Sentences with phrase «zebrafish genes»

By using multiple RNA guides, researchers are able to «knock out» — that is, effectively delete or turn off — zebrafish genes with close to 90 % efficiency vs. about 5 % efficiency using earlier methods.

Study pursues the genes with which a zebrafish views its world Neuroscientist biologist Herwig Baier, PhD, UCSF assistant professor of physiology and formerly of the Max Planck Institute in Tubingen, is working to identify zebrafish genes that play a role in visual perception.

However, to be able to do this, we need to have as complete as a list of zebrafish genes as possible.

Some mouse and zebrafish genes remain active for up to four days after the animals die, scientists reported in 2017 in Open Biology.

«We studied a zebrafish gene that is analogous to a human gene that causes deafness, and here we show the defect is in the process of mechanotransduction.»

Because of the usefulness of this information, we set ourselves the goals of constructing a zebrafish gene set based on RNA - Seq alone and then identifying the highest quality models.

Not exact matches

For the study, the researchers created a line of zebrafish with defective immune systems by inserting a mutation into a gene required for development of an important component of the immune system.

The centre will be structured around six technical platforms focusing on zebrafish transgenesis and micromanipulation, bioimaging, mouse transgenesis, flow cytometry, stem cell culture, and gene - expression analysis.

The study found that zebrafish that failed to inherit specific genetic instructions from mom developed fatal defects earlier in development, even if the fish could make their own version of the gene.

In the study, Burdine and two graduate students Jose Pelliccia and Granton Jindal used CRISPR / Cas9 gene editing to remove Vg1, known as Gdf3 in zebrafish.

Already, researchers have used CRISPR / Cas9 to edit genes in human cells grown in lab dishes, monkeys (SN: 3/8/14, p. 7), dogs (SN: 11/28/15, p. 16), mice and pigs (SN: 11/14/15, p. 6), yeast, fruit flies, the worm Caenorhabditis elegans, zebrafish, tobacco and rice.

Investigators at Duke University, led by Erica Davis, PhD, a co-senior author of the report, blocked the gene's expression in zebrafish, which resulted in abnormal facial cartilage, smaller eyes and structural abnormalities of neurons involved in the development of reproductive organs.

To gain more insights as to why zebrafish are more successful at regenerating spinal tissue, the researchers analyzed the gene expression of fish spinal tissue following injury, identifying seven genes of interest.

Using a novel combination of technologies, including trio exome sequencing of patient / parental DNA and genetic studies in the tiny larvae of zebrafish, the EuroEPINOMICS RES consortium found that mutations in the gene CHD2 are responsible for a subset of epilepsy patients with symptoms similar to Dravet syndrome — a severe form of childhood epilepsy that is in many patients resistant to currently available anti-epileptic drugs.

Removing a single gene from the brains of mice and zebrafish causes these animals to become more anxious than normal.

«Although zebrafish look quite different from humans, they share an astonishing 70 percent of their genetic material with humans, including genes important for the formation of new heart muscle,» Yin said.

If you actually read the paper that launched a thousand offbeat tidbits, by one Emmanuel Mignot of Stanford University, you discover that those «mutant» zebrafish with the defective genes were engineered to be mutants, in bulk, purely for the purpose of research.

In the case of CHD2, scientists collaborating with the EuroEPINOMICS RES consortium used antisense technology to rapidly generate zebrafish larvae with a partial loss of function of this gene, and were then able to detect epileptic seizures in these animals using electrographic analysis (this method is very similar to electroencephalography, or EEG, which is used to analyze seizures in humans).

The zebrafish larva is an ideal model to study genes involved in epilepsy, and the methods necessary for such studies are now well - established in our laboratory.

Functional analyses indicate that MFSD12 encodes a lysosomal protein that affects melanogenesis in zebrafish and mice, and that mutations in melanocyte - specific regulatory regions near DDB1 / TMEM138 correlate with expression of ultraviolet response genes under selection in Eurasians.

The results from McCammon's initial screen with zebrafish indicate that two genes in the 16p11.2 region could be key for brain development: fam57ba and doc2a.

For the first time, Whitehead Institute scientists have documented a direct link between deletions in two genes — fam57ba and doc2a — in zebrafish and certain brain and body traits, such as seizures, hyperactivity, enlarged head size, and obesity.

Zebrafish also have the PSENEN gene.

Like the human genome, the zebrafish genome has two copies of each gene, and scientists can remove the function of multiple genes to produce phenotypes that are reminiscent of human symptoms.

«In order to be able to research more closely what effect PSENEN has, we, in collaboration with Prof. Odermatt, deactivated the gene in some zebrafish larvae and then compared these with normal larvae under the microscope,» explains Damian Ralser.

Humans and zebrafish share most protein - coding genes, and CTGF is no exception.

When Kaufman, Zon and colleagues looked to see what was different about these early cancer cells, they found that crestin and the other activated genes are the same ones turned on during zebrafish embryonic development — specifically, in the stem cells that give rise to the pigment cells known as melanocytes, within a structure called the neural crest.

Zebrafish can find a way to compensate for a mutated gene, but artificial methods of inactivating the same gene are not so readily overcome, a new study suggests.

When zebrafish with the green fluorescing endothelial gene matured, the researchers observed green FGPs on the surface of the fish's brains — confirming that these cells arose from endothelial tissue.

«In the future, zebrafish will be used to identify new genes and drugs that can influence myelin formation and myelin repair,» says Dr. Lyons.

When the scientists prevented the genes from working in zebrafish, they failed to develop fingerlike projections called fin rays — the fishes» «hands.»

Because these genes have the same function in zebrafish, humans, and other tetrapods, it should help researchers further understand how our ancestors left the water and evolved limbs from fins.

Scientists led by John Kuwada, professor of molecular, cellular and developmental biology at the University of Michigan, and Hiromi Hirata of the National Institute of Genetics in Japan originally identified the gene in mutant zebrafish that exhibited severe muscle weakness.

It is also known that zebrafish and humans have very similar genes, and these similarities extend to more than 80 % of the genes associated with human disease.

To get a better idea of the gene's role in development the researchers blocked the expression of the gene in zebrafish.

This is possible because the zebrafish retina contains cells called Müller glia that harbor a gene that allows them to regenerate.

Using gene therapy to insert the gene, they expressed the synthetic gene in adult mice or in zebrafish embryos, and witnessed remote activation of neurons the presence of a magnetic field through the altered behavior of the animals.

Blood vessels of a zebrafish larva: if the gene EGFL7 is lacking, the blood vessels (stained green) are not formed correctly.

The shared gene allows zebrafish to sense water flow direction, and it also helps cells inside the human ear sense a range of sounds.

McDermott's team discovered hair cells on the zebrafish skin use different mechanotransduction genes — like tmc2b — depending on their orientation.

GENE SCENE Zebrafish experiments that didn't go as planned may help researchers find genes that can fill in for others in a pinch.

Zebrafish share 70 percent of genes with people and also share similar biological pathways that lead to addiction.

In late 2012, the pathologist at Massachusetts General Hospital in Boston assembled the components of the new gene - editing technology and fiddled with the DNA of a zebrafish embryo.

Researchers discovered a gene also found in humans helps zebrafish convert water motion into electrical impulses that are sent to the brain for perception.

«Variable gene expression in zebrafish.»

Using the CAGE (Cap Analysis of Gene Expression) high - throughput method, the scientists determined the starting points of transcription of many thousands of genes in various phases of embryonic development of zebrafish.

For the first time, it is now found by a study that various mechanisms of transcribing DNA into RNA exist during gene expression in the different development phases of zebrafish.

Surprisingly, the researchers found a similar bimodal 3 - dimensional chromatin architecture in the Hoxd gene region in zebrafish embryos.

More than 200 genes in the germ - free zebrafish showed levels of activity different than those in their bacteria - exposed cousins.

«Fish eyes to help understand human inherited blindness: Discovery of a gene in zebrafish that triggers congenital blindness could lead to a suitable cure for similar disease in humans..»