Sentences with phrase «with ribozymes»

To date 10 HIV positive patients have been treated with ribozymes developed in the lab.

Not exact matches

By this directed evolution we were able to produce ribozymes that can catalyze the copying of relatively short strands of other RNAs, although they fall far short of being able to copy polymers with their own sequences into progeny RNAs.

The reaction is analogous to that catalyzed by a replicase with C5 acting as the primer, GpNs as the nucleoside triphosphates, and a sequence in the ribozyme providing a template.

Large protein complexes with RNA are found in the ribosome particles, which are in fact «ribozymes».

Using the atomic structures of the large ribosomal subunit fromHaloarcula marismortui and its complexes with two substrate analogs, we establish that the ribosome is a ribozyme and address the catalytic properties of its all - RNA active site.

The contribution made by individual functional groups of the G.U pair in the context of the Tetrahymena ribozyme was examined by replacement of the G.U pair with synthetic base pairs that maintain a wobble configuration, but that systematically alter functional groups in the major and minor grooves of the duplex.

The ribozyme active site also makes tertiary contacts with a tripod of 2» - hydroxyls on the minor groove surface of the splice site helix.

There are already some companies dedicated to bringing ribozymes from the laboratory to the commercial world, with potential applications as sensitive sensors of biowarfare germs or as medical diagnostic tests.

Szostak's work with synthetic aptamers and ribozymes has convinced him that RNA could have once dominated the world.

In total, the ribozyme could add on 14 nucleotides, with an accuracy of roughly 97 percent.

In 1993 David Bartel, then a graduate student with Szostak, produced a ribozyme that could join another piece of RNA to itself.

They tested it in a rat model of ADRP and found that after 2 to 3 months, eyes that had been injected with the virus - ribozyme combo contained 30 to 40 % more rod cells than eyes that received a dummy injection.

Because of the retroviral packaging signal, the ribozyme would be expected to colocalize with the lacZ - containing viral genomic RNA but not with the lacZ messenger RNA.

In some prokaryotes as well as in the organelles of some eukaryotes, splicing can be self - catalyzed by particular ribozymes with the help of magnesium ions, without the intervention of any protein machinery: the group II introns ribozymes are in fact able to self - regulate their own removal from the filament, thus promoting the maturation of messenger RNA.

After 24 rounds of this test tube evolution, in which the scientists successively upped the requirements for what a RNAP ribozyme had to do to be successful, they wound up with one called 24 - 3 polymerase.

So Joyce and his postdoctoral assistant David Horning attempted to come up with a more versatile RNAP ribozyme, using a well - known technique known as in vitro evolution.

Ribozymes are RNA molecules with enzymatic activities.

I managed to get a great many articles in evolutionary biology (e.g., retroelements, ribozymes, phages) that way with the Proceedings of the National Academy of Sciences.