Sentences with phrase «with isotype control»

(E) Mice were treated beginning day 2 postinfection, and every 3 d thereafter with isotype control, IFN - γ blockade only, TNF - α blockade only, or IFN - γ / TNF - α dual blockade following infection.

Not exact matches

HL - 60 cells were stained with 1 µg / mL ab14715 (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.

After washing with PBS and blocking for 30 minutes (IHC / ICC Blocking Buffer - Low Protein; eBioscience), cells were incubated with anti - Oct ‑ 4 antibody (diluted 1:150; eBiosicence) or an IgG2a K isotype control (diluted 1:150; eBiosicence) for one hour.

(C) Survival of gp33 - immunized mice with LCMV infection following 30 d rest period compared with control mice with ST2 blockade or isotype control Ab treatment.

LCMV - infected mice were injected i.p. with 150 μg ST2 blocking Ab or isotype control every other day beginning on day 2 postinfection (14).

Prf1 − / − mice were immunized against gp33 or with control procedure, rested for 30 d, infected with LCMV, and treated with either IFN - γ blockade or isotype control beginning day 2 postinfection, and every 3 d thereafter.

(B) gp33 - immunized and control mice were treated with IFN - γ blockade or isotype control Ab treatment.

Cells were stained with STAT6 monoclonal antibody or mouse IgG1 isotype control antibody or without the primary antibody.

Inhibition of cell proliferation (B) of MCF7, MCF7 / HER2, or BT474 cells treated for 6 days with F3 - IgG, trastuzumab, or isotype control antibody.

An isotyping ELISA was performed by coating a 96 - well plate with 1.25 ug / mL of the IgG1 Isotype Control Antibody and detecting with Alexa Fluor conjugates specific to mouse IgG1, IgG2a, IgG2b, IgG3, IgM and heavy and light chains (H&L) of IgG.

Breast carcinoma cells (5 × 103 cells / well) were seeded into a 96 - well plate and treated with trastuzumab, isotype - matched control IgG, or F3 - IgG at concentrations ranging from 0.01 to 10 µg / mL.