Despite the presumed virulence of the strain — experiments
with mouse lungs showed it produces 1000 times more bacteria in infected cells than do standard varieties — Valway says the number of TB cases that developed were kept in line with other typical outbreaks, which «shows that doing good contact investigations is important and preventative therapy works.»
Not exact matches
«Indeed, in a second tumor model of metastatic breast cancer, we demonstrated that
mice treated
with the EphA2 - targeting paclitaxel conjugate presented nearly no
lung metastases, while a large numbers of lesions were observed in both untreated
mice and in
mice treated
with just paclitaxel.»
When
mice that had been implanted
with human
lung tumours inhaled these bubbles — which also contained molecules that suppress drug resistance — their tumours shrank.
Specifically, the researchers found that a peptide, called STAT6 - IP, when delivered to the
lungs of neonatal
mice at the time of first RSV exposure reduces the development of allergic - type
lung inflammation and airway hyperresponsiveness («twitchy» airways) in
mice when they are «re-challenged»
with RSV as young adults.
To their surprise, they found that these Th17 - deficient
mice experienced far more aggressive cancer growth than normal
mice —
with the cancer completely smothering the
lungs within 16 days.
In the Cell study, Dr. Massagué,
with Fellow Manuel Valiente, PhD, and other team members, found that in
mouse models of breast and
lung cancer — two tumor types that often spread to the brain — many cancer cells that enter the brain are killed by astrocytes.
The scientists conclude that in
mice «well - timed treatment
with rIL - 27 improved
lung injury and accelerated recovery without affecting viral clearance» and continue «these data demonstrate that IL - 27 has a unique role in controlling immunopathology without impacting on host defense, and might therefore represent a promising candidate for immunomodulatory therapy of viral pneumonia.»
When the researchers examined
mice with disrupted IL - 27 function, they found that they were more likely than normal
mice likely to die when infected
with the virus, and that they died as a consequence of rampant
lung inflammation.
Brueckner's group looked for gene defects in two strains of
mice with situs inversus, a mutation in which the heart,
lungs, and other organs are inverted, like a mirror image.
Compared to a control (left), epalrestat treatment (right) reduces the number of metastatic tumors (arrowheads) in the
lungs of
mice injected
with human basal - like breast cancer cells.
«This was associated
with an unexpected accumulation of T - cells in the
lungs of infected
mice that lack STIM1.»
All of these findings were supplemented
with several other experiments that were designed to learn how CHI3L1 interacts
with other cells involved in the tissue repair response in both human and
mouse lungs.
Three days later, the scientists discovered that although the
mice had cleared the bacteria from their livers,
lungs, and spleens, the tumorous tissue in their colons was crawling
with Salmonella.
Mice treated
with the combination had almost 40,000 times fewer bacteria in their
lungs.
In addition, low levels of PD1 in the
lungs of influenza - virus - infected
mice were associated
with severe infection and highly pathogenic viruses, such as H5N1.
To help identify genes and signaling pathways that may be influenced by zinc, Knoell and his team performed a genome - wide microarray analysis of
lung tissue taken from zinc - deficient
mice with sepsis.
The researchers also found that nanorods targeted to
lung tissue in
mice accumulated at a rate that was two-fold over nanospheres engineered
with the same targeting antibody.
Both the number and activity of osteoblasts — cells that produce and reshape bone tissue — were increased within the bone marrow of
mice with lung tumors compared
with cancer - free animals; and reducing the number of osteoblasts in
mice not only limited neutrophil infiltration of tumors but also interrupted tumor progression.
The cells exhibited many functions associated
with tumor progression; their presence within
mouse tumors substantially accelerated cancer growth, and in human
lung tumors, a SiglecFhigh neutrophil signature was associated
with poor patient survival.
Adult male
mice also started off
with fewer of the lymphoid cells in the
lungs than adult females, the researchers found: Female
mice had about 1 1/2 times as many of these immune cells as males.
In a race
with other scientists, Scottish researchers have bred
mice with an altered gene which displays all the key features of cystic fibrosis, such as
lung disease and digestive problems.
Finally, the researchers showed in tests
with mice that their nanoparticle iNOP - 7DS can be delivered effectively to the liver, spleen, and
lung; and it suppressed the level of messenger RNA involved in the expression of the ApoB gene.
Mice injected
with breast cancer cells making lots of a long strand of RNA called HOTAIR developed 10 times as many
lung tumors as controls (left) did.
When infected
with TB,
mice lacking Smurf1 had higher bacterial loads, increased
lung inflammation, and accelerated mortality compared to
mice with normal Smurf1 activity, Dr. Shiloh said.
Mice infected
with a resistant strain of tuberculosis that were treated
with both SMARt - 420 and ethionamide also showed a significantly reduced bacterial load in their
lungs three weeks after infection compared to controls, the authors report.
In multiple trials of
mice with both active and chronic TB infections, researchers report that one version of the new drug — an analog known as 1599 — was as good as or better than current TB drugs at reducing levels of the bacteria in the
lungs of
mice.
Here, we show that in germ - free (GF)
mice, invariant natural killer T (iNKT) cells accumulate in the colonic lamina propria and
lung, resulting in increased morbidity in models of IBD and allergic asthma as compared
with that of specific pathogen - free
mice.
Female
mice and male
mice that had been treated
with estrogen were able to clear the bacteria from their
lungs more rapidly than normal male
mice.
This experiment uses quantitative PCR to detect the expression level of POSTN in CD34 + / CD31 − pulmonary fibroblasts, CD31 + endothelial cells, and CD45 + immune cells isolated from
lungs of
mice with macrometastases, which is a replication of the experiment reported in Figure 2H.
Immediately after counting macrometastasis, use the first six
lungs identified from MMTV - PyMT + / tg; Postn + / + female
mice that are positive
with metastatic disease for further analysis (Protocol 2).
The first experiment is to measure metastatic
lung cancer in MMTV - PyMT transgenic
mice crossed
with the POSTN Knock out
mice, and controls,
with focus on the role of periostin in metastatic progression.
When the researchers administered daily doses of BCX to
mice before and after they injected the rodents
with a nicotine - derived carcinogen, they found that the
mice treated
with BCX had fewer
lung tumors than those that got no BCX.
As a result, the
mice had twice as many cancer cells zipping through their bloodstream and in their
lungs compared
with mice not treated
with paclitaxel.
IHC - P
mouse tumor tissue (from
lung)
with human cell line injected, some muscle tissue attached as well sees high background for human cellswith priamry Ab as well as isotype ctrl, but also for muscle (does not contain any EGF) Ab: 1 ug /...
Salk scientists found that the diabetes drug phenformin was effective at reducing tumor size in
mice with lung cancer.
Oral administration of mebendazole in
mice elicited a strong antitumor effect in a subcutaneous model and reduced lesions in experimentally induced
lung metastasis without any toxicity when compared
with paclitaxel - treated
mice [9,20].
The dual - drug therapy —
with analogs already in use for other diseases — doubled the survival rate of
mice with lung cancer and halted cancer in pancreatic cells.
In recent years, researchers have developed so - called «senolytic» drugs that wipe out senescent cells in aging
mice and
mouse models of age - related disease, exploiting the high dependence of these cells on specific biochemical survival pathways.9, 10 In these studies, senolytic drugs have restored exercise capacity9 and formation of new blood and immune precursor cells11 in aging
mice to near youthful norms, and prevented or treated
mouse models of diseases of aging like osteoarthritis, 12 fibrotic
lung disease, 13 hair loss, 14 atherosclerosis, 15,16 and age - related diseases of the heart itself.9 UNITY Biotechnology is leading a growing charge toward the clinic,
with human clinical trials expected to begin in 2019.
Representative photomicrographs of
lung tissue from unvaccinated unchallenged
mice (normal) and from Balb / c
mice two days after challenge
with SARS - CoV that had previously been given PBS only (no vaccine) or live virus.
On day 56, five
mice from each group were sacrificed for assessing serum neutralizing antibody titers and
lung histopathology; the remaining seven or eight
mice in each group were challenged
with 106TCID50 / 60 µl of SARS - CoV intranasally (IN).
The concern for an occurrence of
lung immunopathology on challenge of
mice vaccinated
with an inactivated virus vaccine, as reported by Haagmans, et al. for ferrets and nonhuman primates, was seen by us after challenge of
mice vaccinated
with a SARS VLP vaccine [20].
When they blocked IKK2 activity in the
mice with lung cancer, the
mice had smaller tumors and lived longer, suggesting that the enzyme is necessary for NF - KB to stimulate tumor growth.
Representative photomicrographs of
lung tissue two days after challenge of Balb / c
mice with SARS - CoV that had previously been given a SARS - CoV vaccine.
The immunopathology in all experiments in the present study occurred in the absence of detectable virus in
lungs of
mice two days after challenge
with infectious virus.
Mean
lung cellular infiltration / lesion pathology and percent eosinophils in infiltrates for each vaccine dosage group for each
mouse strain (Balb / c or C57BL / 6) two days after challenge
with SARS - CoV.
Similar observations were noted at the Health Protection Agency (HPA) in the UK, where different morphotypes were observed from
lung samples in
mice infected
with Bps K96243.
«We developed a new method of initiating
lung cancer in
mice, which has properties associated
with human
lung cancer, and used this model to identify the role of this enzyme in cancer proliferation.
We found no change in the total number of ILCs (Lin - CD45.2 + Thy1.2 + CD127 +) in the spleens,
lungs or livers of toxin treated
mice compared to
mice that were injected
with vehicle control (Fig 6A and S6A Fig).
Frozen sections of
lung and colon tissues from wild - type
mice and Slc6a14 − / −
mice were also used for immunofluorescence analysis of Slc6a14 protein expression
with an antibody raised against a
mouse peptide sequence [17].
This strain showed a highly virulent phenotype,
with rapid multiplication during the early stage of infection in a murine model of TB
with excessive
lung inflammation, and infection
with this strain resulted in more rapid death of
mice than infection
with the reference H37Rv strain.