Main Outcomes and Measures Salivary cortisol samples collected at waking and bedtime for children on 3 separate days. (jamanetwork.com)
Second, the 2 cortisol samples collected per day did not allow us to examine the cortisol awakening response (the difference in cortisol levels from waking to 30 minutes later) or to obtain a sensitive measure of total area under the curve across the day, both of which add unique information about the functioning of the HPA axis. (jamanetwork.com)
Finally, all participants returned to the lab five days later, to repeat the questionnaires, report their social movements during the intervening Sunday, and provide one further saliva cortisol sample. (businessinsider.com)