Cyclic adenosine monophosphate (cAMP)- dependent protein kinase, labeled
with fluorescein and
rhodamine on the catalytic and regulatory subunits, respectively, was injected into Aplysia sensory neurons either in culture or in intact cell clusters.
For double labeling, the cells were incubated
with primary antibodies against TuJ1 and L1 or GFAP, then biotinylated anti-mouse or rabbit (rat) IgG (Vector, Burlingame, CA, USA), and finally
with avidin -
rhodamine (Vector) and corresponding FITC - conjugated secondary antibodies (from different host species, Chemicon and Molecular Probes, Invitrogen).