Sentences with phrase «with gene expression profiling»

As outlined below, we used a microfluidic quantitative PCR (qPCR) system to elucidate the gene expression profiles of individual human oocytes and small numbers of cumulus cells using a combination of a large number of samples and targets [12], and then extended our studies via the use of parthenogenesis, in conjunction with gene expression profiling, as a functional assay of cytoplasmic maturation of oocytes.

Conditional Activation of RET / PTC3 and BRAFV600E in Thyroid Cells Is Associated with Gene Expression Profiles that Predict a Preferential Role of BRAF in Extracellular Matrix Remodeling

Not exact matches

In a paper published in Nature Methods, postdoctoral fellows Naomi Habib, Inbal Avraham - Davidi, and Anindita Basu; core institute members Feng Zhang and Aviv Regev; and their colleagues reveal DroNc - Seq, a single - cell expression profiling technique that merges sNuc - Seq with microfluidics, allowing massively parallel measurement of gene expression in structurally - complicated tissues.

Overlaying that information onto data from gene - expression profiling characterizes the microglia with unprecedented precision.

Lead author Johan den Boon, associate scientist with the Morgridge Institute for Research at the University of Wisconsin - Madison, says the research team used gene expression profiling on 128 specimens from the Study to Understand Cervical Cancer Early Endpoints and Determinants, or SUCCEED.

The researchers identified the LSC17 score by sampling the leukemia stem cell properties of blood or bone marrow samples from 78 AML patients from the cancer centre combined with molecular profiling technology that measures gene expression.

«Our present study shows examination of the gene expression profiles at the very early age of initial clinical detection reveals both strong evidence of early biological processes in ASD and abnormal signals with the potential to serve as an early, practical biomarker of risk for the disorder in general pediatric settings.»

The case for direct interaction was bolstered by similarities between gene expression profiles of plants with various deficiencies in photoreceptors and COP1.

Oncologists welcome gene expression profiling tests as an added tool in deciding whether women with early - stage breast cancer should have chemotherapy, a new study has found.

To identify genes whose expression correlated with adiposity, we profiled gene expression in perigonadal adipose tissue from 24 mice in which adiposity varied due to sex, diet, and the obesity - related mutations agouti (Ay) and obese (Lepob).

Hormone ‐ replacement therapy influences gene expression profiles and is associated with breast ‐ cancer prognosis: a cohort study

While expression levels of all marker genes remained almost unchanged in wild - type ACVR1 cultures compared with the empty viral vector, the expression profile induced by caACVR1 Q207D displayed evidence of advanced chondrogenic differentiation as shown by decreased levels of the early differentiation stage marker collagen type II together with enhanced expression of the later - stage markers Ihh and collagen type X. Indian hedgehog has been shown previously to be induced by BMP signaling transmitted via the ACVR1 receptor (9).

In recent years DNA - and RNA - based surveys of tumor genome and expression profiling have produced a plethora of leads on genes with clinical significance.

A detailed comparison of gene expression signatures between ETP ALL tumors and and normal human hematopoietic progenitor cells revealed a somewhat surprising finding: ETP - ALL expression patterns were less consistent with early T - cell precursors, as might have been expected, but more similar to the expression profile of normal hematopoietic stem cells and granulocyte macrophage precursors.

Development of a gene expression assay in non-human primates: We will develop a novel sensitive assay for the characterisation of vaccination - related gene expression profiles in NHPs, using an approach that is fully harmonised with the systems biology platform.

This will be achieved by exposing the coral to seawater with high calcium concentrations to induce an alteration in gene expression profiles, identified using a DNA microarray analysis.

The Stylophora pistillata gene expression profile was analyzed with Partek Genomics Suite ™ software (Fig. 3A) in a principal component analysis (PCA).

We use extensive genomic and gene - expression profiling techniques to identify molecules and molecular pathways that mediate the dynamic changes observed in vitro, and which are associated with different clinical outcomes in melanoma patients.

We observed that in the majority of tissues there are many genes that display expression profiles that are more complex than simple monotonic changes with PMI.

The approach to target validation utilizes RNAi, CRISPR, and ORF expression platforms to genetically perturb candidate target gene expression in relevant cancer cell lines, and then profile the resulting phenotypic changes with regard to their effect on various biochemical pathways.

Proteomic analysis to profile protein abundance resulted in the identification and relative quantification for 912 proteins with two or more unique peptides and 86 proteins with significant abundance changes after treatment with the neurotoxins, while microarray analyses to profile gene expression revealed 181 genes with significant changes in mRNA after treatment.

HDAC inhibitors and decitabine are highly synergistic and associated with unique gene - expression and epigenetic profiles in models of DLBCL

Previous studies have linked gene expression profiles in bCSCs with a clinical outcome [1, 2], and in this study the authors have extended this out to demonstrate that a DNA methylation profile may promote these changes, and furthermore, that this profile can be effectively used to predict prognosis, and potentially aid the therapeutic decision making.

With DriverMap, Cellecta offers a novel, comprehensive end - to - end service portfolio for identifying differential gene expression, mapping of clinically actionable mutations in RNA, detecting cellular composition, and profiling immunotherapy targets.

Dr. Loftus's research has gone on to detail how SOX10 and its downstream target gene MITF coordinately regulate gene expression profiles associated with melanocyte differentiation.

Approximately 50 % of PTCL are unclassifiable and categorized as PTCL, not otherwise specified (PTCL - NOS).1 Using gene expression profiling, PTCL - NOS lymphocytes can be distinguished from normal T lymphocytes, with deregulation of genes involved in apoptosis, proliferation, cell adhesion, and transcription regulation.2 Two subgroups of PTCL - NOS have been identified, which are characterized by high expression of either GATA3 or TBX21 / T - bet transcription factors and downstream target genes.3 However, actionable biomarkers closely related to the pathogenic mechanism need to be further investigated and may become potential therapeutic targets of PTCL - NOS. 4, 5

The investigators profiled the expression of microRNAs (miRNAs), which regulate gene expression, in aqueous humor samples from patients with POAG, XFG and cataracts (non-glaucoma controls).

To better illustrate gene expression profiles in mouse ES cells, we have organized the results in an interactive database with a number of features and tools.

After clicking on any of the panels, for example on the top left panel marked with a red asterisk, the expression profiles of the individual genes that follow the corresponding pattern as well as their names appear in a new window (as shown in B).

These include: a) Global Clusters that consist of a small, tight subset of genes that are co-expressed under the entire spectrum of experimental conditions; b) Time Series of gene expression profiles during successive days of standard ES cell differentiation; c) Specific Gene Classes based on hierarchical clustering of transcriptional factors and ESTs; d) Expression Waves of genes with characteristic expression profiles during ES cell differentiation, juxtaposed to waves of genes that behave in the exact opposite way; e) Pathway Animations that illustrate dynamic changes in the components of individual KEGG signaling and metabolic pathways viewed in time - related manner; and, f) Search Engines to display the expression pattern of any transcript, or groups of transcripts, during the course of ES cell differentiation, or to query the association of candidate genes with various FunGenES database clustgene expression profiles during successive days of standard ES cell differentiation; c) Specific Gene Classes based on hierarchical clustering of transcriptional factors and ESTs; d) Expression Waves of genes with characteristic expression profiles during ES cell differentiation, juxtaposed to waves of genes that behave in the exact opposite way; e) Pathway Animations that illustrate dynamic changes in the components of individual KEGG signaling and metabolic pathways viewed in time - related manner; and, f) Search Engines to display the expression pattern of any transcript, or groups of transcripts, during the course of ES cell differentiation, or to query the association of candidate genes with various FunGenES databaseexpression profiles during successive days of standard ES cell differentiation; c) Specific Gene Classes based on hierarchical clustering of transcriptional factors and ESTs; d) Expression Waves of genes with characteristic expression profiles during ES cell differentiation, juxtaposed to waves of genes that behave in the exact opposite way; e) Pathway Animations that illustrate dynamic changes in the components of individual KEGG signaling and metabolic pathways viewed in time - related manner; and, f) Search Engines to display the expression pattern of any transcript, or groups of transcripts, during the course of ES cell differentiation, or to query the association of candidate genes with various FunGenES database clustGene Classes based on hierarchical clustering of transcriptional factors and ESTs; d) Expression Waves of genes with characteristic expression profiles during ES cell differentiation, juxtaposed to waves of genes that behave in the exact opposite way; e) Pathway Animations that illustrate dynamic changes in the components of individual KEGG signaling and metabolic pathways viewed in time - related manner; and, f) Search Engines to display the expression pattern of any transcript, or groups of transcripts, during the course of ES cell differentiation, or to query the association of candidate genes with various FunGenES databaseExpression Waves of genes with characteristic expression profiles during ES cell differentiation, juxtaposed to waves of genes that behave in the exact opposite way; e) Pathway Animations that illustrate dynamic changes in the components of individual KEGG signaling and metabolic pathways viewed in time - related manner; and, f) Search Engines to display the expression pattern of any transcript, or groups of transcripts, during the course of ES cell differentiation, or to query the association of candidate genes with various FunGenES databaseexpression profiles during ES cell differentiation, juxtaposed to waves of genes that behave in the exact opposite way; e) Pathway Animations that illustrate dynamic changes in the components of individual KEGG signaling and metabolic pathways viewed in time - related manner; and, f) Search Engines to display the expression pattern of any transcript, or groups of transcripts, during the course of ES cell differentiation, or to query the association of candidate genes with various FunGenES databaseexpression pattern of any transcript, or groups of transcripts, during the course of ES cell differentiation, or to query the association of candidate genes with various FunGenES database clusters.

Expression profiles of the 19 Wnt genes during ES cell differentiation with the corresponding Affymetrix IDs using the ExpressView feature.

Their gene expression analysis throughout the protocol matched that of a previously published profile for hESCs [24], which included the significant upregulation of mesodermal and chondrogenic markers (PDGFRB, SOX6, SOX9, ACAN, COL2A1 types A and B), with the more mature COL2A1 type B splice variant [42] being more highly expressed by the end of stage 3.

We selected 68 genes with putative immune - related functions, macroarrayed them and determined their expression profiles following bacterial or trypanosome challenge.

Saturday, Oct. 21, 11:20 - 11:40 a.m., South Hall B, South Building Featured Plenary Abstract: Massively parallel reporter assays combined with cell - type specific expression quantitative trait loci profiling identified a functional melanoma risk variant in HIV - 1 inhibitor gene, MX2 J. Choi, National Cancer Institute, et al

This method groups genes with similar expression profiles, an indication that they may function in similar pathways.

To better illustrate and map co-regulated genes with different activation and deactivation profiles, the levels of every transcript have been assigned to graphs of «Expression Waves» that follow a particular, predetermined, expression pattern (Expression Waves» that follow a particular, predetermined, expression pattern (expression pattern (Figure 5).

She emphasized several times the value of TCGA's GBM sequencing as a reference cancer genome, with high - quality mutations already profiled for mutations, gene expression, etc..

Specifically, we have generated clusters of transcripts that behave the same way under the entire spectrum of the sixty - seven experimental conditions; we have assembled genes in groups according to their time of expression during successive days of ES cell differentiation; we have included expression profiles of specific gene classes such as transcription regulatory factors and Expressed Sequence Tags; transcripts have been arranged in «Expression Waves» and juxtaposed to genes with opposite or complementary expression patterns; we have designed search engines to display the expression profile of any transcript during ES cell differentiation; gene expression data have been organized in animated graphs of KEGG signaling and metabolic pathways; and finally, we have incorporated advanced functional annotations for individual genes or gene clusters of interest and links to microarray and genomic expression during successive days of ES cell differentiation; we have included expression profiles of specific gene classes such as transcription regulatory factors and Expressed Sequence Tags; transcripts have been arranged in «Expression Waves» and juxtaposed to genes with opposite or complementary expression patterns; we have designed search engines to display the expression profile of any transcript during ES cell differentiation; gene expression data have been organized in animated graphs of KEGG signaling and metabolic pathways; and finally, we have incorporated advanced functional annotations for individual genes or gene clusters of interest and links to microarray and genomic expression profiles of specific gene classes such as transcription regulatory factors and Expressed Sequence Tags; transcripts have been arranged in «Expression Waves» and juxtaposed to genes with opposite or complementary expression patterns; we have designed search engines to display the expression profile of any transcript during ES cell differentiation; gene expression data have been organized in animated graphs of KEGG signaling and metabolic pathways; and finally, we have incorporated advanced functional annotations for individual genes or gene clusters of interest and links to microarray and genomic Expression Waves» and juxtaposed to genes with opposite or complementary expression patterns; we have designed search engines to display the expression profile of any transcript during ES cell differentiation; gene expression data have been organized in animated graphs of KEGG signaling and metabolic pathways; and finally, we have incorporated advanced functional annotations for individual genes or gene clusters of interest and links to microarray and genomic expression patterns; we have designed search engines to display the expression profile of any transcript during ES cell differentiation; gene expression data have been organized in animated graphs of KEGG signaling and metabolic pathways; and finally, we have incorporated advanced functional annotations for individual genes or gene clusters of interest and links to microarray and genomic expression profile of any transcript during ES cell differentiation; gene expression data have been organized in animated graphs of KEGG signaling and metabolic pathways; and finally, we have incorporated advanced functional annotations for individual genes or gene clusters of interest and links to microarray and genomic expression data have been organized in animated graphs of KEGG signaling and metabolic pathways; and finally, we have incorporated advanced functional annotations for individual genes or gene clusters of interest and links to microarray and genomic resources.

From a total of 23 samples, we used high - throughput cDNA sequencing (RNA - Seq) on the Illumina platform to profile gene expression between genders and tissues, with and without pairing influence.

The team compared the gene - expression profiles of samples from individuals with acute myeloid leukemia and acute lymphoblastic leukemia and identified 141 genes that were differentially expressed between the two groups.

Unbiased clustering analysis of P (+) neurons revealed four different classes, each with distinct cell surface receptor gene expression profiles.

On the publication of the article «Gene Expression Profiling in the Hibernating Primate, Cheirogaleus Medius», with the participation of José Luis Villanueva - Cañas (Josefa Gonzalez's group):

Sirtuin activators, notably resveratrol (which activates both SIRT1 and PGC - 1α, and improves the functioning of the mitochondria) 23 24 have been shown to inhibit gene expression profiles associated with muscle aging and age - related cardiac dysfunction in mice and may extend lifespan.25 26

Muscle biopsies from the study showed «a remarkable reversal of the expression profile of 179 genes associated with age and exercise training... Genes that were down - regulated with age were correspondingly up - regulated with exercise, while genes that were up - regulated with age, were down - regulated with exercise.&rgenes associated with age and exercise training... Genes that were down - regulated with age were correspondingly up - regulated with exercise, while genes that were up - regulated with age, were down - regulated with exercise.&rGenes that were down - regulated with age were correspondingly up - regulated with exercise, while genes that were up - regulated with age, were down - regulated with exercise.&rgenes that were up - regulated with age, were down - regulated with exercise.»

Based on the gene expression profile, we demonstrated the up - regulation of hormone sensitive lipase and enhancement of the lipolytic activity by the treatment of adipocytes with C3G or Cy.

In this study, we have shown the gene expression profile in human adipocytes treated with anthocyanins (cyanidin 3 - glucoside; C3G or cyanidin; Cy).

In this study, we have shown for the first time the gene expression profile in isolated rat adipocytes treated with anthocyanins (cyanidin 3 - glucoside; C3G or cyanidin; Cy).

The altered microbiota was associated with changes in colonic short - chain fatty acid (SCFA) concentrations, colonic - tissue gene expression (Gsta2 and Ela1), and host physiology (serum metabolite profiles and colonic goblet cell numbers).

In concurrent studies, the team will examine the gene expression profile of subsets of patients with different clinical outcomes to provide further insight into why certain dogs have more aggressive B - CLL and to identify possible drug targetable pathways to improve the treatment for this cancer.

Gene expression profiling in persons with multiple chemical sensitivity before and after a controlled n - butanol exposure session