Sentences with phrase «zebrafish embryos»

Finally, we will test the function of these candidate factors to induce tooth organogenesis in zebrafish embryos.

In a study by Junker et al, the authors studied zebrafish embryos at the 15 somites stage by straightening them, then segmenting them into about 100 slices, each roughly 18 µm thick.

To demonstrate Amrose functionality in an established higher model system, Amrose v0 — v4 variants were cloned into the pCS2 + vector system to synthesize mRNA by in vitro transcription, which was used for injection into one - cell stage zebrafish embryos.

Photostability analysis was performed using three zebrafish embryos each for Amrose v3 and mCherry.

Looking through a microscope, Youngnam Jin — a postdoctoral fellow in Randy Peterson's lab at Massachusetts General Hospital's Cardiovascular Research Center — arranges one - celled zebrafish embryos, each about 10 minutes old, into rows of 20, spinning them with a micro spatula to turn their nuclei toward the ultrathin glass needle of a micro-injection device.

Two stereomicroscopes, an Olympus FVX10, and a Zeiss V12 Discovery are available for transmitted light and epifluorescence imaging of C. elegans, zebrafish embryos and larvae, and other larger species and specimens.

Ulitsky and Shkumatava, who report their findings in this week's issue of the journal Cell, tested the function of two of the 29 lincRNAs by knocking them down in zebrafish embryos.

She saw that cells of older zebrafish embryos didn't glow much when they were exposed to water the December 2014 and June 2015 stormwater, meaning the cells weren't very active.

Intriguingly, abnormal muscle contractions are also observed in muscular dystrophy patients and they were previously reported in zebrafish embryos with a related genetic defect, Panin said.

The topics include finding cancer cells, hand hygiene, zebrafish embryos and DNA code.

In this study, the team delved deep into the nucleus of cells belonging to mouse and zebrafish embryos — two important animal models of embryonic development — in order to determine how the Dll4 gene is turned on.

Like the frog, zebrafish embryos develop externally and can be viewed and manipulated at all stages.

Studies in cultured embryonic stem (ES) cells, and our analysis of zebrafish embryos have revealed that pluripotency is characterized by a unique chromatin signature.

Studies with Zebrafish embryos help scientists understand how whole organisms develop from the single - celled embryo.

To elucidate the roles of eya4 in heart function, we studied zebrafish embryos injected with antisense morpholino oligonucleotides.

Tissue micromanipulation in zebrafish embryos.

At the Bateson Centre at the University of Sheffield, biologists and hospital doctors have teamed up to use zebrafish embryos to learn more about human diseases.

Zebrafish embryos as a model host for the real time analysis of Salmonella typhimurium infections.

Developmental toxicity of CdTe QDs in zebrafish embryos and larvae.

In The Bateson Centre we are identifying genes involved in arteriogenesis using zebrafish embryos.

With the first spontaneous movements taking place as early as 17 hours post-fertilization and swimming behavior occuring after 27 hours, zebrafish embryos prove to be a good model for the study of development of locomotor activity.

In addition to Project BioEYES, the program has expanded to include a new project called Your Watershed, Your Backyard, in which middle - school students grow zebrafish embryos in water samples from their own local watershed to test the effects of pollution.

On display were transparent zebrafish embryos just a few days old.

How zebrafish embryos react to the manipulation of certain compounds (e.g. Ahmad et al., 2012) is measured by the kind of activity they show.

We further validated the pathogenic nature of this mutation by knocking down its expression in zebrafish embryos, which resulted in a developmental defect.

In transient transgenic reporter studies of zebrafish embryos, the +254 kb element holds up: the wild - type allele had enhancer activity in 41/82 embryos (50 %), whereas the mutant allele had enhancer activity in 3/83 embryos (3.6 %).

When the scientists broke up cell huddles in their zebrafish embryos, FGF leaked out.

Microinjection of wild - type human ACVR1 RNA into alk8 — / — zebrafish embryos rescued approximately 80 % of the injected embryos completely or partially (Figure 3, C and F), showing that human ACVR1 can function as a BMP type I receptor in this zebrafish model and substitute for Alk8.

Overexpression of R206H ACVR1 in zebrafish embryos causes strong ventralization by enhancing BMP signaling.

As further evidence of BMP signaling activation, proteins isolated from zebrafish embryos were examined by immunoblotting for phosphorylated Smad1 / 5.

Most notably, the studies are based on transient transfection of wild - type and mutant gene constructs into cultured cells or into zebrafish embryos and are unlikely to reproduce the specific mutant gene dose that is present in heterozygous mutant cells in FOP patients.

Study results published online in the journal Green Chemistry show that the molecules, which are aimed at removing hazardous endocrine disruptors from water sources, aren't endocrine disruptors themselves as they proved to be non-toxic to developing zebrafish embryos.

Using gene therapy to insert the gene, they expressed the synthetic gene in adult mice or in zebrafish embryos, and witnessed remote activation of neurons in the presence of a magnetic field through the altered behavior of the animals.

In the team's studies on zebrafish embryos, the embryos responded to a magnetic field by twirling, which they generally do in response to pressures in the water.

During the course, students collect zebrafish embryos and watch them develop from single cells to swimming larvae complete with beating hearts and distinct pigmentation.

Previous studies have found that mouse and zebrafish embryos do not develop properly in simulated microgravity.

Wherever they injected RNA for the trio into the zebrafish embryos, tails popped up, the team reports in the 24 July issue of Nature.

Surprisingly, the researchers found a similar bimodal 3 - dimensional chromatin architecture in the Hoxd gene region in zebrafish embryos.

But overall, the resulting toxicity of the wastewater due to its silver content was negligible to zebrafish embryos — a model animal used in toxicity studies.

Using gene therapy to insert the gene, they expressed the synthetic gene in adult mice or in zebrafish embryos, and witnessed remote activation of neurons the presence of a magnetic field through the altered behavior of the animals.

In experiments with zebrafish embryos in which the expression of Abcb4 protein was suppressed, Luckenbach and his team found that the embryos were much more sensitive to toxic chemicals and that these substances were enriched to a greater extent in the embryo tissue.

Zebrafish embryos are used for the assessment of chemicals and for investigations of environmental impact, so we hope that in future our study will contribute to an awareness of the need to incorporate Abcb4 transport processes in toxicological testing directives.»

In addition to seeing green lymphatic cells in the zebrafish embryos, the researchers noticed that green cells also covered the surface of the tiny fish's brains.

Zebrafish embryos have transparent brains, which allowed Peri and her team to track the microglia in real time under the microscope.

When the scientists inserted human colorectal cancer cells into zebrafish embryos and allowed them to grow for 4 days, the resulting tumors showed three hallmarks of human solid tumors: rapid cell division, formation of blood vessels to supply nutrients, and the ability to spread to other locations in the body.

The researchers found that maternal gdf3 is required for Ndr1 and Ndr2 to signal at the levels necessary to properly induce the formation of mesoderm and endoderm cells in early zebrafish embryos.

To investigate, Peterson's team exposed zebrafish embryos to thousands of drugs and recorded how each affected their reaction to a flash of light or a slight poke (Nature Chemical Biology, DOI: 10.1038 / nchembio.307).

In the initial stages of the research project, Yaniv's team members Julian Nicenboim and Dr. Guy Malkinson obtained images of developing zebrafish embryos, whose transparent bodies make it possible to document embryonic development in real time over several days.

The design differs from that of a conventional commercial microscope: Keller placed the laser and objective lens at right angles to each other atop a table; then he embedded zebrafish embryos in agarose in a sample tube between the two.

They found that the TAML - treated BPA water did not show estrogen activity or cause abnormalities in yeast and developing zebrafish embryos.