High - efficiency counterselection recombineering for site - directed mutagenesis in bacterial artificial chromosomes. (dresden-ipp.de)
These improvements push the boundaries of nucleic - acid detection and fragment sizing, affecting applications such as single - cell nucleic acid analysis, PCR - free preparation of NGS library preparation, bacterial artificial chromosome clone sizing, and exosome analysis. (sciencemag.org)
Overlapping «cassettes» of 5 to 7 kilobases (kb), assembled from chemically synthesized oligonucleotides, were joined by in vitro recombination to produce intermediate assemblies of approximately 24 kb, 72 kb («1/8 genome»), and 144 kb («1/4 genome»), which were all cloned as bacterial artificial chromosomes in Escherichia coli. (science.sciencemag.org)