Treatment with ActD (10 nM) distinctly induced the expression and phosphorylation of p53 at 18 h, reaching a maximal response at 24 h, and maintaining a high level of p53 for up to 30 h in the 293 and 293T cells (Fig. 1A). (oncotarget.com)
In contrast, treatment with ActD (10 nM) distinctly induced the expression and phosphorylation of p53 at 3 h, reaching a maximal response at 6 h, and maintaining a high level of p53 for up to 12 h in the HepG2 cells. (oncotarget.com)
However, at the time of the maximal response, less than 125 ml water remain in the stomach (19). (academic.oup.com)