Sentences with phrase «with supernatant»

Red histograms on each graph show target cells incubated with supernatant from unmodified HB1.F3 NSCs as a negative control.

Flow cytometric analysis of BT474, MCF7 / HER2, or MCF7 target cells after incubation with supernatant from HB1.F3.H2IgG, HB1.F3.Adeno - H2IgG, or HB1.F3.Lenti - H2IgG (C), followed by incubation with FITC - conjugated anti-human IgG (blue histograms).

HeLa cells were infected with supernatant from the murine macrophage cell line RAW264.7 (ATCC, Manassas, VI) known to express polytropic and ecotropic MuLV (PMLV and EMLV, respectively).

Cultured pericytes abundantly expressed calpain1 (Fig. 2d) and showed a significant induction of calpain1 activity after stimulation with supernatants of Ang2 overexpressing HUVEC (Fig. 2e).

Stimulation of pericytes with supernatants from either Ang2 overexpressing HUVEC or supernatants from control HUVEC resulted in significantly increased pericyte migration following Ang2 stimulation (Fig. 2c, Supplementary Fig. 4c, d).

Not exact matches

By securing a pellet (with neodymium magnets) on the sides of the tube walls rather than at the bottom, it allows complete removal of the supernatant without touching the pellet.

With the current designs of magnetic stands, these purifications can be problematic, often leaving carryover contaminants when the pellet is disturbed or when the supernatant is not completely removed.

After achieving 80 — 90 % confluency, cell culture media were changed to maintenance media with 2 % FBS and were inoculated with 200 µL of clinical sample or 100 µL of passaged viral supernatant.

The supernatants were discarded, and the tissue was resuspended in DMEM supplemented with 10 % fetal bovine serum, 2 - mm l - glutamine, sodium pyruvate, nonessential amino acids, and a vitamin solution (Life Technologies, Inc., Rockville, MD).

The group has over 10 years» experience with this methodology, with specific expertise in cytokine / chemokine detection in tissue culture supernatants and plasma samples with panels containing up to 42 analytes.

Maternal plasma previously acidified 1 ∶ 1 with ice - cold 10 % metaphosphoric acid (MPA) was centrifuged and the supernatant stored at − 70 °C.

Loaded gesicles can then be collected from the supernatant and added to target cells for efficient editing with no footprint.

Briefly, 100 ml of culture supernatants were mixed with AVL - Carrier RNA buffer.

Spleen CD4 + T cells isolated from BMT recipients on day 21 were treated with vehicle solution or VPA for 24 h, the supernatants were collected, and ELISA was performed to determine the level of IFN - γ (C) and IL - 17A (D).

Forty - eight hours after transfection, the media were centrifuged and the supernatant was diluted with culture medium to 100 %, 50 % and 25 % the original concentrations.

Assessment of hASC - CM composition found high expression of various human growth factors (IL ‐ 6, 8, 12, eotaxin, IP10, MCP ‐ 1, VEGF, and TIMP ‐ 1) in the supernatant following the co-culture of hASCs with islet cells, while IP10, eotaxin, VEGF, and TIMP ‐ 1 became increased with time during islet co ‐ culture, suggesting the presence of paracrine cross ‐ talk between islets and hASCs.

With current designs of magnetic stands, these purifications can be problematic, often with carry over contaminants when the pellet is disturbed or when the supernatant is not completely remoWith current designs of magnetic stands, these purifications can be problematic, often with carry over contaminants when the pellet is disturbed or when the supernatant is not completely remowith carry over contaminants when the pellet is disturbed or when the supernatant is not completely removed.

After blocking for 1 hour with 10 % normal goat serum (NGS), the slides were incubated with 200 μl primary antibody, either a polyclonal rabbit anti-Borrelia (Accurate chemicals, Westbury, NY) at 1:200 or the anti-OspA monoclonal, CB10, with a 1:30 dilution of hybridoma supernatant for 1 hour at room temperature (RT).

The results revealed that the supernatant of 72 - hour ATRA - treated NB4 cells was sufficient to induce the tyrosine phosphorylation of STAT2 and the endogenous RIG - G level in U3A cells, in comparison with the relative consistent level of total STAT2 (Fig. 3B).

The cell culture supernatant was collected and concentrated if necessary, and then 100 μL aliquots were used for detection with ELISA kit (PBL Biomedical Laboratories) following the protocol of the manufacturer.

Cell supernatants were discarded and cell pellets were lysed of RBCs and then used for staining with antibodies for flow cytometry analysis.

(C) RIPK2 in HCT116 cells were IP with an anti-RIPK2 antibody (A) and the supernatant after IP in (A) was IP in (B) with the same RIPK2 antibody, and in vitro kinase (IVK) assay was carried out on both samples.

After 24 hours (on day 1) the mixed viral supernatant was removed, the cells were washed twice with PBS and then cultured in fresh MEF medium.

Cells were incubated with GCTM2 hybridoma supernatant (mouse IgM) for 30 min at room temperature following by three washes with 100 mM Tris - HCl, pH 8.5.

To confirm specificity, supernatant from transfected or transduced HB1.F3 cells was incubated with MCF7, MCF7 / HER2, or BT474, a human breast cancer cell line that endogenously overexpresses HER2.

On the following day (considered day 0) the concentrated retroviral supernatants were thawed and mixed at a 20x OCT4, 10x SOX2, 10x KLF4, 10x cMYC ratio, supplemented with fresh MEF media up to 2 ml volume (per well) and 8 ng / ml polyprene and then exposed to the HUF1 cells at 37 °C and 5 % CO2.

On day 3 the mixed viral supernatant was again removed, the cells were washed twice with PBS and then cultured in fresh MEF medium.

The supernatant was mixed with 300 µl of Ni - NTA agarose (Qiagen), and the proteins were allowed to bind for 3 hours, followed by washing with 20 ml of resuspension buffer, and eluted with 500 µl of elution buffer (20 mM NaHPO4, 300 mM NaCl, 10 % glycerol, 150 mM Imidazole, pH 7.8).