Sentences with phrase «with tg»

Hello William, I am just experiencing a similar situation with TG.

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Tg - hIAPP mice inoculated with Tg pancreas homogenate containing IAPP aggregates (hereafter termed the Tg / Tg group) exhibited punctate staining with anti-IAPP antibody (Fig. 3 A) and ThS (Fig. 3 B), starting at ∼ 8 wk of age.

These models include in vitro experiments with purified recombinant or synthetic proteins, cell culture experiments, and infectivity studies with Tg mice expressing the appropriate form of the protein.

The picture corresponds to a representative image of one animal treated with Tg - hIAPP pancreas homogenate and sacrificed at 20 wk old.

No significant difference in body weight was observed in the groups treated with diabetic pancreas homogenate, containing IAPP aggregates or synthetic IAPP aggregates, prepared in vitro, compared with Tg - hIAPP mice injected with buffer (Fig. 8 A).

Indeed, the number of β cells / mm2 of pancreas in the Tg / Tg group was on average > 60 % lower in comparison with Tg / WT mice (Fig. 4 E).

Fig. 3 D shows one representative image of that costaining for an animal of the group treated with Tg - hIAPP pancreas homogenate and sacrificed at 20 wk old, depicting the ThS signal in association with β cells.

Fasting blood glucose level became significantly higher in animals injected with Tg - hIAPP pancreas homogenate containing IAPP aggregates, as early as 8 wk compared with mice treated with WT pancreas extracts (Fig. 4 A).

(C) To evaluate impairment in glucose tolerance, the i.p. glucose tolerance test was performed in 16 - wk - old, Tg - hIAPP mice inoculated with pancreas extracts from old Tg - hIAPP (red line) as compared with Tg - hIAPP mice inoculated with PBS (black line).

To find out, the «Mad Money» host sat down with TG Therapeutics CEO Michael Weiss.

The news kicks things off with TGS 2009 — the ups and downs from this years «best» conference.

With TGS approaching, however, rumors are picking up once again that Resident Evil 6 will at least be teased at the show.

With TGS 2015 coming soon, it appears the game has been leaked to be shown at the Japanese games convention for the very first time.

I took a spin with the TGS demo on an iPad to see how the game fared on a tablet.

19th September 2012 - With TGS 2012 kicking off today Sony has started with sort of a bang by announcing a new PS3 Slim model which is now, wait for it — simmer!

It's been a busy week, especially with the TGS that started recently.

Not exact matches

TG: When I was in Pebble Beach, I had a nice chat with Canucks president and general manager Mike Gillis.

Beyond the cardiovascular risk associated with LDL - C, genetic, epidemiologic, clinical and real - world data suggest that patients with elevated triglycerides (TG)(fats in the blood), and TG - rich lipoproteins, are at increased risk for cardiovascular disease.

Honestly, do people like TG not realize the world is full of other religions, with people who have also had spiritual experiences within the framework of their own belief system?

TG - Studio worked with him to redesign the three - story property, originally an old barn before being converted into an office space.

References: Dunn J, Cheng H, O'Connor TG & Bridges L (2004) «Children's perspectives on their relationships with their nonresident fathers: influences, outcomes and implications» Journal of Child Psychology and Psychiatry and Allied Disciplines 45 (3): 553 - 566

I now have 4 children TG and I see with each baby how the situation is slowly improving, especially among mothers» desire to breastfeed, which in turn drags the reluctant medical establishment into the modern era.

Increased LDL - C, HDL - C, and possibly TG levels were associated with a lower risk of diabetes.

An analysis using genetics finds that increased low - density lipoprotein cholesterol (LDL - C), high - density lipoprotein cholesterol (HDL - C), and possibly triglyceride (TG) levels are associated with a lower risk of diabetes, and increased LDL - C and TG levels are associated with an increased risk of coronary artery disease, according to a study published online by JAMA Cardiology.

Michael V. Holmes, M.D., Ph.D., of the University of Oxford, England, and colleagues examined the associations of LDL - C, HDL - C, and TG levels with CAD and diabetes through mendelian randomization (MR) using conventional MR and making use of newer approaches using genetics.

«While MR studies have been successful in solving the causal relevance of major lipids in coronary heart disease (LDL - C and TG, yes, and HDL - C, no), it seems that other approaches are required to further evaluate the causal relevance of each of these lipid fractions in association with T2D.

The six benchmarks are: 1) HDR brachytherapy procedures are supported with the appropriate team as described in the report of the AAPM TG 59 and the American College of Radiology HDR Brachytherapy Practice Standard; 2) commissioning of the treatment unit, treatment planning system and each new source is performed by a qualified medical physicist and verified through a QA process; 3) assay of the HDR brachytherapy unit source is performed using a well - type ionization chamber with a calibration traceable to the National Institute of Standards and Technology, and this assay is performed or confirmed for each source change.

Breed MMTV - PyMT + / tg; Postn + / − or MMTV - PyMT + / tg; Postn − / − male mice with Postn + / + and Postn − / − female mice to obtain MMTV - PyMT + / tg; Postn + / + control and MMTV - PyMT + / tg; Postn − / − experimental female mice, respectively.

Image analysis studies of the IAPP staining (Fig. 3 A) show that both the percentage of islets containing IAPP aggregates (Fig. 3 E) and the load of IAPP deposits (Fig. 3 F) in the Tg / Tg group of mice progressively increased with age.

Islets isolated from 3 wk - old Tg - hIAPP mice were treated with different concentrations of synthetic IAPP - aggregated seeds for 10 d.

(A) Isolated islets from 3 - wk - old, female, Tg - hIAPP mice were cultured in presence of different concentrations of islet extracts (IE) from old Tg - hIAPP mice, with overt diabetic pathology and age - matched WT mice.

To rule out that the staining was coming from the aggregates present in the inoculum, cultured islets from WT animals, which do not express hIAPP, were incubated with the same amount of old Tg islet homogenate.

Representative images of the islets from Tg - hIAPP mice treated with synthetic IAPP aggregates, the same quantity of nonaggregated protein, or the controls treated with PBS.

(E and F) Black bars represent the animals inoculated with old Tg - hIAPP pancreas homogenate, and white bars correspond to the controls injected with WT pancreas.

b. For generation of the MMTV - PyMT + / tg; Postn − / − experimental female mice, male MMTV - PyMT + / tg; Postn − / − male mice will be crossed with Postn − / − female mice.

Immediately after counting macrometastasis, use the first six lungs identified from MMTV - PyMT + / tg; Postn + / + female mice that are positive with metastatic disease for further analysis (Protocol 2).

All in vivo experiments, including controls and inocula, were performed with male Tg mice.

Conversely, Tg - hIAPP mice inoculated with WT pancreas homogenate (hereafter referred to as the Tg / WT group) only began to show small IAPP aggregates at around 20 wk of age (Fig. 3, A and B), in a manner similar to untreated Tg mice.

(A) Isolated islets from 3 - wk - old, female, Tg - hIAPP mice were cultured in presence of different concentrations of IAPP aggregates prepared in vitro from synthetic IAPP, as well as controls treated with other amyloidogenic proteins, including the Alzheimer's disease — associated protein Tau (the K18 fragment) and the bacterial amyloid Mcc.

In our studies, we observed hyperglycemia and impaired glucose tolerance in the Tg - hIAPP mice treated with IAPP aggregates, which occurred concomitant to a significant loss of β cells (> 60 %).

Groups of male Tg - hIAPP mice were injected i.p. at 3 wk of age with 10 % pancreas homogenate from either 12 - mo - old, male, IAPP Tg mice bearing substantial islet amyloid aggregates (as shown in Fig. 1) or from age - matched, male, WT mice not expressing hIAPP.

The load of IAPP aggregates in islets treated with old Tg - hIAPP islet homogenate was significantly greater than that of the control groups, including untreated human islets and those exposed to the same concentration of WT islet extracts lacking IAPP aggregates (Fig. 2 D).

These abnormalities were not observed during the time of our experiments in Tg - hIAPP untreated mice or mice injected with pancreas homogenate without IAPP aggregates.

The results showed that Tg - hIAPP injected with pancreatic homogenate containing IAPP aggregates (the Tg / Tg group) exhibited an altered islet morphology characterized by the appearance of α - and δ - cells in the center of the islets, compared with the classical pattern in mice (observed in Tg / WT), in which these cells are mostly confined to the periphery of the islets (Brissova et al., 2005).

Our findings are in agreement with a recently published study by Oskarsson et al. (2015) in which Tg mice injected through the tail vein with in vitro — generated aggregates from synthetic peptides containing the sequence of IAPP developed a higher percentage of IAPP aggregates in the pancreas than did untreated controls when subjected to a high - fat diet.

As a control, a group of age - and gender - matched Tg - hIAPP mice were i.p inoculated with buffer (PBS).

Groups of male, IAPP, Tg mice were injected i.p. at 4 wk of age with 10 % pancreas homogenate from male, diabetic, IAPP Tg mice or 50 µM IAPP aggregates prepared from synthetic source.