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To detect the expression of p53, the HepG2 cells were seeded at 1 × 106 cells / well on microscope cover glasses in 6 - well plates overnight before being treated with the drugs for the appropriate time periods, followed by washing with phosphate - buffered saline and fixing with ethanol. (oncotarget.com)
The cells were then cultured with the test compounds for the appropriate time periods. (oncotarget.com)