Sentences with phrase «without immunoaffinity»
CSF proteins (from the immunoaffinity depletion processed pooled samples and the individual samples without immunoaffinity depletion processing) were digested with trypsin and cleaned up with SPE C18 columns as previously described [10].
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Individual CSF samples from 14 of the 43 CFS subjects (aged 33 — 48 years with a median age of 43 years, 7 female and 7 male) were also used in direct LC - MS analysis (i.e., no MS / MS was performed) without immunoaffinity depletion.
Individual CSF samples from another set of 10 healthy volunteers, age 37 — 44 years (median = 40) and 5 women and 5 men, were analyzed by LC - MS analysis without immunoaffinity depletion.