Sentences with phrase «with midgut»

Not exact matches

When food exits the pouch, it shoots straight into her midgut, continuing largely undigested until it reaches the Y junction and combines with the acidic brew of stomach juices.

Mcf1 treated caterpillars show rapid loss of body turgor (the «floppy» phenotype) within 12 hours, associated with massive apoptosis of the midgut epithelium.

Here, tick midguts were pooled per animal, pelleted, washed with 5 mL HBSS and then resuspended in HBSS in a final volume of 0.25 mL for mouse injections.

Standard PCR and RT - PCR for ospA, ospC and flaB were also performed with either SCID mouse tissues or XT midguts using primers described elsewhere [35].

In addition to staining the midgut contents with FITC - labeled polyclonal anti-Borrelia species antibody, we washed and re-stained this set and stained the second set of xenodiagnostic tick (XT) samples with an anti-OspA monoclonal antibody (CB10, obtained from J. Benach [41]-RRB-, followed by anti-mouse IgG - Alexa 488 (Molecular Probes).

These data demonstrated that OspC was not necessary for spirochetes to colonize the tick midgut but that mice remained refractory to infection with the ospC mutant, even by tick challenge.

(A) IFA of midgut tissues from partially fed nymphal ticks infected with B31 - A3 WT, ospC7 mutant, or ospC7 / ospC +4 complemented B. burgdorferi clones.

We injected four mice with homogenized midguts from partially fed, ospC7 - infected nymphs to determine whether the ospC mutant was infectious when obtained directly from a feeding tick.