Sentences with phrase «with immunofluorescence»

Not exact matches

An immunofluorescence image of a melanoma cell stained with antibodies against the cytoskeleton components indicate their invasion into surrounding matrix.

«When you're doing immunofluorescence, for instance, if you're using the wrong fixation process or fixative you could really get the wrong results even with a great antibody,» he says.

Immunofluorescence analysis of prostate cancer cells treated with 15mM flutamide, 35?

A large number of hippocampal neurons were stained with cyclin - dependent kinase 5 in the offspring from the ethanol - treated group (immunofluorescence, x 400).

Immunofluorescence microscopy was performed using a Zeiss Axioplan fluorescence microscope (Carl Zeiss, Inc., Thornwood, NY) equipped with a 100 - W Hg lamp and narrow band pass excitation filters (Chroma Technology Corp., Brattleboro, VT).

In total, more than 500 proteins were analyzed with two well - established methods used in most laboratories for biological studies; immunofluorescence (IF) and fluorescence protein (FP) tagging.

Cells were washed once with PBS and immunofluorescence was documented.

Immunofluorescence staining of HeLa cells with purified ab32518 at a working dilution of 1 in 50, counter-stained with DAPI.

All genes with Data reliability «Supported» in one or both of the two methods immunohistochemistry and immunofluorescence, or standard validation «Supported» for the Western blot application (assays using over-expression lysates not included) are classified as «Evidence at protein level».

(B) Immunofluorescence staining with antibodies against Sox2, Nestin, Olig2 and Tuj1.

(d — f) Immunofluorescence staining of HFF cells with the antibody against VIMENTIN.

Immunofluorescence detection of Abeta42 plaques and quantification of amyloid burden of brain tissue were measured and sections were analyzed with Image J (NIH) software.

Frozen sections of lung and colon tissues from wild - type mice and Slc6a14 − / − mice were also used for immunofluorescence analysis of Slc6a14 protein expression with an antibody raised against a mouse peptide sequence [17].

The HepG2 cells were treated with ActD for 6 h, and the p53 expression level was examined by immunofluorescence imaging.

Immunocytochemistry / Immunofluorescence analysis of human cardiac stem cells labeling Ki67 with ab16667 at 1/250 dilution.

For immunofluorescence, the cells were fixed in 4 % paraformaldehyde / PBS for 20 minutes, washed twice with PBS, and blocked with 4 % goat serum in PBS for 30 min, with all procedures performed at room temperature.

Subsequently, immunofluorescence labeling is performed with a rat - anti-BrdU primary antibody and a goat - anti-rat-Alexa488 secondary antibody to detect BrdU.

In later stages, the NLS - PCP - GFP fluorescent signal is reduced at the posterior pole and Oskar protein (blue) is detected by immunofluorescence, consistent with translation of a portion of the transcripts (see Halstead *, Lionnet *, Wilbertz *, Wippich * et al., Science 2015).

After binding, cells were washed once (carefully using a 200 μl pipette to remove the medium) with RPMI with 0.5 % BSA solution and adherent cells were analyzed by immunofluorescence microscopy or bright field microscopy for quantification.

All cases were analysed for IgA anti-gliadin (AGA) by an ELISA test and those with positive results were tested for IgA anti-endomysium antibody (EMA) using immunofluorescence.

Positive FHV - 1 test results (virus isolation, immunofluorescence techniques, and conventional PCR assays) do not differentiate subclinically infected cats from those with clinical disease as a result of FHV - 1 infection.

In cases of neonatal mortality, the diagnosis typically is made postmortem with virus isolation from fresh lung, liver, kidney, and spleen by cell culture techniques and subsequent identification by PCR and sequencing, transmission electron microscopy, immunofluorescence, or fluorescence in situ hybridization.