Sentences with phrase «with gene transfer»

In the 1980s, Dr. Kaufman's experience with gene transfer and engineering led him to become a founding scientist at Genetics Institute Inc., where he engineered mammalian cells for high - level expression of therapeutic proteins, such as clotting factors that are now used to treat individuals with hemophilia.

CTL119 manufacturing begins with a patient's own T cells, some of which are removed and then reprogrammed in Penn's Clinical Cell and Vaccine Production Facility with a gene transfer technique designed to teach the T cells to target and kill tumor cells.

Not exact matches

OAR proponents claim that when the altered donor - cell nucleus with its activated nanog gene is transferred to the enucleated oocyte (egg cell), the presence of nanog will immediately convert the enucleated egg cell to a pluripotent cell, without ever forming a zygote.

With respect to antibiotic resistance, this novelty can be induced and we can trace the novelty to particular mutations arising absent gene transfer; i.e. the genes which give rise the resistance were not present prior to the mutation, they are new sequences and not simply the activation of dormant genes.

Once specific genes associated with beneficial traits are identified, they can be more efficiently transferred into new rice varieties using other breeding methods.

«It is an intriguing question as to how this Enterococcus strain acquired a botulinum neurotoxin gene cluster, what benefit it brings to this bacterium, and further work is required to explore the implications of our important finding with regard to the possibility of its transfer between bacteria.

«When you contaminate a site with resistance genes, those genes can be transferred into environmental organisms and actually increase the concentration of contamination.»

Issued last March to researchers at a little - known cotton seed company called Delta & Pine Land (D&PL) and the U.S. Department of Agriculture, the patent covers a technique for transferring three genes along with their genetic on switches into the seeds of genetically improved plants.

Three options are on the table: tweak cereals so that they form symbiotic partnerships with rhizobia as legumes do; colonise cereal roots with other types of nitrogen - fixing bacteria; or transfer the bacterial genes that make fertiliser directly into the crop plants.

For the dozens of labs at work on the problem, coming up with useful genes, known as transgenes, is not the hard part — it's how to transfer enough copies of the transgenes into free - ranging populations.

Bacteria lacking them to start with can simply borrow them (via horizontal transfer of genes) from those that are better equipped.

But by investigating the DNA sequence around the resistance genes, the team figured out how the resistance genes transfer occurred through a new mechanism named «carry back,» where the pathogen basically has a primitive form of «sex» with the Actinobacterium and takes up its resistance genes after it dies.

Even distantly related bacteria can swap genes with one another using a variety of techniques, from direct cell - to - cell transfer, called conjugation, to transformation, in which a bacterium releases snippets of DNA that other bacteria pick up and use.

«Dangerous bacteria found after sewer spills: Bacteria equipped with genes that can transfer antibiotic resistance adds to a sewage spill's public health threat.»

It is perhaps thus likely that that these damaged genes coding for psychiatric diseases are overrepresented in women with fertility problems, and, if transferred to their offspring, this may at least partly explain the increased risk of psychiatric diseases.»

Imagine researchers» excitement, then, when Kyoto University's Shinya Yamanaka reported last year that his group had created embryolike cells directly, without transferring chromosomes, simply by injecting mouse fibroblast cells with four genes that are active in embryonic cells but not in adult ones.

This is because the fungal highway enables pollutant - degrading bacteria to reach their food faster and, with the help of gene transfer, they may even be upgraded along the way.

Wick continues: «Our study shows that fungal hyphae not only provide soil bacteria with an excellent infrastructure, but also a potential hot spot for bacterial horizontal gene transfer.

So in an attempt to cure mice of hepatitis B, Kay's team flooded their livers with double - stranded RNA from a gene transfer vector.

Bacteria share DNA with other organisms with which they come into contact, so an antibiotic - resistant gene can transfer from one type of bacterium to another, and a bacterial cell can become resistant to numerous antibiotics at once.

Beginning with the Nazi Doctors» Trial at the 1946 Nuremberg Trial (1), coverage includes publication of Henry Beecher's «Ethics and Clinical Research» (2), The New York Times exposure of the public health service syphilis study in Macon County, Alabama (the infamous «Tuskeegee case»)(3), the University of Pennsylvania / Gelsinger gene transfer case, and The Washington Post series on international clinical drug testing abuses (4).

The new method circumvents the problem of not being able to culture many of these bacteria in the lab by transferring genes from these bacteria into another bacterial species that is easier to work with.

Moreover, Boothby showed that yeast and bacteria artificially equipped with the genes could also survive dehydration, suggesting that they could potentially be transferred into crops to help them survive drought.

«The chance of the gene being lost over and over again in the other lineages seems vanishingly small compared with a single transfer from a metazoan to a bacteria.»

This gene, along with a number of other genes encoding proteins involved in nickel homeostasis, has been acquired a long time ago by the bacterium via horizontal gene transfer.

Given that tens of thousands of parasitic wasp species, each associated with a unique bracovirus, parasitize virtually all lepidopteran species, it is likely that the described phenomenon is general and that different gene transfers occur regularly in nature.

Or they could acquire the ability while exchanging bits of DNA with other E. coli in a process called horizontal gene transfer.

In the new research, Prins and Liau used a technique called adoptive cell transfer, which involves extracting and growing immune cells outside of the body, then reprogramming them with a gene known as New York Esophageal Squamous Cell Carcinoma, or NY - ESO - 1.

The team also confirmed differences in mating strategies, notably that the pathogenic fungus can mate with itself, which influences gene transfer between generations and genome repair mechanisms, whereas the Quorn ™ fungus would need to find a mate.

Bengt Nordéns contribution to form a strong research school in Gothenburg has been successful: as many as 12 out of his about 50 former PhD students and postdocs have become professors, abroad or at other Swedish universities, and three have returned to contribute a forceful environment with their own profiles within the Department: Prof Bo Albinsson (femtosecond spectroscopy and fundamentals of electron transfer), Prof Per Lincoln (new transition - metal - based DNA ligands and statistical mechanics for gene targeting), Prof Björn Åkerman (fundamentals and applications of DNA physical chemistry).

However, like most gene transfer approaches a major concern with ZFN technology is the potential for oncogenesis due to off - target effects.

To avoid any duplication within the IMPC, a gene already reserved by another center (i.e. already produced, or with production underway, or planned to be produced) will not be selected but the production state will be transferred to the requestor.

Using a novel technique to genetically modify T cells for adoptive transfer, Carl June, Michael Kalos, David Porter, Bruce Levine, and colleagues at the University of Pennsylvania School of Medicine achieve clinical responses in patients with chronic lymphocytic leukemia, including two complete, durable (one year) clinical responses, accompanied by in vivo expansion and long - term functional persistence of gene - modified cells.

These cells are easily accessible (therefore, they are the first ones used in the clinic), seemingly well suited for gene transfer, and used in practice with new genes to correct two types of severe combined immunodeficiency (which are briefly described in the book).

Mammalian Y chromosome genes with important functions are transferred to autosomal chromosomes more often than previously thought, a study shows.

«One way of treating patients with limb - girdle muscular dystrophy might be to use a gene transfer approach to treat the muscular dystrophy, and a pharmacological approach to treat the smooth muscle dysfunction,» he said.

Concerns about immunological consequences of rAAV - mediated gene transfer were substantiated by the outcome of a clinical trial in which human hemophilia B patients were infused into the liver with rAAV -2-F.

Manno, Catherine et al. «AAV - Mediated Factor IX Gene Transfer to Skeletal Muscle in Patients with Severe Hemophilia B.» Blood.

Specific activities include optimizing the conduct and oversight of gene transfer research, updating and interpreting biosafety policies under the NIH Guidelines Involving Research with Recombinant and Synthetic Nucleic Acids, providing critical input on governmental policies regarding dual use research, the NIH Stem Cell Registry.

Draft genome annotation was transferred from P. vivax P01 using RATT21 (version 1), and supplemented with the output of the Augustus22 gene finder, trained on P. vivax P01 as described in23.

Years after receiving a single dose of gene therapy, patients on the hemophilia B trial continued to produce their own clotting factor from the normal transferred gene with minimal side effects.

He was a Helen Hay Whitney fellow with Nobel Laureate Dr. Phillip Sharp at the Center for Cancer Research at the Massachusetts Institute of Technology (M.I.T.), where he developed gene transfer technologies based on gene amplification and expression in mammalian cells.

«Now that we have the gene, we can actually use that gene sequence to temporarily silence the gene and make rye and other chromosomes pair with wheat and transfer genes by a natural method into wheat without calling it GMO,» Gill said.

If ARM genes were to transfer to pathogenic bacteria inside the gut or mouse, they might create super diseases, untreatable with one or more types of antibiotics.

AAV gene therapy vectors are emerging as the gene transfer vehicle with high potential for use in the CNS as they transduce post mitotic cells that mediate the sustained, long term gene expression required in chronic progressive diseases.

Fetal NSCs were infected with both Lenti - Oct4 and Lenti - Nanog at a titer of 0.5 × 1010 gene transfer units / ml overnight, followed by a 2 - day recovery period before being plated on mitotically inactive MEFs.

A breakthrough came in 1996 with the introduction of Bt cotton, a genetically engineered crop containing a gene transferred from the bacterium, Bacillus thuringensis, endowing the plants with a protein that kills some, but not all insects.

Integrons capture and disseminate gene - cassettes; short circular pieces of DNA that may contain resistance genes [35], and integrons associated with class 1 integrases are known to transfer many forms of resistance genes [36].

In fact, during the long course of evolution, this gradual transfer of genetic information into the nucleus has already occurred with the majority of mitochondrial genome, leaving behind a mere 13 protein coding genes within the mitochondria.

Elements associated with horizontal gene transfer identified in the metagenomes.