Definition of «immunoprecipitation»

Immunoprecipitation is a laboratory technique used to isolate specific proteins from a complex mixture, such as cell lysates or tissue extracts. This method involves the use of antibodies that are specifically bound to their target protein (the one you want to study). The antigen-antibody complex is then precipitated out of solution using an agent like salt or acetone, which causes the proteins to aggregate and can be easily separated from the rest of the mixture. This technique allows researchers to selectively isolate specific proteins for further analysis such as identification, quantification, or functional studies.

Phrases with «immunoprecipitation»

Sentences with «immunoprecipitation»

  • Normal rabbit IgG (Millipore) was used to control the nonspecific immunoprecipitation of chromatin by immunoglobulins. (cancerres.aacrjournals.org)
  • To evaluate histone modification (another epigenetic event), the authors employed chromatin immunoprecipitation with high - throughput sequencing (CHiP - Seq) to capture DNA sequences bound by H3 histones marking active (K4me1, K4me3, K27ac, K36me3) or repressed (K27me3, K9me3) chromatin. (massgenomics.org)
  • In the study, the team performed a genome - wide chromatin immunoprecipitation analysis for genes that were the target of BMAL1, a core clock component that binds to many other clock genes, regulating their transcription. (sciencedaily.com)
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