Sentences with phrase «with oligo»
Reverse transcription was performed on 1 μg of total RNA with an oligo (dT) primer (Roche) and M - MLV RT (Invitrogen).
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RNA was DNase treated (Roche) and cDNA was reverse transcribed using Transcriptor (Roche) with oligo dT as the primer.
Specifically, reverse transcription of 5 µg total RNA primed with an oligo (dT) primer bearing a T7 promoter was followed by in vitro transcription, generating multiple antisense copies of each mRNA in the sample (Ambion, Austin, TX).
cDNA synthesized by reverse transcription of 1 µg RNA primed with oligo (dT) and random 9 - mer primers was used as the substrate for quantitation of mRNA expression levels by quantitative RT — PCR in the presence of SYBR ® Green (Stratagene, La Jolla, CA).
Importantly, this requires a separate assay: usually, a custom array with oligos designed for each exon in every panel gene.
Not exact matches
After tinkering with several ingredients, they struck gold with a mixture of two chemicals, oligo (e-caprolactone) dimethacrylate and n - butyl acrylate.
Levels of APOE protein dropped by about half in mice given the APOE compound as compared with those that received the placebo oligo or saltwater.
Wagner conducts the experiment in parallel with me, and his plasmid properly incorporates the oligo that will guide Cas9 to its target.
With SapTrap, the user first designs either oligos or synthetic DNA for the desired gRNA target sequence, as well as the 5» and 3» homology arm repair template (Fig. 1, Step 1).
They reiterate that all of the following foods can precipate digestive symptoms in IBS patients: dairy, foods which are high in FODMAPS (fermentable oligo -, di -, monosaccharides, and polyols), high fat and spicy foods, foods with high levels of biogenic amines, i.e. histamine (such as soy), lectins (present in beans), and preservatives, such as benzoic acid and sulfites.
We put him on a gluten - free diet and a low - fermentable foods diet (also known as low FODMAPS or Fermentable Oligo - Di - Monosaccharides and Polyols), along with healing bone marrow broth and supportive nutrients.
Fructose and lactose are considered FODMAPs (fermentable oligo -, di - and monosaccharides and polyols), which can cause abdominal bloating, excessive gas (flatulence), loose stools or diarrhea in individuals with fructose malabsorption, lactose intolerance and irritable bowel syndrome (IBS).
FOS is fermentable by intestinal bacteria, so it belong to FODMAPs (Fermentable, Oligo -, Di - and Monosaccharides and Polyols), which can, even in small amounts, trigger excessive gas and diarrhea in sensitive individuals, mainly in those with fructose malabsorption and irritable bowel syndrome (IBS).
La Sal restaurant offers avante garde international cuisine, outdoor dining, Oligo restaurant offers classic Mediterranean breakfast recipes, Air Ibiza rooftop terrace with pool and panoramic views, Alabastro Lounge Bar with tapas / snack menu, and Wellness bar.