Successive high - speed AFM images of bacteriorhodopsin proteins adsorbed onto a mica surface. (kavliprize.org)
They validated the result by x-ray analysis of embedded bacteriorhodopsin protein at 4 °C. (science.sciencemag.org)
Then in 1990, after 15 years» work refining sample preparation and electron detection, Henderson succeeded in using an electron microscope to create an image of a large bacterial cell membrane protein called bacteriorhodopsin, and do it at atomic resolution. (scientificamerican.com)