Bisulfite sequencing is a technique used in genetics and molecular biology to study DNA and how it is modified. It helps to identify and understand chemical changes (methylation) in DNA that can affect gene activity and function. Full definition
ENCODE used reduced representation bisulfite sequencing (RRBS) to profile DNA methylation at 1.2 million CpGs in each of 82 cell lines, finding that: (massgenomics.org)
(B) Bisulfite sequence analysis of the human OCT3 and NANOG promoters in hESCs and multiple iPS clones. (journals.plos.org)
Recovered DNA is suitable for numerous downstream applications, including bisulfite sequencing, NGS, and quantitative PCR. (sciencemag.org)