Sentences with phrase «with human cancer cell lines»

Not exact matches

«This model was trained on genetic data from human tumors in The Cancer Genome Atlas and was able to predict response to certain inhibitors that affect cancers with overactive Ras signaling in an encyclopedia of cancer cell lines,» GreeneCancer Genome Atlas and was able to predict response to certain inhibitors that affect cancers with overactive Ras signaling in an encyclopedia of cancer cell lines,» Greenecancer cell lines,» Greene said.

Professor Ali Tavassoli, who led the study with colleague Dr. Ishna Mistry, explains: «In an effort to better understand the role of HIF - 1 in cancer, and to demonstrate the potential for inhibiting this protein in cancer therapy, we engineered a human cell line with an additional genetic circuit that produces the HIF - 1 inhibiting molecule when placed in a hypoxic environment.

Using both fruit fly and human lung cancer cell lines, researchers targeted two of the most common genetic mutations associated with NSCLC — Ras and PTEN (P13K).

Tests with kidney cancer and two human uterine sarcoma cell lines, one with multidrug resistance, showed that 10 of these new compounds were impressively potent against all three cell lines, the researchers reported.

In the lab, the scientific team used an approach that combined functional RNAi analysis with gene expression analysis in breast cancer - derived cell lines and in human breast cancers replicated in mice.

In a paper published online May 17, 2016 in the journal Nature Communications, de Jong and co-first author Koji Taniguchi, MD, PhD, and colleagues, report that treating both ERK1 / 2 and the compensatory pathway, ERK5, concurrently with a combination of drug inhibitors halted colorectal cancer growth more effectively in both mouse models and human colorectal cancer cell lines.

Dr. Bauman and her colleagues treated human head and neck cancer cells in the laboratory with varying doses of sulforaphane and a control, and compared them to normal, healthy cells that line the throat and mouth.

Recent studies with human breast cancer cell lines have also shown that Shp2 mediates survival signals in cancer cells.

For the time course study, cells were treated with 20 μM of EGCG for 12, 24, 48, 72, or 144 h. Human colon cancer cell line HT - 29 and prostate cancer cell line PC3 were obtained from American Type Culture Collection (Manassas, VA), and were grown in McCoy's 5A and RPMI 1640 containing 10 % fetal bovine serum, respectively.

Sphere - forming cell subpopulations with cancer stem cell properties in human hepatoma cell lines.

The membrane is coated with proteins that support 3 - D cell growth and it's seeded with human intestinal epithelial cells (from a cell line originally isolated from a colon cancer).

They fused mouse B cells — antibody - producing cells of the immune system — with human myeloma (also known as B cell cancer) cell lines in a new technique called hybridoma technology.

Pim - 3, a Proto - Oncogene with Serine / Threonine Kinase Activity, Is Aberrantly Expressed in Human Pancreatic Cancer and Phosphorylates Bad to Block Bad - Mediated Apoptosis in Human Pancreatic Cancer Cell Lines

I wanted to inactivate the gene BRAF (a kinase implicated in several human cancers) in A549 cells (a human lung cancer cell line), armed only with viruses obtained through Addgene's viral service and the methods sections of scientific articles (gasp).

(a kinase implicated in several human cancers) in A549 cells (a human lung cancer cell line), armed only with viruses obtained through Addgene's viral service and the methods sections of scientific articles (gasp).

The human breast cancer cell line SKBR3 was purchased from the Duke University Cell Culture Facility and was maintained in McCoy's 5A medium (Life Technologies) supplemented with 10 % FBS (Life Technologies) and antibiotcell line SKBR3 was purchased from the Duke University Cell Culture Facility and was maintained in McCoy's 5A medium (Life Technologies) supplemented with 10 % FBS (Life Technologies) and antibiotCell Culture Facility and was maintained in McCoy's 5A medium (Life Technologies) supplemented with 10 % FBS (Life Technologies) and antibiotics.

In a series of lab experiments with cell lines, human xenograft tumors in mice and primary human prostate cancer samples, the researchers demonstrated that miR - 34a inhibits prostate cancer stem cells by suppressing CD44.

Cultures of the human triple negative breast cancer cell line, HCC1806, were maintained in RPMI 1640 medium supplemented with 10 % FBS.

Important features of XMRV biology include (1) tropism for a variety of cell lines, including prostate cancer DU145 and LNCaP cells [27], [43], [48], and human neural cell types [57], (2) adaptations that promote growth in prostate epithelium and human - derived prostate cancer cell lines including an androgen response element in the promoter region [58] and downregulation of APOBEC3G [59], and (3) cellular effects with potential oncogenic properties including increased tumor aggressiveness mediated by downregulation of p27 [60] and differential regulation of several microRNAs [61].

In collaboration with Nanyang Technological University, experiments were also conducted to co-deliver paclitaxel and small interfering RNA (siRNA) targeting a protein that prevents cell death (Bcl - 2) to MDA - MB - 231 human breast cancer cell line.

To confirm specificity, supernatant from transfected or transduced HB1.F3 cells was incubated with MCF7, MCF7 / HER2, or BT474, a human breast cancer cell line that endogenously overexpresses HER2.

Serenoa repens (Permixon) inhibits the 5alpha - reductase activity of human prostate cancer cell lines without interfering with PSA expression..

Bottom line While research has shown some benefits with regards to ER + breast cancer cell death and prevention of metastases within mice and cellular models, it is recommended that human intake should be through diet only, not supplementation.