Sentences with phrase «with human cell cultures»

We thank K. Saul, D. Harlow and K. Ellison for conducting histology and behavioral testing (Department of Neurosurgery, UC Denver) and Michelle Lacagnina (Department for Biomedical Genetics, University of Rochester, NY) and Laurie Baxter (Surgical Pathology, University of Rochester, NY) for assistance with human cell cultures, and Brendan Carlin for assistance with RT - PCR analysis (Department for Biomedical Genetics, University of Rochester, NY).

Not exact matches

The researchers experimented with inducing oxidative stress in a human cell line culture with and without VCOP (virgin coconut oil polyphenols) to observe how VCOP positively promoted catalase, a very important enzyme in protecting the cell from oxidative damage, and glutathione (GSH), a self - recycling antioxidant produced by the liver.

Visually, she is filming and analyzing time - lapse images of human embryos in the incubator and has been able to correlate various parameters of how cells divide with the probability that the embryos will make it to a full blastocyst stage by day 5 - 6 of culture.

But when the researchers added higher concentrations of BX795 to cultured human corneal cells infected with HSV - 1, the infection was quickly cleared.

Shukla and colleagues discovered that a small drug molecule called BX795, which is sold to labs for use in experiments, helped clear HSV - 1 infection in cultured human corneal cells, in donated human corneas, and in the corneas of mice infected with HSV - 1.

The Wyss team believes the ability of the human gut - on - a-chip to culture the microbiome with human gut cells also holds promise for the field of precision medicine, where a patient's own cells and gut microbiota could one day be cultured inside a gut - on - a-chip for testing different therapies and identifying an individualized treatment strategy.

With our human gut - on - a-chip, we can not only culture the normal gut microbiome for extended times, but we can also analyze contributions of pathogens, immune cells, and vascular and lymphatic endothelium, as well as model specific diseases to understand complex pathophysiological responses of the intestinal tract.»

Confocal microscopy of human neural stem cell culture infected with Zika virus (red).

Follow - up tests in human cell cultures confirmed that MIR168a interferes with production of a cholesterol - clearing protein.

In studies with human fibroblasts that make up connective tissue, Boger's team tested whether NOD1 activity could affect CMV replication in cultures of cells grown in the lab.

Greber and his team infected human cells in culture with the chemically labeled viruses, and observed the behavior of the viral DNA during entry into cells.

While there, he worked with cell cultures derived from human fetal tissue.

They found that indeed, they do, and that stimulating these cells led them to kill cells infected with HIV - 1 derived from latently infected cells, both in culture and in mice engineered to have a human immune system.

The scientists transferred the clumps to cultures where other human ES cells, marked with green fluorescent protein, were already growing.

Hang and coworkers exposed the human cells by first extracting the compounds from the paper with a culture medium then using the medium to culture the human cells for 24 hours.

«We culture typical skin cell of the epidermis, such as human keratinocytes, in our dishes to form an artificial epidermis with all of its natural layers,» explained Sibylle Thude, the biologist who led the investigation into the accreditation.

Finally, they demonstrated that zebrafish OPCs differentiate into mature oligodendrocytes when cultured together with human motor neurons, differentiated from induced pluripotent stem cells.

Prior research with cultured tissue had shown that a mix of chemicals could change bone marrow stem cells from mice to those resembling brain cells, but when a team led by neurologist Lorraine Iacovitti of Thomas Jefferson University in Philadelphia tried the same brew on human cells, the number altered was modest.

Drugs that activate this novel stress response pathway, which they call the mitochondrial - to - cytosolic stress response, protected both nematodes and cultured human cells with Huntington's disease from protein - folding damage.

The researchers also cultured human skin cells and blood cells with the two compounds to test their toxicity.

But while researchers have previously been able to infect cultures of human hepatocytes with HBV, the cells» limited lifespan has made it difficult to study the virus, says Bhatia, who is also a Howard Hughes Medical Institute investigator and a member of MIT's Koch Institute for Integrative Cancer Research and Institute for Medical Engineering and Science.

Then, by placing surface proteins of each HCV virus onto these pseudoviruses, the researchers were able to efficiently infect human cells with the HCV strains in tissue culture.

The investigators studied application of various doses of a common PBDE flame retardant on human adrenal cells in culture dishes and compared the effects with those of only the vehicle, the inactive substance used to deliver the chemical.

Zhao says his group plans to further optimize the culture conditions and eventually attempt to repeat the feat with human cells.

Séralini's report in BioMed Research International describes how pesticides kill cultured human cells, with the hair - raising conclusion that pesticides may be vastly more toxic than assumed by regulatory authorities.

Before toying with worms, the S.U.N.Y. Buffalo team tried out their technique on cultures of human embryonic kidney cells and neurons from a rat hippocampus, a part of the brain integral to memory.

The new study, led by Walter Mothes, a Yale microbial pathogens expert, involved creating one culture that mixed healthy rat cells with cells infected by the murine leukemia virus, a cancerous pathogen in rats and monkeys that is not known to affect humans.

By culturing human stem cells in this manner and treating them with specific signaling molecules, the investigators were able to guide cells through key processes involved in the development of the human inner ear.

Although a clause in the law that funds NIH prevents the agency from funding research that would harm or destroy an embryo, a lawyer at the Department of Health and Human Services ruled in 1999 that because stem cells — which can grow ad infinitum in culture — are not themselves embryos, the NIH could fund work with cells that were derived by privately funded researchers or researchers overseas.

When they put human macrophages in the lower compartment filled with cell culture medium, the cells rose toward the membrane.

Expanding from their previous studies with mice, the researchers first established that under specific conditions, culturing human embryonic stem cells with fibroblast growth factor 2 (FGF2) leads to neural differentiation particular to the midbrain / hindbrain region — the location of the cerebellum — within three weeks, and the expression of markers for the cerebellar plate neuroepithelium — the part of the developing nervous system specific for the cerebellum — within five.

For the time course study, cells were treated with 20 μM of EGCG for 12, 24, 48, 72, or 144 h. Human colon cancer cell line HT - 29 and prostate cancer cell line PC3 were obtained from American Type Culture Collection (Manassas, VA), and were grown in McCoy's 5A and RPMI 1640 containing 10 % fetal bovine serum, respectively.

Surgeons report success in first human bone growth procedure using fat stem cells — with no culturing necessary

Similar to the results obtained with cultured human cells, the transgenic mice were resistant to avian influenza viruses but susceptible to flu viruses of human origin.

In keeping with this, recent studies showed that flavanols, a subclass of flavonoids that is richly represented in natural cocoa beans, increase NO production by cultured human vascular endothelial cells (16) and improve endothelium - dependent vasorelaxation (NO - dependent) in finger (2) and brachial (3) arteries of healthy humans.

In cultured human cells and in rabbits with implanted stents, Finn and colleagues showed that metformin augmented the effect of mTOR inhibitors on regrowth of the blood vessel lining.

Human iPS cell - derived hepatocytes differentiated with our robust differentiation protocol and cultured using our novel maintenance medium provide an inexhaustible, consistent supply of functional hepatocytes that can be used to advance the understanding of diseases related to dysfunction in liver metabolism, including NAFLD / NASH, type 2 diabetes, and metabolic syndrome.

Once primary cultures were established, the cells were immortalized with human papilloma virus E6 and E7 oncogenes using the disabled recombinant retroviral vector pLXSN16E6 / E7 (26).

Human iPS cell - derived hepatocytes differentiated with our robust differentiation protocol and cultured using a novel maintenance medium provide an inexhaustible, consistent supply of functional hepatocytes that can be used to advance the understanding of diseases related to dysfunction in liver metabolism, including NAFLD / NASH, type 2 diabetes, and metabolic syndrome.

This enables robust stem cell culture methods that in practical solves all current technical challenges with culturing human ES and iPS cells.

7/16/2008 Improved Culture System for Hepatitis C Virus Infection A University of California, San Diego School of Medicine researcher has developed the first tissue culture of normal, human liver cells that can model infection with the Hepatitis C virus (HCV) and provide a realistic environment to evaluate possible... Culture System for Hepatitis C Virus Infection A University of California, San Diego School of Medicine researcher has developed the first tissue culture of normal, human liver cells that can model infection with the Hepatitis C virus (HCV) and provide a realistic environment to evaluate possible... culture of normal, human liver cells that can model infection with the Hepatitis C virus (HCV) and provide a realistic environment to evaluate possible... More...

Five years ago, the group reported that when they infected human tonsil cells with HIV in culture, 95 percent of the CD4 + T cells that died did so before the virus had a chance to reproduce within them.

Human satellite cell cultures display numerous features of mature skeletal muscles (1) and have been used for studies of muscle metabolism in cultures established from patients with type 2 diabetes and healthy control subjects (1 — 5,8,9,21,22).

The primary epithelial cells are expanded as 3D organoids, dissociated, and cultured on a porous membrane within a microfluidic device with human intestinal microvascular endothelium cultured in a parallel microchannel under flow and cyclic deformation.

Sorted human ILC3s (Lin - CD127 +) were cultured at 2,000 — 5,000 cells per well of a 96 well plate with irradiated (30 Gy) OP9 - DL1 feeders [70].

We combine it with the use of human tissue culture cells in which we validate some of the results obtained in egg extract.

The human breast cancer cell line SKBR3 was purchased from the Duke University Cell Culture Facility and was maintained in McCoy's 5A medium (Life Technologies) supplemented with 10 % FBS (Life Technologies) and antibiotcell line SKBR3 was purchased from the Duke University Cell Culture Facility and was maintained in McCoy's 5A medium (Life Technologies) supplemented with 10 % FBS (Life Technologies) and antibiotCell Culture Facility and was maintained in McCoy's 5A medium (Life Technologies) supplemented with 10 % FBS (Life Technologies) and antibiotics.

Assessment of hASC - CM composition found high expression of various human growth factors (IL ‐ 6, 8, 12, eotaxin, IP10, MCP ‐ 1, VEGF, and TIMP ‐ 1) in the supernatant following the co-culture of hASCs with islet cells, while IP10, eotaxin, VEGF, and TIMP ‐ 1 became increased with time during islet co ‐ culture, suggesting the presence of paracrine cross ‐ talk between islets and hASCs.

It is also worthy to mention that human ES cells and iPS cells could be maintained on the HFF cells with the conventional human ES culture medium (Li et al, unpublished data).

Here, we examine wild - type huntingtin's localization in cultured cells by expressing the full - length human protein tagged with enhanced green fluorescent protein (EGFP) within its unspliced genomic context.