Compounds were kept in DMSO stock and diluted to a final concentration of 1 % DMSO in complete medium for treatment. (journals.plos.org)
A single - cell suspension (50 μl) containing 1500 cells was mixed with Matrigel (1:1) and plated on top of the Matrigel base onto wells of a 96 - well plate; 50 μl of complete medium was added, and the cells were cultured for 14 days. (stke.sciencemag.org)
Cultures were maintained in complete medium consisting of Neurobasal medium (Invitrogen, Carlsbad, CA) supplemented with B - 27 (Invitrogen), 1 % FBS (Hyclone, Logan, UT), 0.4 mm l - glutamine (Invitrogen), 2.5 gm / l glucose, and 10 ng / ml 2.5 S nerve growth factor (Becton Dickinson, Bedford, MA). (jneurosci.org)