Sentences with phrase «with laminin»

Cells were plated either on glass coverslips (40,000 cells / coverslip) coated with poly - L lysine and laminin or six - well plates (106 cells / well) coated with laminin alone.

Representative images of EDL and TA muscles stained with laminin / DAPI and H&E demonstrate these morphological changes (Figure 6, D and H).

The new study by the Inagaki lab showed that neurons will produce four times more traction force when placed on adhesive substrates coated with laminin than those without laminin, assuring the axons reach their final destination.

The ratio of grip state increased with laminin,» said Inagaki.

As expected, additional experiments by Abe showed that the F1 - actin flow was dependent on L1 - CAM interactions with laminin.

«When animals with a laminin - α2 defect express the two linkers, there was a significant improvement in muscle structure and force and an increase in body weight.

Not exact matches

Two labs also detected the proteins laminin and elastin with antibody tests, although mass spectrometry failed to turn up sequences for these proteins.

Quantification of total fiber number from laminin / DAPI - stained sections revealed a 28 % greater number of total muscle fibers in EDL muscles and a 29 % greater number of total muscle fibers in TA muscles from IL - 15Rα — KO mice compared with B6129 controls (Figure 6, A and E).

(E) The total number of muscle fibers counted in laminin - stained TA muscle sections from IL - 15Ra — KO mice was greater compared with B6129 control.

Cells were resuspended in HEPES buffer containing the following (in mm): 136 NaCl, 5.6 KCl, 1 MgCl2, 2.2 CaCl2, 11 glucose, 10 HEPES; pH adjusted to 7.4 with NaOH before plating on poly - d - lysine (0.02 mg / ml; BD Bioscience) and laminin (0.02 mg / ml; Sigma - Aldrich)- coated coverslips.

(A) The total number of muscle fibers in laminin - stained EDL muscle sections from IL - 15Ra — KO mice was greater compared with B6129 control.

Next - generation «designer matrices» such as hybrid polyethylene glycol hydrogels or microengineered collagen scaffolds, combined with a well - defined set of laminins, may better fulfill the niche requirements of organoids and may be customized for a specific type of tissue / organoid (2,6).

Laminin - 521 induce fast migration of the cells and laminin isoform specific activation of PI3K / Akt pathway through interaction with α6β1 inLaminin - 521 induce fast migration of the cells and laminin isoform specific activation of PI3K / Akt pathway through interaction with α6β1 inlaminin isoform specific activation of PI3K / Akt pathway through interaction with α6β1 integrin.

LAMscreen is a kit with all our currently available laminin isoforms so that you can easily screen the best laminins for different cell types.

Cells were grown on polyornithine and laminin coated dishes and passaged 1:2 with PBS + + (PBS with 1 % BSA).

and cultured in 5 % O2 / 7 % CO2 in Bottenstein - Sato F12 medium with 10 ng / ml human recombinant basic fibroblast growth factor (Peprotech) on a substrate of 1 µg / cm2 fibronectin (Chemicon) and 0.5 µg / cm2 laminin (Invitrogen).

Rapid spreading and mature hemidesmosome formation in HaCaT Keratinocytes induced by incubation with soluble laminin - 5r.

SCR055, Millipore, Inc) in 24 - well culture dishes coated with poly - L - ornithine (20 µg / ml) and laminin (5 µg / ml).