During the last several years, research efforts
with human cell lines revealed that the genomic loci encoding tRNAs give rise to shorter tRNA fragments («tRFs»).
IHC - P mouse tumor tissue (from lung)
with human cell line injected, some muscle tissue attached as well sees high background for human cellswith priamry Ab as well as isotype ctrl, but also for muscle (does not contain any EGF) Ab: 1 ug /...
Not exact matches
The researchers experimented
with inducing oxidative stress in a
human cell line culture
with and without VCOP (virgin coconut oil polyphenols) to observe how VCOP positively promoted catalase, a very important enzyme in protecting the
cell from oxidative damage, and glutathione (GSH), a self - recycling antioxidant produced by the liver.
Cholesterol is carried in the blood and is infused into the
cells lining the blood vessels, says Prof. Seneviratne dealing
with the chemistry of the
human body.
Before we established in - house
human taste
cell (HTC) technologies, it was not possible to use
human tongue - derived
cells as a model, due to the lack of homogenous, proliferating
cell lines with defined properties, which is a prerequisite to establish comprehensive research and screening programs.
To create the effect of tobacco smoke on
cells, Vaz, Baylin and their colleagues began their studies
with human bronchial
cells, which
line the airways of the lungs, and grew them in a laboratory.
Introducing new drugs to plastic microchips
lined with human cells should let researchers watch what happens without ever involving a living creature.
- A Game of Hypothesizing, Dean Interpreting Data - Quantitative Analysis of Mixtures, Schwartz Defining Operationally - Biotic Community, Menhusen
Cells and Magnification, Menhusen Reading Activity - Formulating Hypothesis, Hebeisen Reading Exercise - Oersted's Experiment, Schwartz Formulating Hypotheses - Levers, Schwartz North by Compass, North by Shadow, Dennis Controlling Variables -
Human Heart Rate, Phillips Effect of Practice on Memorization, Forgetting and Relearning, Mayor Learning About
Line Graphs I, II and III, Dean Controlling Variables - Chemical Reactions, Livermore Chances Are, Hebeisen Interpreting Data - Animal Behavior, Menhusen Working
with Solutions, Dean Defining Operationally - Inertia and Mass Stamp Out Trash, Hebeisen Interpreting Data Module - A Limited Earth: An Unlimited Population?
«This model was trained on genetic data from
human tumors in The Cancer Genome Atlas and was able to predict response to certain inhibitors that affect cancers
with overactive Ras signaling in an encyclopedia of cancer
cell lines,» Greene said.
The work, funded by the US National
Human Genome Research Institute, aims to create human cell lines with subtly different genomes in order to test ideas about which mutations cause disease and
Human Genome Research Institute, aims to create
human cell lines with subtly different genomes in order to test ideas about which mutations cause disease and
human cell lines with subtly different genomes in order to test ideas about which mutations cause disease and how.
Although the researchers emphasized that laboratory results involving
cell lines and mice do not necessarily translate to
human treatment, they say their findings show that new mTOR inhibitors combined
with chemotherapy could become a new treatment strategy for T - ALL.
Human placental
cell line Jeg - 3 can be readily infected by a circulating strain of Zika virus (left panel) and completely protected by treatment
with nanchangmycin (right panel).
Professor Ali Tavassoli, who led the study
with colleague Dr. Ishna Mistry, explains: «In an effort to better understand the role of HIF - 1 in cancer, and to demonstrate the potential for inhibiting this protein in cancer therapy, we engineered a
human cell line with an additional genetic circuit that produces the HIF - 1 inhibiting molecule when placed in a hypoxic environment.
Using both fruit fly and
human lung cancer
cell lines, researchers targeted two of the most common genetic mutations associated
with NSCLC — Ras and PTEN (P13K).
Cell lines from
human tumours did exist, but were considered unsafe: what if cancerous
cells were transferred along
with the vaccine?
In May 2005, Hwang and his colleagues reported that it had produced 11 new
human embryonic stem (ES)
cell lines that carried the genetic signature of patients
with diabetes, spinal cord injury, or a genetic blood disorder (Science, 20 May, p. 1096).
Tests
with kidney cancer and two
human uterine sarcoma
cell lines, one
with multidrug resistance, showed that 10 of these new compounds were impressively potent against all three
cell lines, the researchers reported.
While he has been working
with federally - approved
human ES
lines, he notes that those
lines could be considered illegal until they get vetted by the stem
cell working group NIH plans to create.
The final guidelines on research
with human embryonic stem
cells issued on Monday by the National Institutes of Health set out criteria for determining which ES
cell lines can be used in federally funded experiments and give NIH discretion to approve old
lines that don't meet stringent modern ethical requirements.
The bill was put forth to loosen the restrictions Bush placed on
human embryonic stem
cell research on August 9, 2001, when he banned federal funding for work
with any stem
cell line created after that date.
In the chamber, tubes about the thickness of a
human hair were
lined with endothelial
cells as in natural blood vessels.
After exploiting a technology that allowed them to activate each of nearly 16,000 genes individually in
human melanoma
cell lines containing mutant BRAF, the authors then treated the panel of
cells with the drugs and monitored which
cells showed altered drug sensitivity.
In a statement put on the Web this morning, NIH reported that all 64
lines «show characteristics of stem
cell morphology» and have undergone several population doublings, and most of them have demonstrated all the protein markers «known to be associated
with human embryonic stem
cells.»
In the lab, the scientific team used an approach that combined functional RNAi analysis
with gene expression analysis in breast cancer - derived
cell lines and in
human breast cancers replicated in mice.
Earlier this year, researchers in Germany published a scientific paper that described the first sequence of the full HeLa genome, comparing the DNA of HeLa
cell lines with that of
cells from healthy
human tissues.
«
With our sequencing and transcriptional data, we can figure out which of these
cell lines most closely match the
human tumors.
In a paper published online May 17, 2016 in the journal Nature Communications, de Jong and co-first author Koji Taniguchi, MD, PhD, and colleagues, report that treating both ERK1 / 2 and the compensatory pathway, ERK5, concurrently
with a combination of drug inhibitors halted colorectal cancer growth more effectively in both mouse models and
human colorectal cancer
cell lines.
His team have begun recolonising the primate scaffolds
with human cells that
line blood vessels, the first step towards
human - scale biolimb development, and have started experiments using
human myoblasts in rats instead of the mice ones.
To study this barrier and determine why a lack of blood flow causes it to leak, the researchers built a blood - vessel - on - a-chip model consisting of a channel
lined with a layer of
human endothelial
cells surrounded by extracellular matrix within a microfluidic device, which allowed them to easily simulate and control the flow of blood through a vessel and evaluate the
cells» responses.
«We set out to test this in
cell lines derived from mice and
humans, and came up
with a result we definitely did not expect — which then led us to dig deeper for answers.»
Dr. Bauman and her colleagues treated
human head and neck cancer
cells in the laboratory
with varying doses of sulforaphane and a control, and compared them to normal, healthy
cells that
line the throat and mouth.
They tried hundreds of different recipes; eventually they discovered that if they mixed liver precursor
cells (derived from iPS
cells)
with two other types of standard
human cell lines known to be important for embryonic liver development, then the
cells would spontaneously form a 4 to 5 - millimeter 3D structure called a liver bud.
«If there are 60
cell lines, that's news to me — and good news,» says
cell biologist Douglas Melton of Harvard University, who has been working
with both animal and
human ES
cells.
Attempting to find middle ground in the contentious debate over
human embryonic stem
cells, President George W. Bush announced 9 August that the National Institutes of Health (NIH) will be allowed to fund work
with embryonic stem (ES)
cell lines — but only those
lines that have already been derived.
The
human airway is
lined with two kinds of epithelial
cells — those that possess hairlike fringes called cilia and those that don't.
Despite the ability of R3A to utilize both CCR5 and CXCR4 to infect
cell lines, in
human CD4 + T
cells stimulated
with anti-CD3 / anti-CD28 coated magnetic beads, CCR5 is downregulated causing transient resistance to R5 HIV [47].
Recent studies
with human breast cancer
cell lines have also shown that Shp2 mediates survival signals in cancer
cells.
For the time course study,
cells were treated
with 20 μM of EGCG for 12, 24, 48, 72, or 144 h.
Human colon cancer
cell line HT - 29 and prostate cancer
cell line PC3 were obtained from American Type Culture Collection (Manassas, VA), and were grown in McCoy's 5A and RPMI 1640 containing 10 % fetal bovine serum, respectively.
Pharmacology of
human dopamine D3 receptor expressed in a mammalian
cell line: Comparison
with D2 receptor
Thus, high ALKBH1 activity can globally decrease the amount of proteins made in a
cell, as the researchers observed in a
human cell line with abnormally high levels of ALKBH1.
Establishment of a
human embryonic germ
cell line and comparison
with mouse and
human embryonic stem
cells.
Until now, scientists examining the causes and effects of insulin resistance have struggled
with a general lack of
human cell lines from tissues such as muscle, fat and liver that respond significantly to insulin, Kahn says.
To do so, they started
with a
human embryonic stem
cell line, which they chemically nudged to become
cells that form what's known as the primitive streak on the hollow ball of
cells of the early embryo.
TNA3: BPRC will offer access to its library of
human cell reporter assays for the identification of innate immune receptor - induced signalling cascades, including
human cell lines transfected
with PAMPs such as TLR and / or engineered to express luciferase in response to NF - kB or IFRE - mediated signal transduction, and
cell lines that provide insight into the intracellular signalling cascades of innate immune receptors.
In cultured
human cells and in rabbits
with implanted stents, Finn and colleagues showed that metformin augmented the effect of mTOR inhibitors on regrowth of the blood vessel
lining.
NeuroStemcell is focused on the identification and systematic comparison of progenitor
cell lines with the most favourable characteristics for mesDA and striatal GABAergic neuronal differentiation, generated either directly from
human embryonic stem (ES)
cells, from Neural Stem (NS)
cells derived from ES
cells or fetal brain, from induced Pluripotent Stem (iPS)
cells or from in vitro short - term expanded neural progenitors from ventral midbrain grown as neurospheres (VMN, Ventral Midbrain Neurospheres) 4, and perform rigorous and systematic testing of the most prominent candidate
cells in appropriate animals models.
The findings from this one
cell line are also inconsistent
with clinical data from preimplantation genetic diagnosis, further suggesting that the stem
cell assay may not wholly reflect what happens during
human development.
Human cord - blood T -
cells infected
with co-cultivation
with HTLV - positive T -
cell lines.
HAT sensitive derivative of CEM, a
human T -
cell line derived from the peripheral blood buffy coat of a four - year old Caucasian female
with acute lymphoblastic leaukemia.
Sphere - forming
cell subpopulations
with cancer stem
cell properties in
human hepatoma
cell lines.