Sentences with phrase «with phospho»

Transgenic lines, from left to right, are paired sets from lines containing all components but lacking the TNT receptor -LRB-- TNT receptor); lacking the chimeric histidine kinase -LRB-- Trg - PhoR); lacking the protein for nuclear translocation / transcriptional activation -LRB-- PhoB - VP64); with the phospho - relay disrupted (PhoBD53A - VP64).

In addition, the presenter will provide guidance on experimental design for multiplexed antibody panels combining phenotypic markers with phospho - specific intracellular targets, and discuss the use of fix and perm kits.

Western blot analyses with phospho - specific antibodies suggested that stretching induces phosphorylation of ERK of the MAP kinase pathway, but did not induce phosphorylation of phosphatidylinositol 3 - kinase.

Not exact matches

Tim speculated that it might in fact work more like a «labeler,» tagging many diverse proteins with a «phospho - kiss of death.»

Membranes were incubated overnight at 4 °C with antibodies specific for phospho - Smad1 / 5/8 and Smad1 (Cell Signaling Technology), V5 (Invitrogen), or β - actin (Santa Cruz Biotechnology Inc.) in PBS containing 5 % nonfat milk and 0.5 % BSA.

A specific form of phospho protein phosphatase 2 regulates anaphase - promoting complex / cyclosome association with spindle poles.

In these plants, there is no significant difference in the GUS activity with or without the TNT ligand, indicating that the complete signal transduction system and phospho - relay through PhoB - VP64 is required for transcriptional activation.

To characterize activation of immune cells, measurement of intracellular signaling using phospho - specific antibodies combined with immune phenotyping using surface markers is ideal.

Cell lysates were subjected to western blotting and sequentially probed with Abs to phosphorylated p38 (phospho - p38), total p38 or actin.

Importantly, the phospho - ERK1 / 2 signal was completely abrogated upon lethal toxin treatment, which also correlated with loss of MEK1 and MEK2 (Fig 4A).

and affinity of antibody ACI - 5400 were characterized by a panel of methods: (i) measuring the selectivity for a specific phospho - Tau epitope known to be associated with tauopathy, (ii) performing a combination of peptide and protein binding assays, (iii) staining of brain sections from mouse preclinical tauopathy models and from human subjects representing six different tauopathies, and (iv) evaluating the selective binding to pathological epitopes on extracts from tauopathy brains in non-denaturing sandwich assays.

Furthermore, using a phospho - tyrosine [Y] 474 (in - house generated) and a phospho - serine [S] 176 (Cell Signaling, Inc., Danvers, MA) RIPK2 antibodies, we confirmed that active RIPK2 is recognized by these antibodies upon activation with MDP treatment in 293HEK fibroblast and HCT116 colon cancer epithelial cells (Fig. 3E, left panel) and detection of constitutively active RIPK2 in the colon cancer cell line SW480 (Fig. 3E, right panel).

(C) Measurement of Phospho - Erk levels in V+S + and V − S + fractions shows an enrichment of activated Erk with Venus positive ES cells.