Expression of VR and OR genes in RNAseq compared
with microarray expression data.
The correlation is considerably higher with the RNAseq data (Pearson correlation r2 = 0.81 for the VNO and 0.9 for the OM) than
with the microarray values (Pearson correlation r2 = 0.58 for the VNO and 0.52 for the OM), indicating that RNAseq is better suited for transcriptome profiling in the olfactory system.
As a research associate in the pathology labs of Yale School of Medicine, Mrs. Canosa has worked extensively
with microarray expression profiling, genotyping, and DNA sequencing.
Melton and colleagues are now doing large scale screening
with microarrays to track gene activity as cells develop and discover gene products that move cells along the proper paths.
With all of the SNP genotypes in hand, researchers could assess gene expression — initially
with microarrays, and later with RNA - seq — and then correlate it with genetic variation.
Combining our in vitro assay
with microarrays analyses, we have uncovered previously unknown effectors and target genes of Wnt.
Not exact matches
Natera uses a SNP
microarray from Ilumina
with a proprietary technology called Parental Support ™, which tests all 24 chromosomes and delivers results that are typically > 99 % accurate.
Larger biomarker signatures can be detected
with technology from CDI Laboratories, which offers
microarrays of functional human proteins (over 20,000 on a single array) to test the antibodies present in human liquid biopsy samples, such as blood, serum, plasma, CSF, or tissue lysates.
Eager to sort through the pile of curriculum vitae's on his desk, Rudnicki is particularly looking for molecular geneticists working on different stem cell systems; scientists
with genomics bents like proteomics,
microarray analysis, and bioinformatics; and a couple of clinician scientists.
The technology for genome - wide profiling using
microarrays was newly available, and
with it came tremendous excitement, parallel to the mapping of the human genome.
This study, published in the journal
Microarrays, shows that lack of SOST in the bone microenvironment promotes the expression of many genes associated
with cell migration and / or invasion, including long non-coding RNA MALAT1 in prostate cancer, suggesting that SOST has an inhibitory effect on prostate cancer invasion.
ONE OF THE FIRST THINGS MIKOVITS DID was to employ a
microarray — a small tray seeded
with DNA from nearly every known virus — to flag viral DNA in human white blood cells.
To date, we have produced over 40,000
microarrays,
with 20,000 shipped in the last year alone.
This year we will be hosting our 3rd Annual International
Microarray Symposium, a 3 - day event that allows microarray users to interact with experts in
Microarray Symposium, a 3 - day event that allows
microarray users to interact with experts in
microarray users to interact
with experts in the field.
He had spent half of his graduate years working
with one small DNA
microarray data set, but Millennium Pharmaceuticals in nearby Cambridge was «printing
microarrays to beat the band; they had hundreds.»
To help identify genes and signaling pathways that may be influenced by zinc, Knoell and his team performed a genome - wide
microarray analysis of lung tissue taken from zinc - deficient mice
with sepsis.
There is a large sequencing group
with a battery of sequencers, a
microarray group, a proteomics group, and several groups being formed.
To understand why only some motor neurons are vulnerable to ALS, the researchers used DNA
microarray profiling to compare the activity of tens of thousands of genes in neurons that resist ALS (oculomotor neurons / eye movement and Onuf's nuclei / continence)
with neurons affected by ALS (lumbar 5 spinal neurons / leg movement).
Stephen W. Scherer, Ph.D., of the Hospital for Sick Children, Toronto, and colleagues performed chromosomal
microarray analysis (CMA) and whole - exome sequencing (WES) in a group of 258 unrelated children
with ASD to determine the molecular diagnostic yield (the percentage of subjects
with a genetic alteration [mutation] that may contribute to the features of autism spectrum disorder) of these tests.
In essence,
microarrays are fingernail - sized chips embedded
with snippets of known DNA.
The expression rates of three macrophage - specific genes (Emr1, Cd68, and Csf1) that correlated
with body mass in our
microarray studies, an adipocyte - specific gene (Acrp30), and proinflammatory genes (Tnfa, Nos2, Il6) were determined by quantitative RT - PCR.
Classical pathological, bacteriological, serological, molecular, histological and electron microscopical methods were combined
with high throughput
microarray and next generation sequencing methods to undertake the most extensive and exhaustive evaluation of the cause of death of any zoo animal to date.
To identify transcriptional patterns that correlate
with body mass, we used oligonucleotide
microarrays to catalogue gene expression levels in the parametrial or epididymal adipose tissue from two dozen mice whose body mass and adiposity varied due to diet, sex, and mutations in genes affecting energy homeostasis.
These are among the genes whose expression in our
microarray expression data set correlated positively
with body mass and adipocyte size.
The Monash BDI researchers worked in collaboration
with Canadian - based company Kinexus, and used an antibody
microarray to simultaneously investigate hundreds of factors involved in cell signalling that were modulated by HCV replication, including human protein kinases.
Band DNA was labeled
with Cy5 and hybridized to
microarrays with Cy3 - labeled whole genome DNA.
In this study, we employed a pan-viral
microarray assay, the Virochip, to identify a novel adenovirus associated
with a fulminant pneumonia outbreak in a colony of New World titi monkeys.
The advent of
microarray technology,
with its capacity to monitor the expression of thousands of genes simultaneously, has provided a novel opportunity to identify individual genes, groups of genes, and related «gene families» associated
with a given biologic process.
Part of this effort involved discovery, through transcriptomic evidence (whole - genome
microarrays), that up - regulation of the native fadM thioesterase in E. coli was correlated
with enhanced methyl ketone titers.
To pursue this multi-dimensional research
with a holistic perspective, we apply next - generation sequencing,
microarrays, proteomics, and functional profiling of mutant strains.
Services provided by EMBL Heidelberg include the Core Facilities which provide internal users, collaborators and visitors
with access to excellent facilities for high - throughput analysis including
microarrays, protein production, mass spectrometry, DNA sequencing, advanced light and electron microscopes and chemical genomics.
The
microarray data
with the description of the experimental design are deposited under GEO number GSE86885.
After initially applying the genomic SNP chromosomal
microarray only in fetuses
with ultrasound abnormalities, since July 2012, we routinely performed the SNP array also in cases without fetal ultrasound anomalies where invasive prenatal diagnosis was indicated.
We work very closely
with the Sequencing and
Microarray Facility (SMF) and the DNA / RNA samples extracted from us can be submitted directly from our core to SMF across the hallway.
This will be achieved by exposing the coral to seawater
with high calcium concentrations to induce an alteration in gene expression profiles, identified using a DNA
microarray analysis.
Total RNA was labeled and hybridized against custom S. pistillata
microarray; an Agilent two - color gene expression
microarray platform
with 8 × 15 K probe per slide;
microarray samples included four SW +100 and four SW (control) samples for each time point (11:00 and 23:00).
The facility collaborates
with various research projects using flow cytometric techniques to isolate and purify cells and chromosomes for structural and functional studies, sequencing,
microarrays as well as fluorescence insitu hybridisation (FISH) experiment.
Along
with the cellular genes emphasized from the
microarray analysis, two genes that are suspected to be involved in the scleractinian coral calcification process were tested for expression (Figs. 5A and 5B).
In addition, analysis of the
microarray results obtained
with RNA from the optimized protocol showed that, as expected, the genes involved in methane production (methanogenesis) were among the most highly expressed genes in M. barkeri.
Comparison of their genomes, integrated
with proteomic and
microarray data,
with the genomes of Plasmodium falciparum and Plasmodium yoelii revealed a conserved core of 4500 Plasmodium genes in the central regions of the 14 chromosomes and highlighted genes evolving rapidly because of stage - specific selective pressures.
Microarray Analysis: Anti-Human SMAD4 (clone R516.2.1 D12) specificity was evaluated using CDI HuProt ™ Human Proteome Microarray and subsequently analyzed with GenePix Pro Image Acquisition and Analysis Software, the benchmark tool for the acquisition and analysis of microarr
Microarray Analysis: Anti-Human SMAD4 (clone R516.2.1 D12) specificity was evaluated using CDI HuProt ™ Human Proteome
Microarray and subsequently analyzed with GenePix Pro Image Acquisition and Analysis Software, the benchmark tool for the acquisition and analysis of microarr
Microarray and subsequently analyzed
with GenePix Pro Image Acquisition and Analysis Software, the benchmark tool for the acquisition and analysis of
microarraymicroarray images.
Microarray Analysis: Test antibody specificity was evaulated using CDI HuProt ™ Human Proteome Microarray and subsequently analyzed with GenePix Pro Image Acquisition and Analysis Software, the benchmark tool for the acquisition and analysis of microarr
Microarray Analysis: Test antibody specificity was evaulated using CDI HuProt ™ Human Proteome
Microarray and subsequently analyzed with GenePix Pro Image Acquisition and Analysis Software, the benchmark tool for the acquisition and analysis of microarr
Microarray and subsequently analyzed
with GenePix Pro Image Acquisition and Analysis Software, the benchmark tool for the acquisition and analysis of
microarraymicroarray images.
At Berkeley, she developed computationally intensive statistical methods for the analysis of
microarray data
with applications in cancer biology.
Microarray Analysis: Test antibody specificity was evaulated using CDI HuProt ™ Human Proteome Microarray (~ 75 % of the human proteome) and subseqently analyzed with GenePix Pro Image Acquisition and Analysis Software, the benchmark tool for the acquisition and analysis of microarr
Microarray Analysis: Test antibody specificity was evaulated using CDI HuProt ™ Human Proteome
Microarray (~ 75 % of the human proteome) and subseqently analyzed with GenePix Pro Image Acquisition and Analysis Software, the benchmark tool for the acquisition and analysis of microarr
Microarray (~ 75 % of the human proteome) and subseqently analyzed
with GenePix Pro Image Acquisition and Analysis Software, the benchmark tool for the acquisition and analysis of
microarraymicroarray images.
Under the stewardship of Christine Armour, the working group, consisting of members from the CCMG Clinical Practice and Laboratory Practice committees
with representatives from the SOGC, have updated the 2001 Canadian guidelines for use of chromosomal
microarray analysis (CMA).
Furthermore, high ABL1 mRNA was associated
with bone metastasis in a
microarray data set reporting organ - specific metastasis (Fig. 1F)(18).
Proteomic analysis to profile protein abundance resulted in the identification and relative quantification for 912 proteins
with two or more unique peptides and 86 proteins
with significant abundance changes after treatment
with the neurotoxins, while
microarray analyses to profile gene expression revealed 181 genes
with significant changes in mRNA after treatment.
Microarray Analysis: Test antibody specificity was evaulated using CDI HuProt ™ Human Proteome Microarray (19,394 human proteins) and subseqently analyzed with GenePix Pro Image Acquisition and Analysis Software, the benchmark tool for the acquisition and analysis of microarr
Microarray Analysis: Test antibody specificity was evaulated using CDI HuProt ™ Human Proteome
Microarray (19,394 human proteins) and subseqently analyzed with GenePix Pro Image Acquisition and Analysis Software, the benchmark tool for the acquisition and analysis of microarr
Microarray (19,394 human proteins) and subseqently analyzed
with GenePix Pro Image Acquisition and Analysis Software, the benchmark tool for the acquisition and analysis of
microarraymicroarray images.
CDI HuProt
microarrays are currently being employed in an NIH grant to produce antibodies
with absolute specificity to all human transcription factors.
We also performed
microarray analysis of PyMT - Slc6a14 + / + and PyMT - Slc6a14 − / − mammary tumours to evaluate the expression of amino acid transporters without any biased preconception of the identity of the transporters that might be subject to changes in expression in association
with Slc6a14 deletion.