Sentences with phrase «with reference sequences»

Night after night Rienhoff tediously compared his daughter's DNA sequence with reference sequences stored in several major genomic databases — Ensembl, Heidelberg, and the UCSC Genome Bioinformatics gene bank, among others.

Complete Genomics does not produce assemblies (evidence intervals) for regions of the genome where the mapped reads are highly consistent with the reference sequence.

Not exact matches

Incompatibility between the two software packages used caused some variants that the Ethiopian man shared with Europeans (whose DNA forms a large chunk of the human reference sequence) to be removed from the analysis.

Software lines them up based on overlapping stretches of code and comparisons with previously sequenced reference genomes.

We analyzed 7.2 terabases of metagenomic data from 243 Tara Oceans samples from 68 locations in epipelagic and mesopelagic waters across the globe to generate an ocean microbial reference gene catalog with > 40 million nonredundant, mostly novel sequences from viruses, prokaryotes, and picoeukaryotes.

«Humans vary in their DNA sequences, and what is taken as the «normal» DNA sequence for reference can not account for all these differences,» says Stuart Orkin, MD, of Dana - Farber Boston Children's Cancer and Blood Disorders Center and co-corresponding author on the study with Matthew Canver, an MD - PhD student at Harvard Medical School.

All 86 trisomy 21 cases, 82 reference controls, and 146 non-trisomy 21 cases were sequenced with the 2 - plex protocol (fig 1 ⇑).

Among the sequenced samples, we randomly chose 96 pregnancies with euploid male fetuses as the reference control set for z score calculation.

After mapping, a custom bash function was used to remove reads with more than three mismatches compared to the mitochondrial DNA reference sequence; the revised Cambridge Reference Sequence (rreference sequence; the revised Cambridge Reference Sequence (rCsequence; the revised Cambridge Reference Sequence (rReference Sequence (rCSequence (rCRS)[31].

Using simulations, we show the method is accurate across a variety of read lengths, with different length reference sequences, at different sample depths, and when samples contain reads originating from organisms absent from the reference.

Here, Jeong - Sun Seo and colleagues use a combination of recently developed genome sequencing and assembly technologies, including single - molecule real - time sequencing and next - generation mapping, to generate a higher - resolution reference genome with fewer gaps.

Pepper concludes, «The overall strategy for genotyping - by - sequencing, marker discovery, and annotation that we have provided in this study will be useful for researchers working with the many economically important allotetraploid species (such as the crop brassicas), but can be extended to any species, including those that do not currently have a reference genome.»

An additional 6 isolates with varying morphotypes retrieved from the UK HPA reference library will also be sequenced and compared for further identification of mutations that may lead to morphology differences.

This document provides an overview of Complete Genomics data and references to additional documentation, tools, and resources that will familiarize you with Complete Genomics data and maximize the value of your sequencing project.

Novel insertions of mobile elements into the sequenced genome are identified as clusters of reads that uniquely map to the reference genome with one arm and to ubiquitous sequence with the other arm.

This figure shows coverage profile plots for individual genomes in the reference genome set, along with the coverage profile of the sequenced genome of interest (represented by the thicker grey line).

As with other model organisms, the fully sequenced zebrafish genome has acted as an invaluable reference tool for scientists.

The combination of extensive introgression between rabbit subspecies together with the availability of a rabbit reference genome sequence provides ideal conditions to resolve the genetic basis and architecture of persistent reproductive barriers between hybridizing taxa in the early stages of divergence.

It represents a significant filling - in of gaps in a draft maize sequence announced a year and a half ago, but more importantly, comes with what amounts to a detailed reference manual, a set of comprehensive annotations.

This is a big leap forward with respect to the current gold standard of using one reference, 3D7 v3, which is the whole genome sequence of a single parasite.

De novo assembly of > 1 kb regions in the absence of a reference genome produced contiguous sequences (contigs) that aligned with highest similarity to XMRV genomes and not to related MLVs (data not shown).

The rationale behind this cutoff is that we argue 3 reads are the absolute minimum required to call a heterozygous variant - two reads with a non-reference base (to exclude sequence artifacts affecting only one read) and one with the reference base.

She emphasized several times the value of TCGA's GBM sequencing as a reference cancer genome, with high - quality mutations already profiled for mutations, gene expression, etc..

We identified rare variants with allele frequency up to 1 % in a reference panel of 433 European individuals from modern Finland, Spain, Italy, Netherlands and Denmark, for which genome - wide sequence data are available16, 17,18.

We additionally evaluated a collection of new de novo long - read haploid assemblies and conclude that although the new assemblies compare favorably to the reference with respect to continuity, error rate, and gene completeness, the reference still provides the best representation for complex genomic regions and coding sequences.

NCBI provides Gene, Online Mendelian Inheritance in Man, the Molecular Modeling Database (3D protein structures), dbSNP (a database of single - nucleotide polymorphisms), the Reference Sequence Collection, a map of the human genome, and a taxonomy browser, and coordinates with the National Cancer Institute to provide the Cancer Genome Anatomy Project.

References to older films of the genre, witty dialogue (including a monologue delivered by Carradine near the end that is probably the best thing Tarantino has ever written), extreme violence, a soundtrack filled with all kinds of extraordinary pop songs; even an entire backstory sequence completely done in Anime.

It makes for a pleasing metaphysical subtext to a film with spectacular action sequences, pointed references to the political economics of the class struggle, and a character in Benicio Del Toro whose nihilism carries with it a whiff of Zen philosophy at its purest.

As with any MacFarlane effort, where he once again teams with co-writers Alec Sulkin and Wellesley Wild, pop - culture references is the first port of call for joke - central, and the sequel is again over-loaded with them: the Planes, Trains and Automobiles and The Breakfast Club sequences are a hoot, but the Comic Con finale feels flat, more creative auto - pilot than uncharted waters.

Girls wear dance costumes, show legs and swing hips in dance sequence accompanying song with some sexual references.

The effects team took a research trip with the crew and went whitewater rafting, capturing video footage to use as reference for a big sequence where Arlo gets carried downriver.

In other words, almost everything in this film feels like a reference to another movie, but it's expertly assembled to look fabulous from start to finish, with some seriously striking sequences along the way.

«Rango» is a mélange of influences, but it's saying something that Verbinski could reference «Apocalypse Now» (complete with a banjo - plucking rendition of «Ride of the Valkyrie» by Hans Zimmer) and «Deliverance» (or maybe it's «Texas Chainsaw Massacre «-RRB- during a sequence that also features Johnny Depp, as a conflicted chameleon, flying through the air while wearing a dress.

Another interesting tidbit she offered up was a hint at what kind of action we could expect, referencing that it would be similar in ways to the free - running chase sequence that kicked off Casino Royale but with swords.

House elves are just as prominent in the U.S. wizarding world as they are in the UK, and I'd imagine if you freeze frame that opening sequence, the newspapers are loaded with more references.

«Penguin Pandemonium» demonstrates the progress of a single sequence, from mapping out shots to lighting reference pass to different stages of animation, with commentary from Waters, Green, and Hollander.

There is also a strong sense of humour with laugh - out - loud story sequences and many references to indie games in the stage backdrops.

It shows titles reading «Act One» and «Sequence One 1 - 15», with what appears to be an opening monologue underneath that includes references to the Nile, a fire, and the Four Horsemen.

With The Grand Budapest Hotel, Anderson seems to be not so concerned with history, but with the history of cinema; we can see references to Kubrick and F.W. Murnau, and the plot descends into an elaborate caper full of bizarre character studies, wondrous sequences (including a superb cat - and - mouse chase where Gustave and Zero zoom down a precarious mountain atop a toboggan in pursuit of Willem Dafoe on skis), and meticulously - designed, glamorous sets that are reminiscent of the traits of classical Hollywood films and murder - mysterWith The Grand Budapest Hotel, Anderson seems to be not so concerned with history, but with the history of cinema; we can see references to Kubrick and F.W. Murnau, and the plot descends into an elaborate caper full of bizarre character studies, wondrous sequences (including a superb cat - and - mouse chase where Gustave and Zero zoom down a precarious mountain atop a toboggan in pursuit of Willem Dafoe on skis), and meticulously - designed, glamorous sets that are reminiscent of the traits of classical Hollywood films and murder - mysterwith history, but with the history of cinema; we can see references to Kubrick and F.W. Murnau, and the plot descends into an elaborate caper full of bizarre character studies, wondrous sequences (including a superb cat - and - mouse chase where Gustave and Zero zoom down a precarious mountain atop a toboggan in pursuit of Willem Dafoe on skis), and meticulously - designed, glamorous sets that are reminiscent of the traits of classical Hollywood films and murder - mysterwith the history of cinema; we can see references to Kubrick and F.W. Murnau, and the plot descends into an elaborate caper full of bizarre character studies, wondrous sequences (including a superb cat - and - mouse chase where Gustave and Zero zoom down a precarious mountain atop a toboggan in pursuit of Willem Dafoe on skis), and meticulously - designed, glamorous sets that are reminiscent of the traits of classical Hollywood films and murder - mysteries.

speaking activity Le menu du jour / J'ai faim * — vocabulary writing practice Le verbe PRENDRE * La salle a manger café / À la carte - reading comprehension Commander au restaurant * - reading comprehension Au restaurant - listening activity with You tube reference Chez Aiello script / Sequencing worksheet *, a sheet for students on an IEP QR Code Scavenger Hunt — reading / writing activity Que commandent - ils?

The team of competent writers is working continuously in offering high - quality essay work to the student with a proper sequence of reference list and as per the need of a student.

When I heard it described as anime I incorrectly assumed this was in reference to the game's over-the-top style, but the game actually is laid out like television episodes, complete with interludes for non-existent commercial breaks, previews and credit sequences throughout.

Bryan Robertson: I've always understood, maybe over-simplistically, that the great abstract art of this century came about by a process of working through reality or some aspect of the physical world — the nude, landscape, the interior or still - life — in stages towards simplification, and then, like a sort of exorcism, a casting away of what Rothko called «crutches», venturing into some form of abstraction without any obvious references to the physical world, but maybe with some distilled, remembered vestiges of its appearance — like Mondrian's sequence of trees.

Though the individual drawings, which make up the film sequences, exist for only a brief second in real time, their digital record provided the artist with references for a set of 7 intaglio prints, collectively called The Music of Things, presented in a box set by Stoney Road Press.

Manglano - Ovalle scrupulously edits out all clear reference to this odd «kill your fathers» ritual, leaving the viewer with a dream - like sequence in which well - shod anonymous masses eternally exit and equally anonymous custodians endlessly move in to sweep up the crystalline debris of modernism.

With reference points ranging from Fang sculpture to Mississippi juke joints, Duchamp's urinal to jazz, he is a filmmaker with a unique understanding of how to cut and juxtapose a sequence to draw out maximum visceral effWith reference points ranging from Fang sculpture to Mississippi juke joints, Duchamp's urinal to jazz, he is a filmmaker with a unique understanding of how to cut and juxtapose a sequence to draw out maximum visceral effwith a unique understanding of how to cut and juxtapose a sequence to draw out maximum visceral effect.

Make a graph with the rises randomly spliced into the record, and see how many could pick the right sequence without reference to «the real record».

With recent announcements of plans for sequencing the genome from present - day polar bear (34) offering a necessary reference, future sequencing of all or a substantial fraction of the nuclear genome of this exceptionally well preserved Pleistocene polar bear specimen may be feasible.

Because the computer with which we conducted the steps for this tutorial (as we personally test and confirm all steps in all articles we write here at How - To Geek) would not cooperate with our capture tool during the time it was booted into Windows PE we recreated the sequence in a virtual machine expressly to create the screenshots for your reference.

Using the operating sequencing system procedures, status boards and other reference materials to communicate with Action Area Supervisors, Engineering Departments, Damage Control department, and the Supply department to coordinate system initialization, configuration changes, and casualty control restoration.