An Alexa Fluor ® 488 conjugate
with isotype specificity to the mouse antibody was used as a secondary antibody.
(E) Mice were treated beginning day 2 postinfection, and every 3 d thereafter
with isotype control, IFN - γ blockade only, TNF - α blockade only, or IFN - γ / TNF - α dual blockade following infection.
Not exact matches
Primary isolates that were incubated
with identical concentrations of FITC - and PE - conjugated
isotype standards were used to assess the level of background intensity.
In addition, rabbits only have one
isotype of IgG molecules
with more disulphide bonds.
IHC - P mouse tumor tissue (from lung)
with human cell line injected, some muscle tissue attached as well sees high background for human cellswith priamry Ab as well as
isotype ctrl, but also for muscle (does not contain any EGF) Ab: 1 ug /...
HL - 60 cells were stained
with 1 µg / mL ab14715 (blue) or an equal amount of an
isotype control antibody (red) and analyzed by flow cytometry.
After washing
with PBS and blocking for 30 minutes (IHC / ICC Blocking Buffer - Low Protein; eBioscience), cells were incubated
with anti - Oct ‑ 4 antibody (diluted 1:150; eBiosicence) or an IgG2a K
isotype control (diluted 1:150; eBiosicence) for one hour.
(C) Survival of gp33 - immunized mice
with LCMV infection following 30 d rest period compared
with control mice
with ST2 blockade or
isotype control Ab treatment.
LCMV - infected mice were injected i.p.
with 150 μg ST2 blocking Ab or
isotype control every other day beginning on day 2 postinfection (14).
Prf1 − / − mice were immunized against gp33 or
with control procedure, rested for 30 d, infected
with LCMV, and treated
with either IFN - γ blockade or
isotype control beginning day 2 postinfection, and every 3 d thereafter.
(B) gp33 - immunized and control mice were treated
with IFN - γ blockade or
isotype control Ab treatment.
Cells were stained
with STAT6 monoclonal antibody or mouse IgG1
isotype control antibody or without the primary antibody.
Inhibition of cell proliferation (B) of MCF7, MCF7 / HER2, or BT474 cells treated for 6 days
with F3 - IgG, trastuzumab, or
isotype control antibody.
An isotyping ELISA was performed by coating a 96 - well plate
with 1.25 ug / mL of the IgG1
Isotype Control Antibody and detecting
with Alexa Fluor conjugates specific to mouse IgG1, IgG2a, IgG2b, IgG3, IgM and heavy and light chains (H&L) of IgG.
Breast carcinoma cells (5 × 103 cells / well) were seeded into a 96 - well plate and treated
with trastuzumab,
isotype - matched control IgG, or F3 - IgG at concentrations ranging from 0.01 to 10 µg / mL.