Sentences with phrase «with lentivirus»

BP were transduced with lentivirus as described below before injection.

CSCs were prepared from 231BrM and CN34BrM cells that were infected with lentivirus carrying with or without miR -7-2, and they were transplanted into nude mice through intracardiac injection followed by monitoring metastatic tumor growth in the brain.

B, CSCs isolated from 231BrM infected with lentivirus of miR7 or KLF4 expression plasmids were seeded on top of mouse brain endothelial cells or Matrigel.

Not exact matches

Its product, the TRIM5 protein, interacts directly with the outer shell of lentivirus particles after they enter the host cells and prevents the virus from multiplying there.

«We tried for a long time to introduce Cas9 with plasmids or lentiviruses, and then to express separately the single - guide RNA in the cell,» Schumann said.

B, CSCs were isolated from 231BrM infected with KLF4 or control lentivirus and cultured in low - attachment plates.

D, the same primary breast cancer cells infected with KLF4 or control lentivirus were cultured in low - attachment plates and the number of mammospheres was counted after 10 days.

The tumor cells were dissociated and the cells were infected with KLF4 lentivirus and cultured in low - attachment plates.

C, primary breast cancer cells isolated from patients with advanced cancer were directly infected with KLF4 or control lentivirus, and the CSC population (CD24 − CD44 + ESA +) was measured by FACS after culturing the cells for 72 hours in low - attachment plates.

Dr. Sonntag studies this concept on the molecular and cellular level using a translational research approach that integrates the analysis of human material, such as postmortem brains, primary cell systems, and neural cell populations generated from patients» - or healthy individuals» - derived induced pluripotent stem cells (iPSC), or induced neurons (iNs), in combination with molecular, biochemistry, and lentivirus - mediated gene - engineering technologies.

A lentiviral vector with the same modifier, Fuw - dCas9 - Tet1CD, is available from Rudolf Jaenisch's lab in plasmid form or as ready - to - use lentivirus.

For lentiviral expression, Fuw - dCas9 - Dnmt3a and Fuw - dCas9 - Dnmt3a - P2A - tagBFP are available from Rudolf Jaenisch's lab, with the former also available as ready - to - use lentivirus.

We began with only a few inventory items offered domestically, but by the end of 2016, we expanded our viral inventory to 25 lentiviruses and 25 AAVs.

Human embryonic kidney (HEK) 293T cells, a packaging cell line for lentivirus production, and the preosteoclast cell line RAW264.7 (ATCC) were maintained in DMEM supplemented with 10 % FBS and antibiotics.

DNA fingerprinting analysis at 16 independent loci indicated that both iPSCs generated by lentivirus infection (iPSC colony 19) and by transient transfection with episomal vectors (iPSC colony 1) and the original human fetal NSCs (ReNCell VM) shared all alleles investigated and were different from commonly available hESC lines.

Researchers used a lentivirus to infuse a normal copy of the ABCD1 gene into the bone marrow of boys with cerebral adrenoleukodystrophy (ALD), and the corrected protein stopped disease progression.

We found that the percentage of the EdU - positive cells in the GFP - positive organoids was significantly reduced when the human BCSCs were infected with the anti-miR-142-3p-expressing lentivirus (Figure 4C).

And we confirmed that the growth of the tumors formed by the human BCSCs transfected with the anti-miR-142-expressing lentivirus was significantly slower than those of the control tumors formed by the control lentivirus transfected BCSCs (Major points raised by the editors and the reviewers # 3) These data suggest that the regulation of APC and the Wnt signaling is at least one of the important pathways targeted by miR - 142 in human breast cancer cells and BCSCs.

We further confirmed that the protein level of APC was elevated in the human breast cancer xenograft cells transfected with the anti-miR-142-3p lentivirus (Figure 7B).

To confirm that these findings are applicable to the human BCSCs, we infected the human breast cancer cells with the anti-miR-142-3p-expressing lentiviruses and evaluated the ability to form the organoids derived from the human BCSCs.

To evaluate the role of miR - 142 in the growth of human breast cancers initiated by the human BCSCs, we infected human BCSCs isolated from a patient - derived human breast cancer xenograft (PDX) with the anti-miR-142-3p-expressing lentivirus or the control lentivirus.

Mammary cells infected with the miR -150-expressing lentivirus formed a hyperplastic mammary tree with extremely increased branching and thick mammary ducts (Figure 6A, C).

In contrast, mammary cells infected with the miR - 142 lentivirus formed disorganized structures with multiple layers of cells (Figure 6A, B).

We confirmed that the protein level of APC was elevated and the expression of miR - 150 was decreased in the human breast cancer xenograft cells transfected with the anti-miR-142-3p lentivirus (Figure 5E and Figure 7B).