Sentences with phrase «with model membranes»

«Even though we only demonstrated the technique with model membranes and single cells, this technique is translatable to biological tissue,» said Brasselet.

Not exact matches

The researchers have also developed an analogous model that works for calcium interactions with phospholipids at the cell membrane.

Here, we present experiments using a reconstituted fusion system that suggest a simple model in which the complexin accessory helix forms an alternative four - helix bundle with the target - SNARE near the membrane, preventing the vesicle - SNARE from completing its zippering.

Interactions of these proteins with the outer membrane in vivo and in vitro are well known, allowing for direct comparisons with the synthetic model.

The researchers began by creating large membrane models with embedded and dyed channel proteins; they then put them on a glass surface and measured them using a single - molecule measuring method known as fluorescence correlation spectroscopy.

Modeling revealed that tilting of the PHDs to conform with membrane deformations creates the low - energy pathway for hemifission.

Using whole - cell path clamp, both dSPNs and iSPNs from Q175 6 - month old mice showed elevated membrane resistance and reduced rheobase current, consistent with previous reports of SPN hyperexcitability in mouse models of HD.

The Evaluation of a Biphasic Osteochondral Implant Coupled with an Electrospun Membrane in a Large Animal Model.

With this series of protocols the BCL allows construction of backbone models for large and membrane proteins from the primary sequence.

In particular, an experimental study on the permeability of prebiotic vesicle membranes composed of binary lipid mixtures allows us to construct a semi-empirical model where protocells are able to reproduce and undergo an evolutionary process based on their coupling with an internal chemistry that supports lipid synthesis.

For example, KBs were recently reported to act as neuroprotective agents by raising ATP levels and reducing the production of reactive oxygen species in neurological tissues, 80 together with increased mitochondrial biogenesis, which may help to enhance the regulation of synaptic function.80 Moreover, the increased synthesis of polyunsaturated fatty acids stimulated by a KD may have a role in the regulation of neuronal membrane excitability: it has been demonstrated, for example, that polyunsaturated fatty acids modulate the excitability of neurons by blocking voltage-gated sodium channels.81 Another possibility is that by reducing glucose metabolism, ketogenic diets may activate anticonvulsant mechanisms, as has been reported in a rat model.82 In addition, caloric restriction per se has been suggested to exert neuroprotective effects, including improved mitochondrial function, decreased oxidative stress and apoptosis, and inhibition of proinflammatory mediators, such as the cytokines tumour necrosis factor - α and interleukins.83 Although promising data have been collected (see below), at the present time the real clinical benefits of ketogenic diets in most neurological diseases remain largely speculative and uncertain, with the significant exception of its use in the treatment of convulsion diseases.