This sensor was used to demonstrate that biomolecules can be successfully sealed
within microfluidic devices.
«This means that the system will open up new options for biosensing particles
within microfluidic devices.»
As it can take weeks to grow human cells into intact differentiated and functional tissues within Organ Chips, such as those that mimic the lung and intestine, and researchers seek to understand how drugs, toxins or other perturbations alter tissue structure and function, the team at the Wyss Institute for Biologically Inspired Engineering led by Donald Ingber has been searching for ways to non-invasively monitor the health and maturity of cells cultured
within these microfluidic devices over extended times.
With this method, they created a three - dimensional array of permeable carbon nanotubes
within a microfluidic device, through which fluid can flow.
To study this barrier and determine why a lack of blood flow causes it to leak, the researchers built a blood - vessel - on - a-chip model consisting of a channel lined with a layer of human endothelial cells surrounded by extracellular matrix
within a microfluidic device, which allowed them to easily simulate and control the flow of blood through a vessel and evaluate the cells» responses.
The primary epithelial cells are expanded as 3D organoids, dissociated, and cultured on a porous membrane
within a microfluidic device with human intestinal microvascular endothelium cultured in a parallel microchannel under flow and cyclic deformation.
Not exact matches
The
device continuously monitors conditions
within the
microfluidic chip, including oxygen levels, temperature, and pH, to ensure the optimum environment for cell growth.
Measuring two fundamental properties of surface chemical reactions on the same
device means that researchers can be far more confident that biomolecules have been successfully encapsulated
within the
microfluidic platform.
«These mechanical vibrations can, in turn, «talk» to liquids
within the hollow
device and provide optical readout of the mechanical properties,» said Bahl, who is first author of the paper, «Brillouin cavity optomechanics with
microfluidic devices,» published this week in Nature Communications.