I need help on how to generate a stable neuronal cell line expressing prion protein via pre-transfection restriction enzyme digestion of plasmid DNA using lipid reagent. (blogs.nature.com)
Working with plasmid DNA is much easier and doesn't require the extra precautions needed when dealing with RNA (e.g. face mask, and a dust free and specially cleaned work bench). (blog.addgene.org)
293, 293T, and HepG2 cells were transfected with plasmids of p53 - TA - Luc plus RSV - lacZ, and then incubated with ActD (10 nM) for the indicated time periods, followed by the activity assays of luciferase and β - galactosidase. (oncotarget.com)