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Tubes were spun, the nuclear lysis buffer with proteinase inhibitor was added and sonicated to form shared chromatin of about 200 — 1000 base pairs in length (data not shown). (elifesciences.org)
Make up «direct lysis buffer» by adding 1 mL of Proteinase K (e.g. Sigma P4850) to 100 mL DirectPCR Lysis Reagent (cell)(Viagen Biotech, Cat # 302 - C). (blog.horizondiscovery.com)
Related sites MRC Protein Group, University of Alberta Aspartic proteinase advanced tutorial (sciencemag.org)