Sentences with phrase «with gene silencing»

In the third generation, NT4.1, shows significantly less Basta resistant plants that predicted, consistent with gene silencing.

Today brings news that the first Huntington's Disease patients have been successfully dosed with gene silencing drugs targeting the HD gene.

Not exact matches

A version of this article appears in print on, on Page C3 of the New York edition with the headline: Merck Buys Maker of Gene - Silencing Drugs.

Silencing five genes whose expression negatively correlated with caspase activity significantly increased cancer cells» sensitivity to radiation (Genome Research, DOI: 10.1101 / gr.122044.111).

In Martin's view, the result strongly suggested that the patients had inherited the silenced gene from one of their parents, like the case with agouti mice.

This means that once the PIWI protein from the nucleus is loaded with the new piRNAs, it's able to target the right gene in the DNA and silence it more efficiently.»

The bits, called short interfering RNA, then associate with other enzymes to form a large complex that can silence certain genes and render the virus harmless.

The team created flies with a type of RNA that silenced certain genes.

In both groups, 30 per cent of flies died, with specific defects showing that the silenced genes controlled mainly early - stage development.

One of Beaudet's graduate students — Linyan Meng — was writing her dissertation on Angelman syndrome and was wrestling with this problem when a member of her dissertation committee, Dr. Thomas Cooper, professor of pathology & immunology at Baylor, said he was working with a Carlsbad, Calif. - based company called Isis Pharmaceuticals that had anti-sense oligonucleotides that could turn off the antisense transcript that silenced the paternal copy of the gene.

Unlike many genetic syndromes that are caused by a mutation in a gene, people with Prader Willi often have the right gene available — it's simply that it's been silenced.

It silences many other genes as well, but in those cases it usually acts with another protein.

The researchers treated 63 cancer cell lines (26 breast, 14 colorectal and 23 ovarian) with low - dose 5 - azacitidine (AZA), an FDA - approved drug for myelodysplastic syndrome, that reverses epigenetic changes by stripping off the methyl group that silences the gene.

Molecules of dsRNA are known to travel between body cells (any cell in the body except germ cells, which make egg or sperm cells) and can silence genes when their sequence matches up with the corresponding section of a cell's DNA.

«So, with this knowledge, the dream is that we could eventually use gene editing technology to edit the microRNA target sites in the host plants, preventing the microRNAs from binding and silencing these genes.

Silencing of one of the «cell death» genes may be the first step in finding treatments to help woman delay menopause or avoid the health problems — osteoporosis, heart disease — associated with the cessation of estrogen production.

So the group introduced mutations that silenced genes associated with autism spectrum disorders in mice, adding them in a way that restricted the effects to peripheral nerve cells, they report today in Cell.

The researchers at Bonn University and their colleagues from the Federal Institute of Drugs and Medical Devices (BfArM) and from the University of Regensburg worked with so - called transgenic and knockout mice in whom the gene for micro-RNA 155 was either increased or silenced.

Double - stranded RNA can silence the expression of a gene with a corresponding sequence, a process called RNA interference (RNAi).

Both mice and humans with a silenced FMR1 gene have malformed neurons: Spines on their dendrites are longer, thinner, and more numerous than normal, and they also transmit weaker electric signals.

«If cells with a certain gene silenced disappear, for example, it means that gene is essential for proliferation.»

Then they generated larvae with a suppressed glycosidase activity by silencing the respective larval glycosidase gene to study the effect of lyciumoside IV ingestion on the disabled larvae.

Day and colleagues propose a model where ecRNAs directly interact with the catalytic domain of DNA methyltransferases to block the ability of the methyltransferases to target and silence the overlapping gene for each specific ecRNA.

Silencing the BCL11A gene largely reversed those symptoms, with no apparent side effects, the group reports today online in Science.

To test their theory, the researchers investigated what would happen to fetal mouse brains if they interfered with Trnp1 expression using synthetic sequences of genetic material that silenced the gene, a technique called RNA interference.

Our and previous findings suggest that both HDACs and cytosine methylation contribute to HIV - 1 latency, in agreement with a growing body of evidence demonstrating cooperation between these two gene silencing mechanisms [55], [56].

Results with formulations of lipid nanoparticles as agents to deliver siRNAs in animals, including nonhuman primates, 28,29 have shown activity toward silencing of disease - modifying genes expressed in the liver.

LADs themselves are instead associated with gene - poor regions and gene silencing [43, 44].

What these groups are attempting basically is «gene silencing», with a twist - it's aimed directly at the DNA, rather than targeting a «message molecule» called RNA.

Since RNA - based gene silencing drugs have been successful so far, why bother with the greater challenge of targeting the DNA of the huntingtin gene itself, especially if it means dealing with virus particles and big, fragile drugs made of protein?

The Schizosaccharomyces pombe hst4 + gene is a SIR2 homologue with silencing and centromeric functions.

ALS can be caused by a mutation in a protein called SOD1, and the antisense drug effectively silences the gene that codes for this mutant protein — found in the cells of patients with inherited forms of ALS.

Ullu was recognized for her lab's work with the protozoan parasite Trypanosoma brucei, which causes African sleeping sickness, to uncover a novel mechanism of gene silencing known as RNA interference.

The histone proteins are decorated with different marks, which can affect the various activities of the modified DNA such as transcription, gene silencing, imprinting and replication.

Epigenetics began as a correlative field in which covalent modifications to DNA or histones, the proteins that help package DNA, were associated with gene expression or silencing.

In the latest version of the hide - and - seek game between pathogens and the hosts they infect, researchers have found that a virus appears to cloak itself with a recently discovered gene silencing...

They accumulate at sites of attachment and come in contact with parts of certain silenced genes.

Western blot analysis showed almost complete silencing of AKT1 gene product that was associated with decreased levels of p - mTOR (C).

We also show that inhibition of mTOR with rapamycin, as well as silencing mTOR gene product expression using mTOR - specific small interfering RNA, decreased phosphorylation of mTOR signaling proteins and induced cell cycle arrest and apoptosis in ALK + ALCL cells.

ONE - PARENT SAMPLE SET PARENTAL GUIDANCE RULES: Maternally methylated (red dots) and paternally methylated (blue dots) regions across the human autosomal chromosomes, based on studies of uniparental disomy samplesGENOME RES, 24:554 - 69, 2014 RESEARCHERS Kazuhiko Nakabayashi, Division Chief, Department of Maternal - Fetal Biology, National Research Institute for Child Health and Development, Tokyo, Japan; David Monk, Principal Investigator, Epigenetics and Cancer Biology Program, Bellvitge Institute for Biomedical Research ORGANISM Human METHODS Bisulfite - seq; bisulfite - chip Methylation is typically associated with the silencing of the nonexpressed allele, making it a convenient marker for imprinted genes, though it's possible for patterns of differential methylation to exist in tissues where both alleles are expressed.

What's more, it can be given with a simple injection into the blood, offering great potential for research in gene silencing research and beyond.

They discovered that CRISPRi was much more efficient than CRISPR - Cas9: in more than 95 % of the cells created using CRISPRi, the target gene was silenced, compared with only 60 - 70 % of cells grown from CRISPR - Cas9.

Features a clinical trials roundup, an exclusive interview with Prof Sarah Tabrizi about the first trial of a huntingtin lowering «gene silencing» drug, and a surprise for EHDN president Prof Bernhard Landwehrmeyer.

«With the new stem cell model, however, we hope eventually to put together a much more detailed picture of this process of repeat expansion and gene silencing, and that should put us in a good position to devise optimal therapies,» Gottesfeld said.

Briefly, NHLF cells were grown in 96 - well CoStar tissue culture plates (4,000 cells per well) and either subjected to siRNA gene silencing or directly treated with compounds for 51hours in 100 µL complete medium per well.

«With the new stem cell model, we hope eventually to put together a much more detailed picture of this process of repeat expansion and gene silencing, and that should put us in a good position to devise optimal therapies,» says Professor Joel Gottesfeld.

«Now that we have the gene, we can actually use that gene sequence to temporarily silence the gene and make rye and other chromosomes pair with wheat and transfer genes by a natural method into wheat without calling it GMO,» Gill said.

Genes associated with melanoma are overexpressed or silenced with shRNA constructs in lentiviral vectors and the lab increasingly uses cDNA and sh (short hairpin) RNA libraries for our experiments.

Cenix BioScience is a contract research organization focused on the discovery and pre-clinical development of novel therapeutics, specializing in advanced applications of RNA interference (RNAi) gene silencing combined with high content phenotyping in cultured cells in vitro and in animals in vivo.

Comparing this approach to the classic CRISPR / Cas9 system designed to cleave the DNA, the Gladstone team found that CRISPRi is actually more effective, silencing the target gene in more than 95 percent of cells, compared with the 60 percent to 70 percent efficiency of CRISPR / Cas9.