Sentences with phrase «xenograft cells»

We confirmed that the protein level of APC was elevated and the expression of miR - 150 was decreased in the human breast cancer xenograft cells transfected with the anti-miR-142-3p lentivirus (Figure 5E and Figure 7B).

1) The results of the inhibition of miR -142-3p in the human breast cancer xenograft cells and the Wnt signaling pathway inhibition in human breast cancer cells have confirmed that our finding that miR - 142 targets APC, activates the Wnt signaling pathway and upregulates miR - 150 is applicable to human breast cancers.

We further confirmed that the protein level of APC was elevated in the human breast cancer xenograft cells transfected with the anti-miR-142-3p lentivirus (Figure 7B).

To mimic the in vitro hypoxic response in vivo, we developed a xenograft model based on the hypothesis that with increasing xenograft sizes, there will be a parallel increase in hypoxia due to rapid cell proliferation and restricted blood supply to the xenograft cells.

Not exact matches

A decade ago, he replicated the entire human leukemia disease process by introducing oncogenes into normal human blood cells, transplanting them into xenografts (special immune - deficient mice that accept human grafts) and watching leukemia develop — a motherlode discovery that has guided leukemia research ever since.

Through these effects, the PERY peptide reduced the proliferation of several (but not all) cancer cell lines in culture and inhibited the growth of a human breast cancer xenograft in mice.

To overcome these problems, Min and his team developed a new modality to visualize glucose uptake activity inside single cells based on stimulated Raman scattering (SRS) imaging, and demonstrated its use in live cancer cells, tumor xenograft tissues, primary neurons and mouse brain tissues.

Terbinafine, a U.S. Food and Drug Administration — approved antifungal drug targeting SQLE, markedly inhibited SQLE - induced NAFLD - HCC cell growth in NAFLD - HCC and HCC cells and attenuated tumor development in xenograft models and in Sqle transgenic mice.

EZH2 inhibition delayed tumor onset in KDM6A - null cells and caused regression of KDM6A - null bladder tumors in both patient - derived and cell line xenograft models.

We created a mouse xenograft model in which SiHa cervical cancer cells were injected into mice subcutaneously, and the resultant tumors were treated with PAL three times a week for 6 weeks.

We thank the Penn Genome Frontiers Institute and the University of Pennsylvania's Stem Cell and Xenograft Core and DNA Sequencing Facility for 454 sequencing.

«There is some talk of going into 3 - D cell models rather than xenografts, because it is so difficult and challenging to grow [the transplanted tumors],» says Manish Kohli, the Mayo Clinic oncologist leading the PROMOTE study.

Moreover, following transformation with H - Ras, these cells initiated tumor xenografts in immunocompromised mice with higher efficiency than their epithelial counterparts [5].

NCI's efforts to develop new laboratory models of human cancer includes vastly increasing the number of human cancer cell lines (grown as two - dimensional and three - dimensional cultures) and patient - derived tumor xenografts.

Due to the high efficiency of establishing organoid models from different tissues and diseases, such as cancer, organoid technology allows the generation of large living biobanks of tumor organoids that are amenable for middle - throughput drug screens and may allow personalized therapy design, as a complement to cell line and xenograft - based drug studies (7,19).

Since starting her new role as an investigator in the In Vivo Pharmacology group at Novartis Oncology, Bhang has been working on using the ClonTracer library to monitor clonal dynamics following drug treatment in cell line and primary tumor xenograft models.

Using a zebrafish embryo xenograft model we also demonstrated that anti-CD90 monoclonal antibodies decreased the viability and metastatic potential of insulinoma cells, suggesting that anti-CD90 monoclonals form a potential novel adjutant therapeutic modality.

Zebrafish larva implanted with cancer patient's tumor (red) RITA FIOR / CHAMPALIMAUD CENTRE FOR THE UNKNOWNSee R. Fior et al., «Single - cell functional and chemosensitive profiling of combinatorial colorectal therapy in zebrafish xenografts,» PNAS, doi: 10.1073 / pnas.1618389114, 2017

The ClonTracer library originally came about as Bhang and co-workers were trying to develop a way to quantify the take rate of mouse xenografts after cell implantation.

Most xenograft models for brain tumors using tumor cell lines require at least 10,000 cells.

Finally, I have a category of «other cancers» with exomes published in 2011; these include pancreastic cysts and cell lines, gastric cancer tumors, and prostate cancer samples derived through mouse xenograft models.

Patient - derived xenograft models of non-small cell lung cancer and their potential utility in personalized medicine.

In a xenograft KMT2D - mutated T - lymphoma model, dual treatment with chidamide and decitabine significantly retarded tumor growth and induced cell apoptosis through modulation of the KMT2D / H3K4me axis.

Altogether, these results indicate that the xenograft grew from the patient's Epstein - Barr virus - infected B - lymphoid cells and could be assimilated to posttransplant lymphoproliferative disease.

In a series of lab experiments with cell lines, human xenograft tumors in mice and primary human prostate cancer samples, the researchers demonstrated that miR - 34a inhibits prostate cancer stem cells by suppressing CD44.

They now show that engineering hESC - and iPSC - derived NK cells with a specific HIV CD4ζ chimeric receptor enhances the suppression of HIV replication in vitro and study these cells in a mouse xenograft model [6].

Flavopiridol inhibits several cellular kinases and has demonstrated cytostatic and cytotoxic activity in vitro and in vivo in numerous human tumor cell lines and xenograft models (including human breast, prostate, and lung carcinoma) at clinically achievable concentrations.

It was indeed found DAXX promotes the in vivo tumorigenicity of two human PCa cell lines in a subcutaneous tumor xenograft model via suppression of autophagy [Submitted].

The in vivo anti-tumor activity of dual treatment on T - cell lymphoma was further evaluated in a murine xenograft model in which KMT2D V5486M mutated Jurkat cells subcutaneously injected into nude mice.

PP2A mediates diosmin p53 activation to block HA22T cell proliferation and tumor growth in xenografted nude mice through PI3K - Akt - MDM2 signaling suppression.

We also found that the EphB4 receptor expressed on the surface of breast cancer cells can promote tumor xenograft growth by enhancing blood vessel formation through interactions with its preferred ligand, ephrin - B2, present in tumor endothelial cells.

Specifically, the Ignatius laboratory will study tumorigenic cell populations that self - renew and metastasize using a combination of zebrafish, murine xenograft and human cell culture systems.

Acquired tumor cell resistance to sunitinib causes resistance in a HT - 29 human colon cancer xenograft mouse model without affecting sunitinib biodistribution or the tumor microvasculature

On the basis of studies on the XMRV - producing human prostate cancer cell lines CWR22Rν1 and CWR - R1 and their progenitor tumour xenograft CRW22, they concluded that XMRV infections were caused by contamination during in vivo passaging in nude mice.

The in vivo performance of plasmonic nanobubbles as cell theranostic agents in zebrafish hosting prostate cancer xenografts.

Pearson's R2 correlation was calculated for correlation between xenograft size, HIF - 1α / Glut1, and mammosphere - forming cells (MFC).

To purify tumor cells, PC3 xenograft tumors were aseptically dissected out from animals and minced into ∼ 1 - mm3 pieces in RPMI - 7 % FBS.

On the contrary, recent evidence indicates that XMRV is a contaminant originating from the recombination of two mouse endogenous retroviruses during passaging of a prostate tumor xenograft (CWR22) in mice, generating laboratory - derived cell lines that are XMRV - infected.

We also demonstrate that immunoglobulin - secreting NSCs exhibit preferential tropism to tumor cells in vivo, and can deliver antibodies to human breast cancer xenografts in mice.

In 2011, Paprotka, et al. reported that XMRV likely originated through recombination between 2 endogenous murine retroviruses, PreXMRV - 1 and PreXMRV - 2, during in vivo passaging of the human prostate cancer xenograft CWR - R1, resulting in establishment of the XMRV - infected 22Rv1 cell line [38].

In turn, the LNCaP cells were most likely previously infected by 22Rv1, from which XMRV almost certainly originated through in vivo passaging of the CWR22 xenograft in mice [38].

These platforms include genetically engineered mouse (GEM) models, fallopian tube - derived cell lines, and patient - derived tumor xenografts.

These results clearly indicate that analysis of future xenograft experiments should take careful note of the size of the xenografts as well as how hypoxia influences CSC in the specific cell line used.

Subcutaneous tumor cell implantation and purification of tumor cells from xenograft tumors.

ONC201 combined with bortezomib in a Burkitt's lymphoma xenograft model reduced tumor cell density and improved CHOP induction compared to either agent alone.

Autophagy inhibition synergistically enhances anticancer efficacy of RAMBA, VN / 12 -1 in SKBR - 3 cells, and tumor xenografts.

NSG ™ variants are the most highly immunodeficient mice and the models of choice for cancer xenograft modeling, stem cell...

HCMV and COX - 2 expression correlated in primary tumors, cell lines, and medulloblastoma xenografts.

In his study menopausal mice were injected with xenografted human breast cancer cells.