Here, we show that a non-adherent, stem - like, and metastatic CSC - enriched subpopulation could be isolated by exposing human metastatic breast cancer cell lines to cycles of chronic hypoxia followed by reoxygenation. (breast-cancer-research.biomedcentral.com)
Mammosphere culture of metastatic breast cancer cells enriches for tumorigenic breast cancer cells (cancerres.aacrjournals.org)
Conditions for hypoxia / reoxygenation cycles were optimized using two human metastatic breast cancer cell lines (MDA - MB 231 and BCM2). (breast-cancer-research.biomedcentral.com)