Not exact matches
Both unmodified and genetically modified groups were found to have
cells that migrated and survived in two distinct locations: (i) as a separate, nearly continuous, subretinal layer lying between the host RPE and
photoreceptors, and (ii) as individual
cells distributed throughout the neurosensory retina, especially
within the inner retinal layers (Figure 5A).
Importantly, akin to HESC - RPE in vivo,
cells at the outside edge of the iPS - RPE
cell bolus could phagocytose
photoreceptor outer segments from the RCS rat, as indicated by the presence of rhodopsin - positive
photoreceptor material
within the cellular membrane of iPS - RPE labelled with HSM (Fig. 5E).
Phagocytosis of
photoreceptor outer segments was defined by the inclusion of rhodopsin - labelled material
within the TRA -1-85-bordered intracellular compartment of grafted human iPS - RPE
cells.
Within this broad topic we are particularly interested in characterising (i) the molecular mechanism by which these
photoreceptors mediate light - dependent entrainment of the circadian clock, (ii) the components mediating, in a light - quality - dependent fashion, nucleocytoplasmic partitioning of phytochromes and UVR8, (iii) how phosphorylation and sumoylation of these photorecepors and other signalling components modulate red / far - red and UVB - induced signalling, and (iv) to what extent intercellular and
cell - autonomous events contribute to phytochrome and UVR8 regulated photomorphogenesis.
However, the evidence from the past decade or so has revealed a third class of
photoreceptor within the eye; intrinsically photosensitive retinal ganglion
cells (ipRGCs), which express their own distinct opsin — melanopsin [6,7].
To further examine the morphology of
cells and the localization of protein expression
within the retina, immunohistochemical staining of both paraffin and OCT retinal sections was performed with the following antibodies (Table S1): human cone arrestin (for cone
photoreceptors), rhodopsin (for rod
photoreceptors), RPE65 (for the retinal pigment epithelium, RPE), glial fibrillary acidic protein (GFAP, for astrocytes and Müller
cells), glutamine synthetase (for Müller
cells) and G0alpha (for ON bipolar
cells).