Sentences with phrase «xmap cytometric»

Although conventional flow cytometry is limited to measuring particles down to approximately 300 nm — 500 nm, a relatively new flow - cytometric method — called «imaging flow cytometry» — allows for the analysis of EVs smaller than 300 nm.

Using the Single - Cell Automatic Analysis and Isolation System developed in 2013, this group isolated single OSNs responding to specific odorants in a time - lapse single - cell - array cytometric manner and identified the OR gene through single cell polymerase chain reaction (PCR).

(B, C) Flow cytometric analysis of HIV - 1 gene expression in (B) mock infected or (C) latently infected CD4 + T cells under non-polarizing conditions, either at the basal state or after reactivation with antibodies against CD3 and CD28.

In this series of seminars, we will discuss the necessary tools to design, run, and analyze a multi-parameter flow cytometric experiment.

UNISI provides expertise in the measurement of cell signalling proteins, such as cytokines, chemokines and inflammatory biomarkers in multiple samples (including serum, plasma and tissue culture supernatants), using a multiplex suspension array system or a flow cytometric bead assay.

Flow cytometric analysis of the cells following anti-CD8 antibody and complement treatment indicated that < 2 % were CD8 +.

Flow cytometric analysis revealed that anti-Thy 1.2 antibody plus complement depleted > 97 % of the Thy 1.2 + cells (data not shown).

Flow cytometric analysis was performed using BDLSR Fortessa.

Subsequent single - cell flow cytometric assays linked this shorter G1 phase to the more rapid loading of minichromosome maintenance (MCM) helicase complexes when compared to differentiated cells with longer G1 phases (neural progenitor cells and retinal pigmented epithelial cells).

The facility collaborates with various research projects using flow cytometric techniques to isolate and purify cells and chromosomes for structural and functional studies, sequencing, microarrays as well as fluorescence insitu hybridisation (FISH) experiment.

Flow cytometric analysis of rabbit anti-CD3 (SP7) antibody ab16669 (1/100) in Jurkats cells (green) compare to negative control of rabbit IgG (blue).

The instructors will focus on a broad spectrum of flow cytometric topics, discuss examples from various research applications, provide technical protocols for flow cytometric sample preparation, as well as data analysis, trouble shooting and experimental design.

The facility supports a range of flow cytometric techniques and currently has 9 cytometers located at F2 - 33, F2 - 34, F2 - 35 and F2 - 36.

Additionally, an aliquot of 4 × 106 splenocytes was stained for Foxp3 expression using methods and reagents, provided by the manufacturer (eBioscience), prior to flow cytometric analyses.

To assess granzyme B production, CFSE - labeled cells were stimulated for 24 or 72 h, surface - stained as described above, permeabilized using a Cytofix / Cytoperm kit (BD Pharmingen), and then stained with flurochrome - labeled granzyme B Ab (GB11; Life Technologies) at 4 °C for 30 min prior to flow cytometric analyses.

Annexin V staining and flow cytometric analysis were performed according to the manufacturer's instructions (ebioscience, # 88-8005-74).

EdU incorporation and flow cytometric analysis were performed according to the manufacturer's instructions (Life Technologies, #C10634).

Join us for a discussion of a broad range of novel functional reagents and demonstration of their use in cell - based immunotherapy models using powerful cytometric instrumentation.

Sections from all cases were subjected to stain with haematoxylin and eosin (H&E) for histopathological examination and immunohistochemical technique for Cyclin D1 in addition using flow cytometric technique to perform cell cycle analysis.

Technical support is also provided for analyses of flow and imaging cytometry data for publication, presentation, and inclusion in grant applications, management of cytometric data (storage, archiving, and retrieval), and management of a site license for low - cost analysis software.

Variation of flow cytometric DNA measurement in 1,485 primary breast carcinomas according to guidelines for DNA histogram interpretation.

Sensitive and viable identification of antigen - specific CD8 + T cells by a flow cytometric assay for degranulation.

Flow cytometric technique provides a method for assessing the cells nuclear DNA content and proliferation rate by using the value of S - phase fraction (SPF)[12].

The homogenized fresh breast tumor samples have been represented for flow cytometric analysis using FACS calibur flow cytometer (Becton Dickinson, Sunnyvale, CA, USA) equipped with a compact air cooked low power 15 mwat argon ion laser beam (488nm), data analysis was conducted using DNA analysis program MODFIT (Topsham, USA).

Assisted / Supervised use The facility's staff will assist researchers in using FRACTAL strumentation, and will provide information for the preparation of samples for cytometric analysis.

The amount and intensity of the labeled beads were determined using a Luminex xMAP cytometric scanner (BioRad).

Flow cytometric analysis of asialoglycoprotein receptor (ASGPR) levels on the surface of hepatocytes, which were obtained from the liver specimens of patients that received hepatectomy, were used as predictors of liver dysfunction after majo...

When absolute purity is not necessary, as is often the case with in vitro stimulation of T cells, magnetic cell separation can deliver highly enriched cells faster, with significant cost - savings, and without exposure to harsh separation protocols like flow cytometric sorting, or chemical gradients.

Flow cytometric analysis of BT474, MCF7 / HER2, or MCF7 target cells after incubation with supernatant from HB1.F3.H2IgG, HB1.F3.Adeno - H2IgG, or HB1.F3.Lenti - H2IgG (C), followed by incubation with FITC - conjugated anti-human IgG (blue histograms).

UNISI will provide expertise in the measurement of cell signalling proteins, such as cytokines, chemokines and inflammatory biomarkers in multiple samples (including serum, plasma and tissue culture supernatants), using a multiplex suspension array system or a Cytometric bead assay.

A flow cytometric method to measure prokaryotic records in ice cores: an example from the West Antarctic Ice Sheet Divide drilling site Journal of Glaciology, in press, p. 1 - 19.

Since our flow cytometric data had shown that the HPCs poorly expressed MHC antigens [17], we wondered whether they stimulate allogenic MRL splenocytes (H2k).

These may include but are not limited to vaccine specific and advanced multi-label flow cytometric analyses.

(A, B) Mixed (1:1) CD11cDTR (CD45.1 +) and Ebi2 - / - or Ebi2 + / + (CD45.1 ʱCD45.2 +) BM chimeric mice were immunized with SRBCs and flow cytometric analysis of B cell responses performed five days later.

Eight weeks postinjection, the engraftment was confirmed by flow cytometric staining of peripheral blood cells for human CD45 + / CD33 + cells, followed by randomization into study groups and treatment with a single bolus IV injection of IMGN632 or the control ADC.

Intestinal permeability was assessed by Ussing chamber; epithelial cell (EC) ultra-structure by electron microscopy; RNA expression of genes coding for junctional proteins by Q - real - time PCR; immune response by in - vitro antigen - specific T - cell proliferation and cytokine analysis by cytometric bead array; intestinal microbiota by fluorescence in situ hybridization and analysis of systemic antibodies against intestinal microbiota by surface staining of live bacteria with serum followed by FACS analysis.