traditional techniques involve labelling or injecting the cells with stains or fluorophores — fluorescent compounds that «glow» when exposed to light — to detect their intricate structures.

Researchers at Osaka University overcame these limitations with fluorescent SM analogs by joining several fluorescent chemical compounds (fluorophores) that were highly hydrophilic to the hydrophobic lipid part (mainly acyl chains) of the synthetic molecule.

Recombinant proteins containing tetracysteine tags can be successively labeled in living cells with different colors of biarsenical fluorophores so that older and younger protein molecules can be sharply distinguished by both fluorescence and electron microscopy.

Instead, they create a library of several hundred fluorophores with varying chemical additions and three - dimensional conformations and then test each in mice to see which are taken up by specific tissues in the body.

The fluoresence image (b) shows a 4 - layer lattice printed by sequentially depositing four PDMS inks (c), each dyed with a different fluorophore.

This fluoresence image shows a 4 - layer lattice printed by sequentially depositing four PDMS inks, each dyed with a different fluorophore.

Its emission varies depending on pH levels, the blue violet has an acidic pH and yellow a neutral pH. Nuclear green is a nuclear - specific DNA dye that simplifies working with red fluorophores.

(b) Proteins in contact with a bifunctional lipid are tagged in vivo by photoaffinity labeling and then derivatized in vitro with biotin or a fluorophore using click chemistry, allowing their purification or visualization.

The brightest fluorophore should be reserved for the detection of the antigen with the least abundant expression level.

Use fluorophores with a narrow emission spectrum in multi-color IF experiments to avoid spectral overlap

- Based on the expression level / pattern of the protein of interest, decide what antigen to detect with which fluorophore in multi-color experiments.

In Figure 4, Panel B, cells were incubated simultaneously with anti ‑ SSEA ‑ 4 and anti - TRA ‑ 1 ‑ 60 antibodies, since they were labeled with different fluorophores (PE and FITC, respectively).

For certain filters, e.g., FITC / TRITC filters, the compatibility evaluation is quite easy as they have been named after the fluorophores they are meant to be used with.

A Fluorophore Fusion - construct of Human Profilin I with Non-compromized poly (L - Proline) Binding capacity suitable for Imaging.

Alternatively, anti-horseradish peroxidase conjugated with other enzymes or fluorophores may be used to enhance signal and reduce background, since the final signal does not depend on the enzyme activity of peroxidase, but on the antigenicity of horseradish peroxidase.

«This core is functionalized with a dense layer of stable dithiol - modified DNA oligonucleotides hybridized with distinctive fluorophore - capped «reporter» sequences.»

The result is illumination from the microlens disk through the pinhole disk for strong excitation of fluorophores, causing a fluorescence emission which in turn passes through the pinhole disk with high confocality.

Instead, you can pre-incubate individual primary antibodies with secondary nanobodies conjugated with the desired fluorophores.

TIRF is also very gentle on the sample as the majority of the laser light is reflected away from the sample and excitation occurs through the fluorophore's interaction with an evanescent energy field.

Following incubation for 60 min at 37 °C, the slides were washed extensively with PBS and secondary antibodies conjugated to fluorophores were added for 30 min.

Experts with extensive experience using FRET discuss the practical considerations that need to be considered when embarking on a study using FRET, for example, selection of fluorophore pairs and minimisation of imaging - induced artefacts.

CEP8 - SA probe complementary to human chromosome 8 - specific centromeric repetitive sequences and directly labeled with SpectrumAqua fluorophore was obtained from Abbott Molecular, Inc. (Des Plaines, IL).

Homogeneous Time - Resolved Fluorescence (HTRF) Utilizing rare - earth lanthanides with long emission half - lives as donor fluorophores, HTRF technology combines standard FRET with the time - resolved measurement (TR) of fluorescence.

The Tol2 Gateway - Compatible Toolbox is based on the original Tol2kit generated by the Chi - Bin Chien lab (Kwan et al., 2007) and includes four promoters, six fluorophores with nonoverlapping emission spectra (N - and C - terminal tags for mTagBFP, TagRFPt, EGFP, mVenus, mCerulean3, mKOFP2) and empty vectors that have standard cloning sites or gateway compatible cloning sites for easy cloning of your genes of interest.