Sentences with phrase «with subcellular»

With the subcellular location restricting a protein's possible function, this method should be a useful tool for the systematic analysis of genome data and is available via a server on the world wide web.

The careful sample preparation and fluorescence light microscopy under close - to - natural conditions allow us to image large volume elements in plants with subcellular resolution.

The article gives an overview on techniques that are used to locate proteins within cells and Emma Lundberg shares her experiences from her work with the Subcellular Protein Atlas within the Human Protein Atlas, HPA.

«We expect that our new method will become an attractive tool to study energy requirements of living systems with subcellular resolution,» says Min, «especially in brain and malignant tumors that are in high demand of energy.»

Not exact matches

«The cytoskeleton system is comprised of a host of interacting subcellular structures and proteins, and our technique will enable research on the interactions between these different targets with unprecedented number of color channels and spatial resolution,» he said.

The authors of this study combined live cell imaging with electron microscopy to observe Trichoplax feeding behavior at scales ranging from the whole animal to subcellular.

With its ability to monitor cell function, PET is the archetype of the new tools that can monitor our bodies at the cellular or even subcellular scale, says Bruce Hillman of the American College of Radiology Image Metrix.

Miniscule carriers, the motor proteins, slide along the microtubules with great volumes such as chromosomes, vesicles and other subcellular components — like mitochondria — latched onto them.

Korenberg's team is developing a 3D coordinate system to align various types of neuroimaging data in the macaque brain, from whole - brain MRI connectivity to single - cell confocal data and, for some areas, subcellular resolution with electron microscopy.

Another technique pinpoints the locations of proteins within their subcellular context by coupling super-resolution imaging with electron microscopy.

A major restructuring of the knowledge portal has been done with the release of four separate sub-atlases; Normal tissue atlas, Subcellular atlas, Cell line atlas and Cancer atlas, and RNA transcript data has been added for a majority of the tissues in the normal tissue atlas and the cells in the cell line atlas.

The ability to label different subcellular locations with the green fluorescent protein (GFP) has made it possible to visualize intracellular activities in living cells.

By combining local light - induced heat sources with sensitive nanoscale thermometry, it may also be possible to engineer biological processes at the subcellular level.

This extreme morphology raises many cellular challenges: in particular, the delay required to transport organelles and cargo from the soma to distant parts of the axon can reach several hours and is incompatible with the rapid adaptation of subcellular homeostasis.

The researchers also examined the subcellular localization of ApoE proteins in human neuroblastoma cells transfected with either ApoE3 or ApoE4, detecting the molecules in both the cytoplasm and the nucleus.

We invite original innovative research on the rational engineering of plant systems at all levels, including proteins, protein complexes, sensors, metabolic and signalling pathways, microcompartments such as carboxysomes, subcellular compartments, cell types or tissues, as well as the engineering of novel plant hybrid species and the interactions of plants with viruses, bacteria, and other organisms.

The examination of the amyloid pathology with super-resolution microscopy permitted us to further reveal a clear separation of the subcellular location of Aβ - immunoreactivity from that of APP / CTFs.

Deputy Editor Steven Carr of The Broad Institute will talk about new approaches for subcellular localization of proteins and their interaction partners with high spatial resolution.

He works with a group on the Cell Atlas, a high resolution subcellular atlas showing the spatial distribution of proteins within cells.

«After releasing the sacrificial layer, we were able to harvest substrate-less small - sized probes with a multilayer layout that markedly reduces their cross-sectional area to the subcellular range,» explains Wei.

In the first step, stainings in different cell lines with the same antibody are reviewed and the results are compared with available protein / gene characterization data for subcellular location.

In addition, we prepared a large number of tools to manipulate signalling networks and are able to locally activate the important messenger such as PIP3 and DAG with a light flash in subcellular resolution in living cells (Mentel et al. 2011; Nadler et al. 2013, Nadler et al. submitted).

We use modern microscopy techniques to examine how chloroplasts and mitochondria interact with each other and other subcellular structures.

Interferon regulatory factor subcellular localization is determined by a bipartite nuclear localization signal in the DNA - binding domain and interaction with cytoplasmic retention factors

Questions remain concerning why neurons that apparently express similar innexins (the different motor neuron classes) rarely form gap junctions with one another, if gap junctions are subject to constant formation and reformation in the nervous system, and why there appears to be a consistency in subcellular localization (soma versus process) of certain gap junctions.

The cellular localization of the polymers was confirmed by subcellular fractionation of the cells, followed by digestion with endoglycosidase H (Figure 2C and Supplemental Figure 3C), an enzyme that removes N - linked glycans still in the high mannose ER form but does not affect oligosaccharide chains after the addition of sialic acid in the Golgi apparatus.

METHODS: We treated 3T3 - L1 adipocytes with 2.5 mmol / l R (+) alpha - lipoic acid for 2 to 60 min, followed by assays of: 2 - deoxyglucose uptake; glucose transporter 1 and 4 (GLUT1 and GLUT4) subcellular localization; tyrosine phosphorylation of the insulin receptor or of the insulin receptor substrate - 1 in cell lysates; association of phosphatidylinositol 3 - kinase activity with immunoprecipitates of proteins containing phosphotyrosine or of insulin receptor substrate - 1 using a in vitro kinase assay; association of the p85 subunit of phosphatidylinositol 3 - kinase with phosphotyrosine proteins or with insulin receptor substrate - 1; and in vitro activity of immunoprecipitated Akt1.

Pathogenesis of FISS Several sophisticated studies have addressed cellular and subcellular changes associated with FISS.